Hippocampal Neuron Damage and Cognitive Dysfunction of Diabetic Wistar Rats_Journal of Biomedical Engineering

Authors：

 XUEHongyu ^1,2 , WANGJunbo ¹ , ZHUANGYuxia ³ , GAOGuizhen ²

1. School of Life Science and Medicine, Dalian University of Technology, Panjin 124221, China;
2. School of Biological and Chemical Engineering, Suzhou University, Suzhou 234000, China;
3. General Hospital of China Coal Construction, Suzhou 234000, China;

Corresponding author：

XUEHongyu, Email: hongyu_xue@163.com

Keywords：

streptozotocin; diabetic encephalopathy; apoptosis

DOI：

10.7507/1001-5515.20140247

Video：

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This study aimed to explore the cognitive dysfunction of and hippocampal neuron damage to Wistar rats with STZ-induced diabetes at different morbidity time. All Wistar rats in the tests received intraperitoneal injections of streptozotocin (STZ; 60 mg/kg) to induce type 1 diabetes. The concentration of blood glucose and the body weight were investigated, the cognitive ability of rats was assessed using a standardized Y-maze, and the apoptotic neurons in the CA1 of the hippocampus were also examined by using the HE staining. While the sickening time was prolonged, the blood glucose concentration of the experimental rats increased continuously and the body weight decreased. On the 70th day after STZ administration, the neuronal loss in the hippocampal CA1 region increased and the working errors increased in rats with the diabetes. The results showed that Wistar rats could complicate with diabetic encephalopathy in 70 days after injection of STZ for inducing the diabetes.

Citation： XUEHongyu, WANGJunbo, ZHUANGYuxia, GAOGuizhen. Hippocampal Neuron Damage and Cognitive Dysfunction of Diabetic Wistar Rats. Journal of Biomedical Engineering, 2014, 31(6): 1305-1309. doi: 10.7507/1001-5515.20140247 Copy

1.	KNOPMAN D, BOLAND L L, MOSLEY T, et al. Cardiovascular risk factors and cognitive decline in middle-aged adults[J]. Neurology, 2001, 56(1):42-48.
2.	LI Z G, ZHANG W X, SIMA A A F. The role of impaired insulin/IGF action in primary diabetic encephalopathy[J]. Brain Res, 2005, 1037(1-2):12-24.
3.	RYAN C M. Neurobehavioral complications of type Ⅰ diabetes. Examination of possible risk factors[J]. Diabetes Care, 1988, 11(1):86-93.
4.	SCHOENLE E J, SCHOENLE D, MOLINARI L, et al. Impaired intellectual development in children with Type Ⅰ diabetes:association with HbA(1c), age at diagnosis and sex[J]. Diabetologia, 2002, 45(1):108-114.
5.	LI Z G, SIMA A A F. C-peptide and central nervous system complications in diabetes[J]. Exp Diabesity Res, 2004, 5(1):79-90.
6.	PARADIES G, PETROSILLO G, PARADIES V, et al. Mitochondrial dysfunction in brain aging:role of oxidative stress and cardiolipin[J]. Neurochem Int, 2011, 58(4):447-457.
7.	RAO Y, XIAO P, XU S T. Effects of intrahippocampal aniracetam treatment on Y-maze avoidance learning performance and behavioral long-term potentiation in dentate gyrus in rat[J]. Neurosci Lett, 2001, 298(3):183-186.
8.	SRINIVASAN S, STEVENS M, WILEY J W. Diabetic peripheral neuropathy:evidence for apoptosis and associated mitochondrial dysfunction[J]. Diabetes, 2000, 49(11):1932-1938.
9.	BIESSELS G J, KAMAL A, URBAN I J, et al. Water maze learning and hippocampal synaptic plasticity in streptozotocin-diabetic rats:effects of insulin treatment[J]. Brain Res, 1998, 800(1):125-135.
10.	SIMA A A, GARCIA-SALINAS R, BASU P K. The BB wistar rat:an experimental model for the study of diabetic retinopathy[J]. Metabolism, 1983, 32(7 Suppl 1):136-140.
11.	AGRAWAL S S, NAQVI S, GUPTA S K, et al. Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms[J]. Food Chem Toxicol, 2012, 50(9):3126-3132.
12.	ASNAGHI V, GERHARDINGER C, HOEHN T, et al. A role for the polyol pathway in the early neuroretinal apoptosis and glial changes induced by diabetes in the rat[J]. Diabetes, 2003, 52(2):506-511.
13.	ANDERSEN M B, SAMS-DODD F. Transient cerebral ischemia inhibits juvenile recognition in the Mongolian gerbil[J]. Pharmacol Biochem Behav, 1997, 56(4):719-725.
14.	SARAVIA F E, BEAUQUIS J, REVSIN Y, et al. Hippocampal neuropathology of diabetes mellitus is relieved by estrogen treatment[J]. Cell Mol Neurobiol, 2006, 26(4-6):943-957.

1. KNOPMAN D, BOLAND L L, MOSLEY T, et al. Cardiovascular risk factors and cognitive decline in middle-aged adults[J]. Neurology, 2001, 56(1):42-48.
2. LI Z G, ZHANG W X, SIMA A A F. The role of impaired insulin/IGF action in primary diabetic encephalopathy[J]. Brain Res, 2005, 1037(1-2):12-24.
3. RYAN C M. Neurobehavioral complications of type Ⅰ diabetes. Examination of possible risk factors[J]. Diabetes Care, 1988, 11(1):86-93.
4. SCHOENLE E J, SCHOENLE D, MOLINARI L, et al. Impaired intellectual development in children with Type Ⅰ diabetes:association with HbA(1c), age at diagnosis and sex[J]. Diabetologia, 2002, 45(1):108-114.
5. LI Z G, SIMA A A F. C-peptide and central nervous system complications in diabetes[J]. Exp Diabesity Res, 2004, 5(1):79-90.
6. PARADIES G, PETROSILLO G, PARADIES V, et al. Mitochondrial dysfunction in brain aging:role of oxidative stress and cardiolipin[J]. Neurochem Int, 2011, 58(4):447-457.
7. RAO Y, XIAO P, XU S T. Effects of intrahippocampal aniracetam treatment on Y-maze avoidance learning performance and behavioral long-term potentiation in dentate gyrus in rat[J]. Neurosci Lett, 2001, 298(3):183-186.
8. SRINIVASAN S, STEVENS M, WILEY J W. Diabetic peripheral neuropathy:evidence for apoptosis and associated mitochondrial dysfunction[J]. Diabetes, 2000, 49(11):1932-1938.
9. BIESSELS G J, KAMAL A, URBAN I J, et al. Water maze learning and hippocampal synaptic plasticity in streptozotocin-diabetic rats:effects of insulin treatment[J]. Brain Res, 1998, 800(1):125-135.
10. SIMA A A, GARCIA-SALINAS R, BASU P K. The BB wistar rat:an experimental model for the study of diabetic retinopathy[J]. Metabolism, 1983, 32(7 Suppl 1):136-140.
11. AGRAWAL S S, NAQVI S, GUPTA S K, et al. Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms[J]. Food Chem Toxicol, 2012, 50(9):3126-3132.
12. ASNAGHI V, GERHARDINGER C, HOEHN T, et al. A role for the polyol pathway in the early neuroretinal apoptosis and glial changes induced by diabetes in the rat[J]. Diabetes, 2003, 52(2):506-511.
13. ANDERSEN M B, SAMS-DODD F. Transient cerebral ischemia inhibits juvenile recognition in the Mongolian gerbil[J]. Pharmacol Biochem Behav, 1997, 56(4):719-725.
14. SARAVIA F E, BEAUQUIS J, REVSIN Y, et al. Hippocampal neuropathology of diabetes mellitus is relieved by estrogen treatment[J]. Cell Mol Neurobiol, 2006, 26(4-6):943-957.

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