Study on the Differentiation of Human Mesenchymal Stem Cells into Vascular Endothelial-like Cells_Journal of Biomedical Engineering

Authors：

 XURong ¹ , XUJinyong ² , LIUWei ¹

1. Department of Pharmacy, Anhui Medical College, Hefei 230601, China;
2. Department of Anatomy, Anhui Medical University, Hefei 230032, China;

Corresponding author：

XURong, Email: xurong196810@163.com

Keywords：

human mesenchymal stem cell; vascular endothelial-like cell; culture; differentiation

DOI：

10.7507/1001-5515.20140073

Video：

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Abstract Full text Figures/Tables Video References Cited by

To explore the feasibility of mesenchymal stem cells (MSCs) acting as seed cells in tissue engineering, we isolated human bone marrow MSCs and differentiated them into vascular endothelial-like cells (ELCs) in vitro. Bone marrow mononuclear cells (BMSCs) were isolated by the method of percoll density centrifugation, and seeded in Dulbecco Modified Eagle Medium supplemented with 10% fetal bovine serum. MSCs were purified through multiple adherent cultures, and differentiated into ELCs induced by endothelial cell growth medium-2 (EBM-2) medium containing vascular endothelial growth factor (VEGF), human fibroblast growth factor (hFGF), insulin like growth factors 1 (IGF-1), and human epidermal growth factor (hEGF). The relative biologic characteristics of ELCs including cell morphology and phenotype were studied by inverted microscope and flow cytometry. The induced cells were identified by immunofluorescence with CD31 and Von Willebrand factor (vWF). The results showed that the morphology of MSCs was long-spindle and vortex-like growth. After induction of differentiation, the cells were round, and similar to vascular endothelial cells (ECs). Flow cytometric analysis revealed that ELCs expressed ECs specific surface markers of CD31 and vascular endothelial cadherin (VE-cadherin), but not CD133. Immunofluorescence results also confirmed that ELCs expressed CD31 and vWF. The results suggested that ELCs possed similar cell biological characteristics with ECs. In one word, human MSCs derived from bone marrow have the potential to differentiate into ECs in vitro,and show clinical feasibility acting as ideal donor cells of vascular tissue engineering.

Citation： XURong, XUJinyong, LIUWei. Study on the Differentiation of Human Mesenchymal Stem Cells into Vascular Endothelial-like Cells. Journal of Biomedical Engineering, 2014, 31(2): 389-393. doi: 10.7507/1001-5515.20140073 Copy

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1. PROULX M K,CAREY S P,DITROIA L M,et al.Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential[J].J Biomed Mater Res A,2011,96(2):301-312.
2. KARABEKIAN Z,POSNACK N G,SARVAZYAN N.Immunological barriers to stem-cell based cardiac repair[J].Stem Cell Rev,2011,7(2):315-325.
3. SALES K M,SALACINSKI H J,ALOBAID N,et al.Advancing vascular tissue engineering:the role of stem cell technology[J].Trends Biotechnol,2005,23(9):461-467.
4. WU K,LIU Y L,CUI B,et al.Application of stem cells for cardiovascular grafts tissue engineering[J].Transpl Immunol,2006,16(1):1-7.
5. LI C M,WANG Z G,GU Y Q,et al.Preliminary investigation of seeding mesenchymal stem cells on biodegradable scaffolds for vascular tissue engineering in vitro[J].ASAIO J,2009,55(6):614-619.
6. O'CEARBHAILL E D,MURPHY M,BARRY F,et al.Behavior of human mesenchymal stem cells in fibrin-based vascular tissue engineering constructs[J].Ann Biomed Eng,2010,38(3):649-657.
7. TATEISHI-YUYAMA E,MATSUBARA H,MUROHARA T,et al.Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells:a pilot study and a randomised controlled trial[J].Lancet,2002,360(9331):427-435.
8. FERRO F,SPELAT R,FALINI G,et al.Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure[J].Am J Pathol,2011,178(5):2299-2310.
9. HAN J,KOH Y J,MOON H R,et al.Adipose tissue is an extramedullary reservoir for functional hematopoietic stem and progenitor cells[J].Blood,2010,115(5):957-964.
10. WANG Q R,WANG B H,HUANG Y H,et al.Purification and growth of endothelial progenitor cells from murine bone marrow mononuclear cells[J].J Cell Biochem,2008,103(1):21-29.
11. KUWANA M,OKAZAKI Y,KODAMA H,et al.Endothelial differentiation potential of human monocyte-derived multipotential cells[J].Stem Cells,2006,24(12):2733-2743.
12. KANNO S,ODA N,ABE M,et al.Roles of two VEGF receptors,Flt-1 and KDR,in the signal transduction of VEGF effects in human vascular endothelial cells[J].Oncogene,2000,19(17):2138-2146.

Journal of Biomedical Engineering

Study on the Differentiation of Human Mesenchymal Stem Cells into Vascular Endothelial-like Cells

Abstract Full text Figures/Tables Video References Cited by

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