Distribution and Contamination Rate Analysis of Blood Culture in Children_West China Medical Journal

Authors：

 JIANGWen-qiang ,  LIHong-xia , LUOJun , DENGQiang , LIFen

Clinical Laboratory of Mianyang Central Hospital, Mianyang, Sichuan 621000, P. R. China;

Corresponding author：

LIHong-xia, Email: lihongxia1118@163.com

Keywords：

Automated blood culture system; Children; Blood culture; Bacteria; Contamination rate

DOI：

10.7507/1002-0179.20140214

Video：

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Objective To investigate the distribution of bacteria detected from blood culture of pediatric patients and to observe the blood culture contamination rate. Methods A total of 6 530 blood samples, collected from January 2011 to December 2012 were detected by BacT/Alert 3D automated blood culture system. We found out the contamination bacteria according to clinical data, laboratory data and microbiology knowledge. Results A total of 314 bacteria strains were isolated from 6 530 blood samples, and the positive rate was 4.8%, 228 of which were gram-positive bacteria. The isolates were mainly coagulase-negative staphylococci (43.9%), followed by Staphylococcus aureus (2.9%). In addition, 86 cases were gram-negative bacteria, the majority of which were Escherichia coli (9.6%), followed by Klebsiella pneumonia (8.3%). The overall blood culture contamination rate was 49.7% (156 bacteria were identified). The top two were coagulase-negative staphylococci (31.2%), followed by Bacillus sp. (6.4%). Conclusion The contamination rate is high in children's blood culture, and coagulase-negative staphylococci are the main bacteria. It's necessary to use clinical data and laboratory data to determine its clinical significance, and avoid unnecessary use of antibiotics.

Citation： JIANGWen-qiang, LIHong-xia, LUOJun, DENGQiang, LIFen. Distribution and Contamination Rate Analysis of Blood Culture in Children. West China Medical Journal, 2014, 29(4): 718-720. doi: 10.7507/1002-0179.20140214 Copy

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2.	Lee CC, Lin WJ, Shih HI, et al. Clinical significance of potential contaminants in blood cultures among patients in a medical center[J]. J Microbiol Immunol Infect, 2007, 40(5):438-444.
3.	Kristóf K, Kocsis E, Nagy K. Clinical microbiology of early-onset and late-onset neonatal sepsis, particularly among preterm babies[J]. Acta Microbiol Immunol Hung, 2009, 56(1):21-51.
4.	乔宁, 喻华. BacT/Alert240全自动血培养仪临床应用评价[J]. 实用医院临床杂志, 2009, 6(1):61-62.
5.	陶传敏, 温晓波, 邝玉, 等. BacT/Alert 240全自动血培养仪的临床应用及评价[J]. 华西医学, 2002, 17(3):366-368.
6.	曹慧玲, 李岷, 魏源华, 等. 血培养病原菌分布及污染菌判定的实验室检查[J]. 国际检验医学杂志, 2011, 32(18):2067-2068, 2071.
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8.	Richter SS, Beekmann SE, Croco JL, et al. Minimizing the workup of blood culture contaminants:implementation and evaluation of a laboratory-based algorithm[J]. J Clin Microbiol, 2002, 40(7):2437-2444.
9.	王进, 肖永红. 2006-2007年Mohnarin血流感染病原菌构成及耐药性[J]. 中华医院感染学杂志, 2008, 18(9):1238-1242.
10.	徐修礼, 杨春龙, 樊新, 等. 血培养标本中病原菌分布及其耐药性分析[J]. 中华医院感染学杂志, 2008, 18(10):1456-1459.
11.	孙宝君, 邹琳. 血培养阳性病原菌分布及变化趋势的临床分析[J]. 解放军医学杂志, 2005, 30(5):430-431.
12.	Favre B, Hugonnet S, Correa L, et al. Nosocomial bacteremia:clinical significance of a single blood culture positive for coagulase-negative staphylococci[J]. Infect Control Hosp Epidemiol, 2005, 26(8):697-702.
13.	李天娇, 钟海英, 郭红荔, 等. 新生儿血培养出凝固酶阴性葡萄球菌的分析[J]. 中国实验诊断学, 2010, 14(9):1451-1453.
14.	王辉, 张悦娴, 谢秀丽, 等. 血培养凝固酶阴性葡萄球菌阳性的临床意义[J]. 中国抗感染化疗杂志, 2001, 1(2):79-82.
15.	陈杏春. 血培养对新生儿败血症的诊断价值[J]. 广西医学, 2010, 32(8):970-972.
16.	王坚镪, 汤瑾, 庄亦晖, 等. 双套血培养对提高血流感染检出率和鉴别污染的评价[J]. 中国感染与化疗杂志, 2012, 12(6):440-442.
17.	毛美丽, 汪瑞忠. 血培养单、双侧采血阳性率比较及病原菌分布和耐药性分析[J]. 检验医学, 2009, 24(12):911-913.
18.	顾海彤, 黄艳飞, 孙宇峰, 等. 不同种类微生物血培养阳性报警时间的临床意义探讨[J]. 中国实验诊断学, 2011, 15(11):1882-1884.
19.	Roth A, Wiklund AE, Pålsson AS, et al. Reducing blood culture contamination by a simple informational intervention[J]. J Clin Microbiol, 2010, 48(12):4552-4558.

1. Yanai M, Uehara Y, Yagoshi M, et al. Interpretation of the results of blood culture and interventional role by clinical laboratory physicians[J]. Rinsho Byori, 2006, 54(10):1059-1065.
2. Lee CC, Lin WJ, Shih HI, et al. Clinical significance of potential contaminants in blood cultures among patients in a medical center[J]. J Microbiol Immunol Infect, 2007, 40(5):438-444.
3. Kristóf K, Kocsis E, Nagy K. Clinical microbiology of early-onset and late-onset neonatal sepsis, particularly among preterm babies[J]. Acta Microbiol Immunol Hung, 2009, 56(1):21-51.
4. 乔宁, 喻华. BacT/Alert240全自动血培养仪临床应用评价[J]. 实用医院临床杂志, 2009, 6(1):61-62.
5. 陶传敏, 温晓波, 邝玉, 等. BacT/Alert 240全自动血培养仪的临床应用及评价[J]. 华西医学, 2002, 17(3):366-368.
6. 曹慧玲, 李岷, 魏源华, 等. 血培养病原菌分布及污染菌判定的实验室检查[J]. 国际检验医学杂志, 2011, 32(18):2067-2068, 2071.
7. 张斗星, 张林, 李宗光, 等. 探讨血培养阳性结果在新生儿血流感染中的意义[J]. 安徽医学, 2012, 33(10):1290-1292.
8. Richter SS, Beekmann SE, Croco JL, et al. Minimizing the workup of blood culture contaminants:implementation and evaluation of a laboratory-based algorithm[J]. J Clin Microbiol, 2002, 40(7):2437-2444.
9. 王进, 肖永红. 2006-2007年Mohnarin血流感染病原菌构成及耐药性[J]. 中华医院感染学杂志, 2008, 18(9):1238-1242.
10. 徐修礼, 杨春龙, 樊新, 等. 血培养标本中病原菌分布及其耐药性分析[J]. 中华医院感染学杂志, 2008, 18(10):1456-1459.
11. 孙宝君, 邹琳. 血培养阳性病原菌分布及变化趋势的临床分析[J]. 解放军医学杂志, 2005, 30(5):430-431.
12. Favre B, Hugonnet S, Correa L, et al. Nosocomial bacteremia:clinical significance of a single blood culture positive for coagulase-negative staphylococci[J]. Infect Control Hosp Epidemiol, 2005, 26(8):697-702.
13. 李天娇, 钟海英, 郭红荔, 等. 新生儿血培养出凝固酶阴性葡萄球菌的分析[J]. 中国实验诊断学, 2010, 14(9):1451-1453.
14. 王辉, 张悦娴, 谢秀丽, 等. 血培养凝固酶阴性葡萄球菌阳性的临床意义[J]. 中国抗感染化疗杂志, 2001, 1(2):79-82.
15. 陈杏春. 血培养对新生儿败血症的诊断价值[J]. 广西医学, 2010, 32(8):970-972.
16. 王坚镪, 汤瑾, 庄亦晖, 等. 双套血培养对提高血流感染检出率和鉴别污染的评价[J]. 中国感染与化疗杂志, 2012, 12(6):440-442.
17. 毛美丽, 汪瑞忠. 血培养单、双侧采血阳性率比较及病原菌分布和耐药性分析[J]. 检验医学, 2009, 24(12):911-913.
18. 顾海彤, 黄艳飞, 孙宇峰, 等. 不同种类微生物血培养阳性报警时间的临床意义探讨[J]. 中国实验诊断学, 2011, 15(11):1882-1884.
19. Roth A, Wiklund AE, Pålsson AS, et al. Reducing blood culture contamination by a simple informational intervention[J]. J Clin Microbiol, 2010, 48(12):4552-4558.

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