A study on the regulation of motor behavior in mouse based on temporal interference_Journal of Biomedical Engineering

Authors：

ZHU Haoran ¹ , HUAI Ruituo ¹ , ZHANG Pingqiu ¹ , WANG Hui ¹ , YANG Junqing ¹ , YIN Tao ¹ ,  YU Zhihao ² , SHAO Feng ³

1. College of Electrical Engineering and Automation, Shandong University of Science and Technology, Qingdao, Shangdong 266510, P. R. China;
2. College of Energy Storage Technology, Shandong University of Science and Technology, Qingdao, Shangdong 266510, P. R. China;
3. Qingdao Jinmotang Biotechnology Co., Ltd, Qingdao, Shangdong 266510, P. R. China;

Corresponding author：

YU Zhihao, Email: zhihaoa@163.com

Keywords：

Temporal interference; Behavioral regulation; Ventral posterior lateral nucleus of the thalamus; Stimulation current

DOI：

10.7507/1001-5515.202305032

Video：

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Temporal interference (TI) as a new neuromodulation technique can be applied to non-invasive deep brain stimulation. In order to verify its effectiveness in the regulation of motor behavior in animals, this paper uses the TI method to focus the envelope electric field to the ventral posterior lateral nucleus (VPL) of the thalamus in the deep brain of mouse to regulate left- and right-turning motor behavior. The focusability of TI in the mouse VPL was analyzed by finite element method, and the focus area and volume were obtained by numerical calculation. A stimulator was used to generate TI current to stimulate the mouse VPL to verify the effectiveness of the TI stimulation method, and the accuracy of the focus location was further determined by c-Fos immunofluorescence experiments. The results showed that the electric field generated by TI stimulation was able to focus on the VPL nuclei when the stimulation current reached 800 μA; the mouse were able to make corresponding left and right turns according to the stimulation position; and the c-Fos positive cell markers in the VPL nuclei increased significantly after stimulation. This study confirms the feasibility of TI in regulating animal motor behavior and provides a non-invasive stimulation method for brain tissue for animal robots.

Citation： ZHU Haoran, HUAI Ruituo, ZHANG Pingqiu, WANG Hui, YANG Junqing, YIN Tao, YU Zhihao, SHAO Feng. A study on the regulation of motor behavior in mouse based on temporal interference. Journal of Biomedical Engineering, 2024, 41(2): 342-350. doi: 10.7507/1001-5515.202305032 Copy

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24.	王浩, 王绍康, 邱兆成, 等. 户外鸽子生物机器人的设计及初步应用. 生物医学工程学杂志, 2022, 39(6): 1209-1217.
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26.	Guo W, He Y, Zhang W, et al. A novel non-invasive brain stimulation technique: “Temporally interfering electrical stimulation”. Front Neurosci, 2023, 17: 1092539.
27.	Zhang Z, Lin B S, Wu C W G, et al. Designing and pilot testing a novel transcranial temporal interference stimulation device for neuromodulation. IEEE T Neur Sys Reh, 2022, 30: 1483-1493.

1. 苏学成, 槐瑞托, 杨俊卿, 等. 控制动物机器人运动行为的脑机制和控制方法. 中国科学: 信息科学, 2012, 42(9): 1130-1146.
2. 郭策, 戴振东, 孙久荣. 生物机器人的研究现状及其未来发展. 机器人, 2005(2): 187-192.
3. Talwar S K, Xu S, Hawley E S, et al. Rat navigation guided by remote control. Nature, 2002, 417(6884): 37-38.
4. Yang J, Huai R, Wang H, et al. A robo-pigeon based on an innovative multi-mode telestimulation system. Biomed Mater Eng, 2015, 26(s1): S357-S363.
5. 彭勇, 王婷婷, 闫艳红, 等. 鲤鱼机器人无线遥控系统设计与应用. 中国生物医学工程学报, 2019, 38(4): 431-437.
6. Feng Z, Chen W, Ye X, et al. A remote control training system for rat navigation in complicated environment. J Zhejiang Uni Sci A, 2007, 8: 323-330.
7. 伊国胜, 焦立峰, 王江, 等. 深部脑刺激的逆向激活效应及其研究进展. 中国生物医学工程学报, 2021, 40(3): 364-374.
8. Vissani M, Isaias I U, Mazzoni A. Deep brain stimulation: a review of the open neural engineering challenges. J Neural Eng, 2020, 17(5): 051002.
9. Pi X, Li S, Xu L, et al. A preliminary study of the noninvasive remote control system for rat bio-robot. J. Bionic Eng, 2010, 7(4): 375-381.
10. 彭勇, 韩晓晓, 王婷婷, 等. 一种用于鲤鱼机器人的光刺激装置及光控实验方法. 生物医学工程学杂志, 2018, 35(5): 720-726.
11. 彭勇, 赵洋, 张乾, 等. 不同波长的光对鲤鱼机器人运动行为影响的研究. 生物医学工程学杂志, 2021, 38(4): 647-654.
12. Kim D G, Lee S, Kim C H, et al. Parasitic robot system for waypoint navigation of turtle. J Bionic Eng, 2017, 14(2): 327-335.
13. Grossman N, Bono D, Dedic N, et al. Noninvasive deep brain stimulation via temporally interfering electric fields. Cell, 2017, 169(6): 1029-1041.
14. Rampersad S, Roig-Solvas B, Yarossi M, et al. Prospects for transcranial temporal interference stimulation in humans: a computational study. NeuroImage, 2019, 202: 116124.
15. Gomez-Tames J, Asai A, Hirata A. Multiscale computational model reveals nerve response in a mouse model for temporal interference brain stimulation. Front Neurosci, 2021, 15: 684465.
16. Song X, Zhao X, Li X, et al. Multi-channel transcranial temporally interfering stimulation (tTIS): application to living mice brain. J Neur Eng, 2021, 18(3): 036003.
17. Missey F, Rusina E, Acerbo E, et al. Orientation of temporal interference for non-invasive deep brain stimulation in epilepsy. Front Neurosci, 2021, 15: 656.
18. Missey F, Donahue M J, Weber P, et al. Laser‐driven wireless deep brain stimulation using temporal interference and organic electrolytic photocapacitors. Adv Funct, 2022, 32(33): 2200691.
19. Wang Q, Ding S L, Li Y, et al. The Allen mouse brain common coordinate framework: a 3D reference atlas. Cell, 2020, 181(4): 936-953. e20.
20. 熊慧, 景昭, 刘近贞. 新型经颅磁刺激三层-8字形线圈的结构设计. 浙江大学学报(工学版), 2021, 55(4): 793-800.
21. Koponen L M, Nieminen J O, Ilmoniemi R J. Minimum-energy coils for transcranial magnetic stimulation: application to focal stimulation. Brain Stimul, 2015, 8(1): 124-134.
22. Huai R, Yang J, Wang H, et al. A new robo-animals navigation method guided by the remote control// 2009 2nd International Conference on Biomedical Engineering and Informatics. Tianjin: IEEE, 2009: 1-4.
23. 王勇, 槐瑞托, 王敏, 等. 基于脑微刺激的智能动物的研究. 中国生物医学工程学报, 2006(4): 497-502,506.
24. 王浩, 王绍康, 邱兆成, 等. 户外鸽子生物机器人的设计及初步应用. 生物医学工程学杂志, 2022, 39(6): 1209-1217.
25. Esmaeilpour Z, Kronberg G, Reato D, et al. Temporal interference stimulation targets deep brain regions by modulating neural oscillations. Brain Stimul, 2021, 14(1): 55-65.
26. Guo W, He Y, Zhang W, et al. A novel non-invasive brain stimulation technique: “Temporally interfering electrical stimulation”. Front Neurosci, 2023, 17: 1092539.
27. Zhang Z, Lin B S, Wu C W G, et al. Designing and pilot testing a novel transcranial temporal interference stimulation device for neuromodulation. IEEE T Neur Sys Reh, 2022, 30: 1483-1493.

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