The study of morphine mitochondrial toxicity impact on cat electroencephalogram_Journal of Epilepsy

Authors：

GUO Tao ,  WU Jiang , CHEN Yao , LIU Qianwei , LAN Yanqin

Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, China;

Corresponding author：

WU Jiang, Email: wujiang104@126.com

Keywords：

Morphine; Electroencephalogram; Ultramicro; Cat

DOI：

10.7507/2096-0247.20200003

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Objective To analyze the effect of mitochondrial ultrastructural changes caused by morphine toxicity on abnormal discharge of cat cerebral cortex, and to explore the possible mechanism of brain function damage caused by morphine dependence.Methods Twelve domestic cats were divided into control group (3 cats) and morphine exposed group (9 cats) according to the method of random number table. After the model was successfully established by the method of dose increasing, the changes of mitochondrial ultrastructure of cortical neurons were observed under the electron microscope.Results Electroencephalogram (EEG) monitoring in morphine exposed group showed that the cortical EEG was widely abnormal, physiological waves were reduced, and abnormal discharges were frequent. And the electron microscopy showed that the number, morphology, internal membrane structure and the inclusion body in the matrix of neurons changed in various aspects. The EEG and electron microscopy of the control group were normal.Conclusion Morphine can damage neurons in the cerebral cortex and lead to abnormal discharge, which is closely related to the ultrastructural changes of neuron mitochondria. The toxicity of morphine mitochondria can be the initial mechanism of energy metabolism dysfunction of brain cells and eventually lead to the disorder of brain electrophysiological function.

Citation： GUO Tao, WU Jiang, CHEN Yao, LIU Qianwei, LAN Yanqin. The study of morphine mitochondrial toxicity impact on cat electroencephalogram. Journal of Epilepsy, 2020, 6(1): 12-15. doi: 10.7507/2096-0247.20200003 Copy

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1. 赵美顺, 陈敬荣, 杨红. β淀粉样蛋白神经毒性与线粒体功能异常研究进展. 广东药学院学报, 2012, 28(6): 689-692.
2. Maldonado R, Negus S, Koob GF. Precipitation of morphine withdrawal syndrome in rats by administration of mu-, delta-and kappa-selective opioid antagonists. Neuropharmacology, 1992, 21(12): 1231-1241.
3. 郭韬, 杜亚丽, 张素芳, 等. 吗啡急性成瘾猫模型的建立. 脑与神经疾病杂志, 2008, 16(6): 711-712.
4. 朱筑霞, 吴泽江, 王旭东, 等. 胚胎期和生长期子代大鼠砷暴露对脑细胞线粒体功能和超微结构的影响. 环境与健康杂志, 2011, 6(28): 488-490.
5. Bazil JN, Dash RK. A minimal model for the mitochondrialrapid mode of Ca²⁺ uptake mechanism. PLoS One, 2011, 6(6): 213-224.
6. Pan S, Ryu SY, Sheu SS. Distinctive characteristics andfunctions of multiple mitochondrial Ca²⁺ influx mechanisms. Sci China Life Sci, 2011, 54(8): 763-769.

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