Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats_West China Medical Journal

Authors：

 JiangHua ^1,2 , HeChengqi ^2,3 , OuYuping ² , YuanJulian ² , YangYang ² , SunZengchun ² , JiaXue ² ,  ZhouXue ¹

1. Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine;
2. Center of Rehabilitation Medicine, West China Hospital;Sichuan University, Chengdu, Sichuan 610041, P. R. China;
3. Institute of Disaster Management and Reconstruction, Sichuan University-The Hong Kong Polytechnic University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

ZhouXue, Email: zhouxue7239@163.com

Keywords：

Pilose antler blood wine; Osteoporosis; Bone mineral density; Immune function

DOI：

10.7507/1002-0179.20160076

Video：

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Objective To explore the effects of pilose antler blood wine on bone mineral density and T-lymphocyte subsets of ovariectomized female Sprague Dawley (SD) rats. Methods Sixty female SD rats were randomly divided into six groups:normal control group, osteoporosis model group, pure ethylalcohol group, high dose of pilose antler blood wine group, medium dose of pilose antler blood wine group and low dose of pilose antler blood wine group. Normal control group and osteoporosis model group were given saline with the dose of 9.1 g/(kg·d). Pure ethylalcohol group was given pure ethylalcohol with the dose of 9.1 g/(kg·d). The doses of pilose antler blood wine were 4.5, 9.1 and 13.6 g/(kg·d) respectively in low, medium and high dose of pilose antler blood wine group. After the feeding of pilose antler blood wine for 30 days, the bone mineral density and T-lymphocyte subsets of rats were assessed. Results The difference in bone mineral density among the three pilose antler blood wine groups was significant (P<0.05); the most obvious improvement of one mineral density was found in low and medium dose of pilose antler blood wine groups (P<0.05). The CD4⁺ T cells and CD4⁺/CD8⁺ significantly increased and CD8⁺ T cells proportion decreased significantly in pilose antler blood wine group compared with those in the osteoporosis model group (P<0.05). CD4⁺ T cells in medium dose group increased obviously compared with that in the high dose group (P<0.05). CD4⁺/CD8⁺ in low and medium dose groups obviously increased compared with that in high dose group (P<0.05). CD4⁺ T cells of medium dose group obviously increased compared with that in pure ethylalcohol group (P<0.05). CD8⁺ T cells in medium and low dose groups obviously decreased compared that in pure ethylalcohol group (P<0.05). CD4⁺/CD8⁺ in the three dose groups increased significantly compared with that in pure ethylalcohol group (P<0.05). Conclusion Tree different doses of pilose antler blood wine could prevent the osteoporosis and improve the immune function. The effects of medium and low dose of groups were more obvious.

Citation： JiangHua, HeChengqi, OuYuping, YuanJulian, YangYang, SunZengchun, JiaXue, ZhouXue. Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats. West China Medical Journal, 2016, 31(2): 283-286. doi: 10.7507/1002-0179.20160076 Copy

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23.	邱学敏, 王凌, 李大金.调节性细胞对破骨细胞的调控作用及其机制[J]. 现代免疫学,2013,33(1):71-73.

1. 何成奇. 骨质疏松症的康复治疗技术[M]. 北京:人民卫生出版社, 2008:1-19.
2. 杨永红, 何成奇, 杨霖, 等. 不同强度脉冲电磁场对去势大鼠血清雌二醇的影响[J]. 四川大学学报:医学版, 2008, 39(2):256-258.
3. 张月峰, 韩景献. 骨质疏松动物模型研究进展[J]. 动物医学进展, 2005, 26(3):8-11.
4. 杨建虹, 费聿荣, 曹毅, 等. 鹿茸血处理前后去势大鼠股骨的SRXRF分析[J]. 核技术, 2007, 30(5):407-410.
5. 高强, 杨扬, 孙增春, 等. 鹿茸血酒对势大鼠的升雌激素与抗氧化作用的初步研究[J].四川大学学报:医学版, 2014, 45(1):134-137.
6. 黄鑫, 孟国林, 刘建. 免疫因素与骨质疏松[J]. 中国骨质疏松杂志, 2010, 16(3):225-228.
7. 郭锦丽, 耿俊梅, 苏芙蓉, 等. 酒精性骨疾病发生机制的研究现状[J]. 实用骨科杂志, 2009, 15(2):111-114.
8. Holbrook T, Barrett-Connor E. A prospective study of alcohol consumption and bone mineral density[J]. BMJ, 1993, 306(6891):1506-1509.
9. Kim MJ, Shim MS, Kim MK, et al. Effect of chronic alcohol ingestion on bone mineral density in males without liver cirrhosis[J]. Korean J Intern Med, 2003, 18(3):174-180.
10. Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited[J]. FASEB J, 2008, 22(3):659-661.
11. 何成奇, 王维, 肖登, 等. 不同频率脉冲电磁场对去势大鼠血清雌二醇及股骨骨钙含量的影响[J]. 中国康复医学杂志, 2007, 22(4):303-305.
12. Abbott TA 3rd, Mucha L, Manfredonia D, et al. Efficient patient identification strategies for women with osteoporosis[J]. J Clin Densitom, 1999, 2(3):223-230.
13. van't Hof RJ, Ralston SH. Nitric oxide and bone[J]. Immunology, 2001, 103(3):255-161.
14. 丁桂芝, 李仁康, 李榕, 等. 从骨矿含量变化规律看肾主骨理论的科学性[J]. 湖北中医杂志, 1991, 13(2):27-29.
15. 李丽静, 王喜军, 王晓丽. 纳米三鹿方对骨质疏松大鼠骨密度及钙磷含量的影响[J]. 现代中医药, 2006, 26(3):54-56.
16. 国家药典委员会. 中华人民共和国药典[J]. 北京:化学工业出版社, 2009:79-82.
17. 齐艳萍. 鹿茸的化学成分及对肝损伤的修复作用[J]. 甘肃中医, 2010, 23(1):61-63.
18. 王守本, 孙德水, 周淑荣. 鹿血与鹿茸血的研究利用概况[J]. 特产研究, 1999(1):52-57.
19. Kishi S, Segawa Y, Yamaquchi M. Histomorphological confirmation of the preventive effect of beta-alanyl-L-histidinato zinc on bone loss in ovariectomized rats[J]. Biol Pharm Bull, 1994, 17(6):862-865.
20. Pacifici R. T cells:critical bone regulators in health and disease[J]. Bone, 2010, 47(3):461-471.
21. Nomura K, Kuroda S, Yoshikawa H, et al. Inflammatory osteoclast genesis can be induced by GM-CSF and activated under TNF immunity[J]. Biochem Biophs Res Commun, 2008, 367(4):881-887.
22. 董万超, 刘春华, 赵立波, 等. 马鹿茸、梅花鹿茸不同部位无机元素含量测定分析[J]. 特产研究, 2004, 26(3):32-36.
23. 邱学敏, 王凌, 李大金.调节性细胞对破骨细胞的调控作用及其机制[J]. 现代免疫学,2013,33(1):71-73.

West China Medical Journal

Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats

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