Research progress of single cell transcriptomics in ocular fundus diseases_Chinese Journal of Ocular Fundus Diseases

Authors：

Mao Xiying , Yin Wenjie , Xu Miao , Yuan Songtao ,  Liu Qinghuai

Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

Corresponding author：

Liu Qinghuai, Email: liuqh@njmu.edu.cn

Keywords：

Single-cell RNA sequencing; Ocular fundus diseases; Shared database; Review

DOI：

10.3760/cma.j.cn511434-20230626-00283

Video：

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Abstract Full text Figures/Tables Video References Cited by

Single cell RNA sequencing technique provides a strong technical support for the analysis of cell heterogeneity in biological tissues, and has been widely used in biomedical research. In recent years, considerable scRNA-seq data have been accumulated in the research of ocular fundus diseases. The ocular fundus is abundant for the network of vessel and neuron, which leads to the complicated pathogenesis of fundus diseases. Through single cell RNA sequencing technique, the expression of thousands of genes of certain cell types or even subtypes can be obtained in the disease environment. Single cell RNA sequencing technique accurately reveals the pathogenic cell types and pathogenic mechanisms of ocular fundus diseases such as neovascular retinopathy, which provides a theoretical basis for the birth of new diagnosis and treatment targets. The construction of multi-omics single-cell database of ocular fundus diseases will enable high-quality data to be further explored and provide an analysis platform for ophthalmic researchers.

Citation： Mao Xiying, Yin Wenjie, Xu Miao, Yuan Songtao, Liu Qinghuai. Research progress of single cell transcriptomics in ocular fundus diseases. Chinese Journal of Ocular Fundus Diseases, 2024, 40(3): 243-246. doi: 10.3760/cma.j.cn511434-20230626-00283 Copy

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2. Metzker ML. Sequencing technologies-the next generation[J]. Nat Rev Genet, 2010, 11(1): 31-46. DOI: 10.1038/nrg2626.
3. Stapley J, Reger J, Feulner PG, et al. Adaptation genomics: the next generation[J]. Trends Ecol Evol, 2010, 25(12): 705-712. DOI: 10.1016/j.tree.2010.09.002.
4. Papalexi E, Satija R. Single-cell RNA sequencing to explore immune cell heterogeneity[J]. Nat Rev Immunol, 2018, 18(1): 35-45. DOI: 10.1038/nri.2017.76.
5. Haque A, Engel J, Teichmann SA, et al. A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications[J]. Genome Med, 2017, 9(1): 75. DOI: 10.1186/s13073-017-0467-4.
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7. Voigt AP, Mullin NK, Stone EM, et al. Single-cell RNA sequencing in vision research: insights into human retinal health and disease[J/OL]. Prog Retin Eye Res, 2021, 83: 100934[2020-12-28]. https://pubmed.ncbi.nlm.nih.gov/33383180/. DOI: 10.1016/j.preteyeres.2020.100934.
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Chinese Journal of Ocular Fundus Diseases

Research progress of single cell transcriptomics in ocular fundus diseases

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