Effects of Roux-en-Y Gastric Bypass on Intestinal Microbiota of Normal Sprague-Dawley Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIXiao-ke ^1,2 , QIUNian-cun ¹ , DUZhi-peng ¹ , ZHANGQin ¹ , RAOWen-sheng ¹ ,  SHANCheng-xiang ¹ ,  XUHui ²

1. Department of General Surgery, Affiliated Changzheng Hospital of The Second Military Medical University, Shanghai 200003, China;
2. Department of Oncology, No. 422 Hospital of PLA, Zhanjiang 524005, Guangdong Province, China;

Corresponding author：

SHANCheng-xiang, Email: chengxiangshan@hotmail.com; XUHui, Email: qium127@163.com

Keywords：

Roux-en-Y gastric bypass; Intestinal microbiota; Sprague-Dawley rats; Obesity

DOI：

10.7507/1007-9424.20150274

Video：

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Objective To investigate the effect of Roux-en-Y gastric bypass (RYGB) on the composition of intestinal microbiota among the biliopancreatic limb, the Roux limb, and the common channel in normal Sprague-Dawley (SD) rats. Methods Sixteen SD rats were randomly divided into sham surgery group (Sham group) and RYGB group, each group enrolled 8 rats. Rats in Sham group underwent sham surgery of end to end anastomosis in situ after cutting off the stomach and jejunum, and rats in RYGB group underwent RYGB. Then quantitative real-time PCR (RT-PCR) method was used to detect the expression of total bacteria, Bifidobacterium, Bacteroides, and Lactobacillus mRNA at biliopancreatic limb, the Roux limb, and the common channel. At last the comparison of mRNA in 4 kinds of bacteria was performed. Results Compared with Sham group, the weight of rats in RYGB group was lower at 8 weeks after surgery (P<0.01). RT-PCR results showed that, expression levels of total bacteria, Bifidobacterium, and Bacteroides mRNA at the Roux limb and the common channel in RYGB group were higher than corresponding site of rats in Sham group (P<0.05), but there was no significant difference at biliopancreatic limb between the 2 groups (P>0.05). Expression level of Lactobacillus mRNA at the Roux limb in RYGB group was higher than corresponding site of rats in Sham group (P<0.05), but there was no significant difference at biliopancreatic limb and the common channel between the 2 groups (P>0.05). Conclusion RYGB can significantly improve expression levels of the total bacteria, Bifidobacterium, and Bacteroides mRNA at Roux limb and the common channel, increase the level of Lactobacillus mRNA at Roux limb, while has no influence on biliopancreatic limb.

Citation： LIXiao-ke, QIUNian-cun, DUZhi-peng, ZHANGQin, RAOWen-sheng, SHANCheng-xiang, XUHui. Effects of Roux-en-Y Gastric Bypass on Intestinal Microbiota of Normal Sprague-Dawley Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(9): 1042-1046. doi: 10.7507/1007-9424.20150274 Copy

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14.	Liou AP, Paziuk M, Luevano JM Jr, et al. Conserved shifts in the gut microbiota due to gastric bypass reduce host weight and adiposity. Sci Transl Med, 2013, 5(178):178ra41.
15.	Clarke SF, Murphy EF, Nilaweera K, et al. The gut microbiota and its relationship to diet and obesity:new insights. Gut Microbes, 2012, 3(3):186-202.
16.	Jumpertz R, Le DS, Turnbaugh PJ, et al. Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans. Am J Clin Nutr, 2011, 94(1):58-65.
17.	Hansen F, Sangild PT, Vrang N, et al. L-cell distribution in the GI-tract of ZDF rats. Regul Pept, 2012, 177(8):S21-S22.
18.	王勇, 白洁, 匡立润, 等. Roux-en-Y胃旁路术对GK大鼠骨骼肌组织胰岛素抵抗影响的机制研究. 中国普外基础与临床杂志, 2014, 21(2):162-167.
19.	Walker AW, Duncan SH, Leitch EC, et al. pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Appl Environ Microbiol, 2005, 71(7):3692-3700.
20.	Dicksved J, Lindberg M, Rosenquist M, et al. Molecular characteriza-tion of the stomach microbiota in patients with gastric cancer and in controls. J Med Microbiol, 2009, 58(Pt 4):509-516.
21.	Islam KB, Fukiya S, Hagio M, et al. Bile acid is a host factor that regulates the composition of the cecal microbiota in rats. Gastroenterology, 2011, 141(5):1773-1781.
22.	周玉龙, 韩晓东, 狄建忠, 等. Roux-en-Y胃旁路术治疗2型糖尿病的研究进展. 中国普外基础与临床杂志, 2014, 21(2):246-250.

1. International Diabetes Federation. IDF diabetes atlas 6th edition revision. 2014. http://www.idf.org/diabetesatlas.
2. Xu Y, Wang L, He J, et al. Prevalence and control of diabetes in Chinese adults. JAMA, 2013, 310(9):948-958.
3. Colquitt JL, Pickett K, Loveman E, et al. Surgery for weight loss in adults. Cochrane Database Syst Rev, 2014, 8:CD003641.
4. Everard A, Lazarevic V, Derrien M, et al. Responses of gut micro-biota and glucose and lipid metabolism to prebiotics in genetic obese and diet-induced leptin-resistant mice. Diabetes, 2011, 60(11):2775-2786.
5. Aron-Wisnewsky J, Doré J, Clement K. The importance of the gut microbiota after bariatric surgery. Nat Rev Gastroenterol Hepatol, 2012, 9(10):590-598.
6. Li JV, Ashrafian H, Bueter M, et al. Metabolic surgery profoundly influences gut microbial-host metabolic cross-talk. Gut, 2011, 60(9):1214-1223.
7. Dewulf EM, Cani PD, Neyrinck AM, et al. Inulin-type fructans with prebiotic properties counteract GPR43 overexpression and PPAR_γ-related adipogenesis in the white adipose tissue of high-fat diet-fed mice. J Nutr Biochem, 2011, 22(8):712-722.
8. Turnbaugh PJ, Ley RE, Hamady M, et al. The human microbiome project. Nature, 2007, 449(7164):804-810.
9. Osto M, Abegg K, Bueter M, et al. Roux-en-Y gastric bypass surgery in rats alters gut microbiota profile along the intestine. Physiol Behav, 2013, 119(2):92-96.
10. Zhang H, Dibaise JK, Zuccolo A, et al. Human gut microbiota in obesity and after gastric bypass. Proc Natl Acad Sci U S A, 2009, 106(7):2365-2370.
11. Duncan SH, Lobley GE, Holtrop G, et al. Human colonic microbiota associated with diet, obesity and weight loss. Int J Obes (Lond), 2008, 32(11):1720-1724.
12. Santacruz A, Marcos A, Wärnberg J, et al. Interplay between weight loss and gut microbiota composition in overweight adolescents. Obesity (Silver Spring), 2009, 17(10):1906-1915.
13. Nadal I, Santacruz A, Marcos A, et al. Shifts in clostridia, bactero-ides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents. Int J Obes (Lond), 2009, 33(7):758-767.
14. Liou AP, Paziuk M, Luevano JM Jr, et al. Conserved shifts in the gut microbiota due to gastric bypass reduce host weight and adiposity. Sci Transl Med, 2013, 5(178):178ra41.
15. Clarke SF, Murphy EF, Nilaweera K, et al. The gut microbiota and its relationship to diet and obesity:new insights. Gut Microbes, 2012, 3(3):186-202.
16. Jumpertz R, Le DS, Turnbaugh PJ, et al. Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans. Am J Clin Nutr, 2011, 94(1):58-65.
17. Hansen F, Sangild PT, Vrang N, et al. L-cell distribution in the GI-tract of ZDF rats. Regul Pept, 2012, 177(8):S21-S22.
18. 王勇, 白洁, 匡立润, 等. Roux-en-Y胃旁路术对GK大鼠骨骼肌组织胰岛素抵抗影响的机制研究. 中国普外基础与临床杂志, 2014, 21(2):162-167.
19. Walker AW, Duncan SH, Leitch EC, et al. pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Appl Environ Microbiol, 2005, 71(7):3692-3700.
20. Dicksved J, Lindberg M, Rosenquist M, et al. Molecular characteriza-tion of the stomach microbiota in patients with gastric cancer and in controls. J Med Microbiol, 2009, 58(Pt 4):509-516.
21. Islam KB, Fukiya S, Hagio M, et al. Bile acid is a host factor that regulates the composition of the cecal microbiota in rats. Gastroenterology, 2011, 141(5):1773-1781.
22. 周玉龙, 韩晓东, 狄建忠, 等. Roux-en-Y胃旁路术治疗2型糖尿病的研究进展. 中国普外基础与临床杂志, 2014, 21(2):246-250.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Roux-en-Y Gastric Bypass on Intestinal Microbiota of Normal Sprague-Dawley Rats

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