• 1. Department of General Surgery, People's Hospital of Yaan City, Yaan 625000, Sichuan Province, China;
  • 2. Department of Hepatobiliary Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China;
  • 3. Department of Hepatobiliary Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, China;
LIBo, Email: liboer2002@126.com
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Objective To investigate the effect ofγ-ray irradiation on echinococcus protoscoleces before abdominal cavity implantation in mice. Methods Echinococcus protoscoleces were cultured in vitro, and then divided into 5 groups, four groups receivedγ-ray irradiation with 10 Gy, 20 Gy, 40 Gy, and 80 Gy, respectively. The remaining control group was not irradiated. The protoscoleces were then implanted into the abdominal cavity of mice. Four months later, the incidence of echinococcosis was measured by detection of echinococcus antibody in the blood and then abdominal laparotomy was performed to isolate intraabdominal cysts, and the weight and structure of the echinococcus generated from the protoscoleces were measured and observed. Results After implantation of irradiated echinococcus protoscoleces in mice, the incidence of echinococcosis was 100% in the control group, 80.0% in the 10 Gy group, 33.3% in the 20 Gy group, 33.3% in the 40 Gy group, and 26.7% in the 80 Gy group. The echinococci had lower weight in the irradiated groups with 10 Gy (35.80 mg), with 20 Gy (0.00 mg), with 40 Gy (0.00 mg), and with 80 Gy (0.00 mg) as compared with the control group (157.80 mg). The echinococcus generated from the protoscoleces appeared calcification. Conclusions γ-ray irradiation could inhibit the formation of echinococcus granulosus generated from protoscoleces, damage the structure of echinococcus generated from the protoscoleces. Irradiation might prevent echinococcosis recurrence after surgical removal of hydatid cysts.

Citation: YUANQing, LIBo, JIANGShi-ping, LUOLan-lun, LIGeng, ZHAOQiang, ZHANGLi-yong. Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice In Vivo. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(2): 139-143. doi: 10.7507/1007-9424.20150039 Copy

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