EXPERIMENTAL STUDY ON CD105+/CD166+ CELLS AND ITS CHONDROGENIC POTENTIAL IN EARLY OSTEOARTHRITIS CARTILAGE_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANG Min ¹ , Peter Chen ² , LIU Chao ¹ , HAO Yong ¹ , ZHANG Yuan ¹ , ZHANG Xia ¹

1Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, P.R.China;;
2Department of Bioengineering, University of California San Diego, USA, and Scripps Orthopeadic Research Institution, San Diego, CA, USA. Corresponding author: WANG Min, E-mail: 52solar@sina.cn;

Corresponding author：

Keywords：

Chondrocyte stem cells; Osteoarthritis; CD105; CD166; Rabbit

DOI：

10.7507/1002-1892.20130175

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Objective To study the variation of CD105+/CD166+ cells and its multilineage potential in early osteoarthritis (OA) cartilage so as to lay a foundation for cartilage repair and pathologic cartilage remodeling in arthritis. Methods The knee OA model was established in the right knee of 30 adult New Zealand rabbits (8-12 months old). The chondrocytes were harvested from normal cartilage of the left knee (group A), OA cartilage of the right knee at 2 weeks (group B), at 4 weeks (group C), and at 8 weeks (group D) after modeling, and BMSCs were used in group E for the expression of CD105 and CD166. The percentage of CD105+/CD166+ cells in each group was counted by flow cytometry, and CD105+/CD166+ cells were isolated and purified by magnetic-activated cell sorting. The expressions of CD105 and CD166 were observed in 5 groups by laser scanning confocal microscope. Chondrogenesis, osteogenesis, and adipogenesis were evaluated with Alcian blue cytochemistry and collagen type II immunohistochemistry, by detecting the deposition of calcium, and with oil red O staining, respectively. Results The percentage of CD105+/CD166+ cells in group A, B, C, and D was significantly lower than that in group E (P lt; 0.05); it was significantly higher in groups B, C, and D than in group A (P lt; 0.05), and in group D than in groups B and C (P lt; 0.05), but no significant difference was found between groups B and C (P gt; 0.05). Laser scanning confocal microscope results confirmed the expressions of CD105+ and CD166+ cells in groups A, B, C, D, and E, no obvious difference in expression was shown among 5 groups. At 1 week after chondrogenic induction, positive expressions of proteoglycan and collagen type II were observed in 5 groups, no obvious difference was noticed among 5 groups. At 2 weeks after osteogenic induction, calcium level in group E was significantly higher than that in groups A, B, C, and D (P lt; 0.05), but no significant different was found among groups A, B, C, and D (P gt; 0.05). At 4 weeks after adipogenic induction, there were more red lipid droplets in group E than in groups A, B, C, and D. Conclusion CD105+/CD166+ cells in early OA cartilage increase, which show chondrogenic differentiation potential.

Citation： WANG Min,Peter Chen,LIU Chao,HAO Yong,ZHANG Yuan,ZHANG Xia. EXPERIMENTAL STUDY ON CD105+/CD166+ CELLS AND ITS CHONDROGENIC POTENTIAL IN EARLY OSTEOARTHRITIS CARTILAGE. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(7): 793-799. doi: 10.7507/1002-1892.20130175 Copy

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13.	Grogan SP, Miyaki S, Asahara H, et al. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. Arthritis Res Ther, 2009, 11(3): R85.
14.	Dowthwaite GP, Bishop JC, Redman SN, et al. The surface of articular cartilage contains a progenitor cell population. J Cell Sci, 2004, 117(Pt 6): 889-897.
15.	Ridley AJ, Schwartz MA, Burridge K, et al. Cell migration: integrating signals from front to back. Science, 2003, 302(5651): 1704-1709.
16.	Kruegel J, Sadowski B, Miosge N. Nidogen-1 and nidogen-2 in healthy human cartilage and in late-stage osteoarthritis cartilage. Arthritis Rheum, 2008, 58(5): 1422-1432.
17.	Saito M, Sasho T, Yamaguchi S, et al. Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model. Osteoarthritis Cartilage, 2012, 20(12): 1574-1582.
18.	Heida NM, Muller JP, Cheng IF, et al. Effects of obesity and weight loss on the functional properties of early outgrowth endothelial progenitor cells. J Am Coll Cardiol, 2010, 55(4): 357-367.
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21.	Karlsson C, Stenhamre H, Sandstedt J, et al. Neither Notch1 expression nor cellular size correlate with mesenchymal stem cell properties of adult articular chondrocytes. Cells Tissues Organs, 2008, 187(4): 275-285.
22.	Williams R, Khan IM, Richardson K, et al. Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage. PLoS One, 2010, 5(10): e13246.
23.	Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of cultures of marrow derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol, 1998, 176(1): 57-66.

1. Caplan AI. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol, 2007, 213(2): 341-347.
2. Fickert S, Fiedler J, Brenner RE. Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers. Arthritis Res Ther, 2004, 6(5): R422-432.
3. Alsalameh S, Amin R, Gemba T, et al. Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage. Arthritis Rheum, 2004, 50(5): 1522-1532.
4. Pretzel D, Linss S, Rochler S, et al. Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. Arthritis Res Ther, 2011, 13(2): R64.
5. Koelling S, Miosge N. Stem cell therapy for cartilage regeneration in osteoarthritis. Expert Opin Biol Ther, 2009, 9(11): 1399-1405.
6. Koelling S, Miosge N. Sex differences of chondrogenic progenitor cells in late stages of osteoarthritis. Arthritis Rheum, 2010, 62(4): 1077-1087.
7. Koelling S, Kruegel J, Irmer M, et al. Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis. Cell Stem Cell, 2009, 4(4): 324-335.
8. Pickarski M, Hayami T, Zhuo Y, et al. Molecular changes in articular cartilage and subchondral bone in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis. BMC Musculoskelet Disord, 2011, 12: 197-212.
9. Johnstone B, Hering TM, Caplan AI, et al. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res, 1998, 238(1): 265-272.
10. Henrotin Y, Pesesse L, Sanchez C. Subchondral bone and osteoarthritis: biological and cellular aspects. Osteoporos Int, 2012, 23, Suppl 8: S847-851.
11. Bernstein P, Sperling I, Corbeil D, et al. Progenitor cells from cartilageno osteoarthritis-grade-specific differences in stem cell marker expression. Biotechnol Prog, 2013, 29(1): 206-212.
12. Jones BA, Pei M. Synovium-derived stem cells: a tissue-specific stem cell for cartilage engineering and regeneration. Tissue Eng Part B Rev, 2012, 18(4): 301-311.
13. Grogan SP, Miyaki S, Asahara H, et al. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. Arthritis Res Ther, 2009, 11(3): R85.
14. Dowthwaite GP, Bishop JC, Redman SN, et al. The surface of articular cartilage contains a progenitor cell population. J Cell Sci, 2004, 117(Pt 6): 889-897.
15. Ridley AJ, Schwartz MA, Burridge K, et al. Cell migration: integrating signals from front to back. Science, 2003, 302(5651): 1704-1709.
16. Kruegel J, Sadowski B, Miosge N. Nidogen-1 and nidogen-2 in healthy human cartilage and in late-stage osteoarthritis cartilage. Arthritis Rheum, 2008, 58(5): 1422-1432.
17. Saito M, Sasho T, Yamaguchi S, et al. Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model. Osteoarthritis Cartilage, 2012, 20(12): 1574-1582.
18. Heida NM, Muller JP, Cheng IF, et al. Effects of obesity and weight loss on the functional properties of early outgrowth endothelial progenitor cells. J Am Coll Cardiol, 2010, 55(4): 357-367.
19. Sandell LJ, Aigner T. Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritis. Artritis Res, 2001, 3(2): 107-113.
20. Kim HA, Blanco FJ. Cell death and apoptosis in ostearthritic cartilage. Curr Drug Targets, 2007, 8(2): 333-345.
21. Karlsson C, Stenhamre H, Sandstedt J, et al. Neither Notch1 expression nor cellular size correlate with mesenchymal stem cell properties of adult articular chondrocytes. Cells Tissues Organs, 2008, 187(4): 275-285.
22. Williams R, Khan IM, Richardson K, et al. Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage. PLoS One, 2010, 5(10): e13246.
23. Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of cultures of marrow derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol, 1998, 176(1): 57-66.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON CD105+/CD166+ CELLS AND ITS CHONDROGENIC POTENTIAL IN EARLY OSTEOARTHRITIS CARTILAGE

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