Objective To evaluate the effect of form deprivation myopia on optic nerve head and retinal morphology in guinea pigs using optical coherence tomography (OCT).
Methods Twenty guinea pigs aged from 4 to 5 weeks were chosen and randomly divided into the experimental group and control group, with 10 guinea pigs in each group. Form deprivation myopia was established for the right eyes of guinea pigs in experimental group for 4 weeks. The guinea pigs of control group were not intervened. Before and 4 weeks after form deprivation, refraction was measured by retinoscopy after cycloplegia; the axial length was measured by A-scan ultrasound; retinal nerve fiber layer (RNFL) thickness, optic nerve head and retinal morphology of guinea pigs were analyzed using OCT.
Results Before form deprivation, there were no statistically significant differences in spherical equivalent, axial length, RNFL thickness, disc edge area, optic disc area, average cup disc ratio, vertical cup disc ratio, cup volume, retinal thickness, or retinal volume between the experimental group and control group of guinea pig (P > 0.05). After 4 weeks of form deprivation, RNFL thickness of (64.9±17.7) μm in guinea pigs in experimental group was thinner compared to (97.9±25.1) μm in control group (t=-2.845, P=0.015). Retinal thickness of (142.7±3.4) μm in guinea pigs in experimental group was thicker compared to (138.4±3.5) μm in control group (t=2.338, P=0.038). There were no significant differences in disc edge area, optic disc area, average cup disc ratio, vertical cup disc ratio, cup volume or retinal volume between groups (P > 0.05). There were statistically significant differences in spherical equivalent, axial length, RNFL thickness, vertical cup to disc ratio cup volume, and retinal thickness between after and before form deprivation in the right eye of guinea pigs in the experimental groups (t=46.001, -50.119, 5.385, 3.447, -2.814, -8.911; P < 0.05), while there were no statistically significant differences in disc edge area, optic disc area, average cup disc ratio, or retinal volume (P > 0.05).
Conclusion Form deprivation myopia has an effect on RNFL and retinal thickness.
Citation：
LiTao, ZhouXiaodong, ZhuJie. Effect of form-deprivation myopia on optic nerve head and retinal morphology in guinea pigs. Chinese Journal of Ocular Fundus Diseases, 2015, 31(5): 477-480. doi: 10.3760/cma.j.issn.1005-1015.2015.05.016
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Copyright © the editorial department of Chinese Journal of Ocular Fundus Diseases of West China Medical Publisher. All rights reserved
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- 1. Li T, Zhou XD, Chen Z, et al. Effects of cyclopentolate on form deprivation myopia in guinea pigs[J]. Open J Ophthalmol, 2015, 5:10-18.
- 2. 欧波.高眼压豚鼠视神经损害的超微结构研究[J].中国热带医学, 2003, 3(3):352-353.
- 3. 李涛, 周晓东, 江瀚然, 等.屈光度和眼轴长度对豚鼠视网膜神经纤维层厚度和视乳头形态的影响[J].中国实验动物学报, 2015(3):285-290.
- 4. 李涛, 周晓东, 崔心瀚, 等.豚鼠眼形觉剥夺后恢复期的生物学参数变化规律探讨[J].中国实验动物学报, 2012, 20(6):51-56.
- 5. 游启生.近视是青光眼发病的危险因素[J].中华眼科杂志, 2014(5):329-330.
- 6. 李翊.应用光学相干断层扫描检测豚鼠眼底结构形态[J].国际眼科杂志, 2011, 11(10):1722-1723.
- 7. Othman SF, Sharanjeet-Kaur, Manan FA, et al. Macular thickness as determined by optical coherence tomography in relation to degree of myopia, axial length and vitreous chamber depth in Malay subjects[J]. Clin Exp Optom, 2012, 95(5):484-491.
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