Effects of Reduced Glutathione in Rats with Pulmonary Fibrosis_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

MaZhimin ¹ , LiXiaoling ² , WeiHuiqiang ¹ , HaoXiuling ¹ ,  GuoLiping ¹

1. Department of Respiratory Medicine, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 054000, China;
2. ;

Corresponding author：

GuoLiping, Email: wzb630601@126.com

Keywords：

Pulmonary fibrosis; Reduced glutathione; Rats

DOI：

10.7507/1671-6205.2016094

Video：

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Objective To explore the antioxidant effects of reduced glutathione on rat pulmonary fibrosis compared with traditional corticosteroid. Methods One-hundred and eight healthy SD rats were randomly divided into 6 groups,ie. a control group,a model group,a dexamethasone group,a low-dose glutathione group,a middle-dose glutathione group,and a high-dose glutathione group,with 18 rats in each group. The pulmonary fibrosis model was established by intratrachially instillation of bleomycin in all rats except the control group. The severity of lung fibrosis was evaluated by HE staining and Masson staining of collagen,and measurement of glutathione,hydroxyproline,superoxide dismutase (SOD),glutathion peroxidase (GSH-Px)in lung tissue homogenate by photocolorimetric method. Results On 7^th day and 14^th day after bleomycin instillation, the severity of alveolitis in the model group,the dexamethasone group,and three glutathione intervention groups was significantly reduced compared with the control group (P<0.05). On 28 day, the severity of lung fibrosis was significantly reduced in the dexamethasone group and three glutathione intervention groups compared with the model group (P<0.05). On 7^th day,lung glutathione content was significantly lower in the model group compared with the control group (P<0.05), significantly higher in the dexamethasone group and three glutathione intervention groups compared with the model group (P<0.05), significantly lower in the dexamethasone group and the low-dose glutathione group compared with the control group (P<0.05), and significantly higher in the high-dose glutathione group compared with the dexamethasone group (P<0.05). On 7^th,14^th,and 28^th day,the hydroxyproline content in the dexamethasone group and three glutathione intervention groups decreased significantly compared with the model group (P<0.05). On 14^th day,the hydroxyproline content in the middle-dose and high-dose glutathione groups was significantly lower than that in the dexamethasone group (P<0.05). SOD and GSH-Px were significantly reduced in the model group compared with the control group on all time points (P<0.05),but significantly increased after intervention by different doses of glutathione (P<0.05). Conclusion Reduced glutathione can significantly reduce the degree of pulmonary fibrosis in rats,but has no obvious advantage over dexamethasone.

Citation： MaZhimin, LiXiaoling, WeiHuiqiang, HaoXiuling, GuoLiping. Effects of Reduced Glutathione in Rats with Pulmonary Fibrosis. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 401-405. doi: 10.7507/1671-6205.2016094 Copy

1.	支开叶,康永,倪艳,等.肺纤方干预博莱霉素致肺纤维化大鼠的实验研究.中国实验方剂学杂志,2012,18:212-216.
2.	蔡健,顾振纶,蒋小岗,等.黄芩总黄酮对博莱霉素致大鼠肺纤维化的干预作用及其机制研究.中草药,2012,43:119-124.
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4.	刘瑞,李成刚,梁欣,等.博莱霉素诱导大鼠肺纤维化过程中羟脯氨酸及氧化应激指标的变化.医学争鸣,2005,25:2202-2205.
5.	唐仕芳,李华强,史源,等.还原型谷胱甘肽对新生鼠高氧肺纤维化早期干预的研究.重庆医学,2005,34:1190-1191.
6.	Todd NW,Luzina IG,Atamas SP. Molecular and cellular mechanisms of pulmonary fibrosis. Fibrogenesis Tissue Repair,2012,5:11.
7.	Griese M,Kappler M,Eismann C,et al. Inhalation treatment with glutathione in patients with cystic fibrosis. A randomized clinical trial. Am J Respir Crit Care Med,2013,188:83-89.
8.	Homma S,Azuma A,Taniguchi H,et al. Efficacy of inhaled N-acetylcysteine monotherapy in patients with early stage idiopathic pulmonary fibrosis. Respirology,2012,17:467-477.
9.	吕静. 还原型谷胱甘肽对博来霉素致大鼠肺纤维化的干预作用[学位论文].河北医科大学,2010.
10.	刘建,刘燕梅,王玉光. 黄芪甲苷对博莱霉素诱导小鼠肺纤维化的干预作用.北京中医药,2015,34:484-488.
11.	李柏颖,宋康,夏永良,等.虎杖对肺纤维化大鼠肺组织中SOD、GSH、MDA的影响. 浙江医学,2014,36:1385-1389.
12.	王笑英,韩玲,干泳华.桔梗总皂苷抗大鼠肺纤维化作用的机制.中国老年学杂志,2015,35:2957-2958.
13.	刘理静,钱红,张平.大黄素对肺纤维化大鼠的保护作用及部分机制研究.中国药理学通报,2015,31:266-272.
14.	支开叶,康永,倪艳,等.肺纤方对肺纤维化大鼠肺组织自由基损伤的影响.中国中医药信息杂志,2012,19:32-33.

1. 支开叶,康永,倪艳,等.肺纤方干预博莱霉素致肺纤维化大鼠的实验研究.中国实验方剂学杂志,2012,18:212-216.
2. 蔡健,顾振纶,蒋小岗,等.黄芩总黄酮对博莱霉素致大鼠肺纤维化的干预作用及其机制研究.中草药,2012,43:119-124.
3. 柴文戍,李永春,李艳勤,等.谷胱甘肽对大鼠肺纤维化模型的干预作用.中国医师杂志,2003,5:295-297.
4. 刘瑞,李成刚,梁欣,等.博莱霉素诱导大鼠肺纤维化过程中羟脯氨酸及氧化应激指标的变化.医学争鸣,2005,25:2202-2205.
5. 唐仕芳,李华强,史源,等.还原型谷胱甘肽对新生鼠高氧肺纤维化早期干预的研究.重庆医学,2005,34:1190-1191.
6. Todd NW,Luzina IG,Atamas SP. Molecular and cellular mechanisms of pulmonary fibrosis. Fibrogenesis Tissue Repair,2012,5:11.
7. Griese M,Kappler M,Eismann C,et al. Inhalation treatment with glutathione in patients with cystic fibrosis. A randomized clinical trial. Am J Respir Crit Care Med,2013,188:83-89.
8. Homma S,Azuma A,Taniguchi H,et al. Efficacy of inhaled N-acetylcysteine monotherapy in patients with early stage idiopathic pulmonary fibrosis. Respirology,2012,17:467-477.
9. 吕静. 还原型谷胱甘肽对博来霉素致大鼠肺纤维化的干预作用[学位论文].河北医科大学,2010.
10. 刘建,刘燕梅,王玉光. 黄芪甲苷对博莱霉素诱导小鼠肺纤维化的干预作用.北京中医药,2015,34:484-488.
11. 李柏颖,宋康,夏永良,等.虎杖对肺纤维化大鼠肺组织中SOD、GSH、MDA的影响. 浙江医学,2014,36:1385-1389.
12. 王笑英,韩玲,干泳华.桔梗总皂苷抗大鼠肺纤维化作用的机制.中国老年学杂志,2015,35:2957-2958.
13. 刘理静,钱红,张平.大黄素对肺纤维化大鼠的保护作用及部分机制研究.中国药理学通报,2015,31:266-272.
14. 支开叶,康永,倪艳,等.肺纤方对肺纤维化大鼠肺组织自由基损伤的影响.中国中医药信息杂志,2012,19:32-33.

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Chinese Journal of Respiratory and Critical Care Medicine

Effects of Reduced Glutathione in Rats with Pulmonary Fibrosis

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