Expression of interleukin-17, interleukin-4 and interferon γ in experimental autoimmune uveoretinitis_Chinese Journal of Ocular Fundus Diseases

Authors：

HouBin , ShenLin ,  WangHong , 陈海婷 , 赵萌

Beijing Tongren Eye Center, Beijing Tongren Hospital, [JP2]Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing 100730, China;

Corresponding author：

WangHong, Email: wanghongyk@sina.com

Keywords：

Uveitis/physiopathology; Th17 cells; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2014.04.013

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Objective To observe the expression of interleukin (IL)-17, IL-4 and interferon γ (IFN-γ) in experimental autoimmune uveoretinitis (EAU). Methods C57BL/6 mice were immunized with interphotoreceptor retinoid-binding protein 1-20 to induce EAU. The inflammatory reaction before and on 7, 14, 21, 28 days after immunization were observed. The level of IL-17, IL-4 and IFN-γ in the serum were measured by enzyme-linked immune sorbent assay (ELISA). mRNA and protein expression of spleen and retina were analysed using quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot at the same time, respectively. Results The most serious inflammatory reaction occurred at the 14th day after immunization. The highest level of IFN-γ in serum, highest mRNA and protein expression of IFN-γ in spleen and retina of mice occurred at day 7 after being immunized. The highest level of IL-17, IL-4 in serum, highest mRNA and protein expression of IL-17, IL-4 in spleen and retina of mice occurred at day 14 after being immunized. The increase degree of IL-17 was more than IFN-γ and IL-4. At 7, 14 and 21 days after immunization, compared with the pre-immunization, the level of IL-17, IL-4, IFN-γ in serum of mice were significantly increased (F=1 817.346, 268.600, 164.621; P < 0.05). There was no difference in the levels of IL-17, IL-4, IFN-γin serum of mice between pre-and 28 days after immunization (P > 0.05). At 7, 14 and 21 days after immunization, compared with the pre-immunization, the protein expression of IL-17, IL-4, IFN-γ in spleen (F=312.67, 114.250, 216.220) and retina (F=271.504, 85.370, 80.722) of mice were significantly increased (P < 0.05). There was no difference in protein expression of IL-17, IL-4, IFN-γ in spleen and retina of mice between pre-and 28 days after immunization (P > 0.05). Conclusions There were IL-17, IL-4 and IFN-γ expression in EAU. IL-17, IL-4 and IFN-γ play a key role in the occurrence and development of the EAU.

Citation： HouBin, ShenLin, WangHong. Expression of interleukin-17, interleukin-4 and interferon γ in experimental autoimmune uveoretinitis. Chinese Journal of Ocular Fundus Diseases, 2014, 30(4): 386-389. doi: 10.3760/cma.j.issn.1005-1015.2014.04.013 Copy

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1. Park H, Li Z, Yang XO, et al. A distinct lineage of CD4⁺T cells regulates tissue inflammation by producing interleukin 17[J]. Nat Immunol, 2005, 6:1133-1141.
2. Caspi RR. Th1 and Th2 responses in pathogenesis and regulation of experimental autoimmune uveoretinitis[J]. Int Rev Immunol, 2002, 21: 197-208.
3. Peng Y, Han G, Shao H, et al. Characterization of IL-17⁺ interphotoreceptor retinoid-binding protein-specific T cells in experimental autoimmune uveitis[J]. Invest Ophthalmol Vis Sci, 2007, 48:4153-4161.
4. Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acidsequences[J]. Proc Natl Acad Sci USA, 1981, 78:3824-3828.
5. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 Method[J]. Methods, 2001, 25:402-408.
6. Langrish CL, Chen Y, Blumenschein WM, et al. IL-23 drives a pathogenic T cell population that induces autommune inflammation[J]. Exp Med, 2005, 201:233-240.
7. Lohr J, Knoechel B, Caretto D, et al. Balance of Th1 and Th17 effector and peripheral regulatory T cells[J]. Microbes Infect, 2009, 11:589-593.
8. Komiyama Y, Nakae S, Matsuki T, et al. IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis[J]. J Immunol, 2006, 177: 566-573.

Chinese Journal of Ocular Fundus Diseases

Expression of interleukin-17, interleukin-4 and interferon γ in experimental autoimmune uveoretinitis

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