Effects of Bone Morphogenetic Protein-2 on Expression of DLX5 of the Neural Stem Cells of Anterior Subventricular Zone_West China Medical Journal

Authors：

LIU Shenghua ,  ZHOU Zheng , LIU Jun , YIN Jinbo

Department of Neurosurgery, Xinqiao Hospital, the Third Millitary Medical University, Chongqing 400037, P. R. China;

Corresponding author：

ZHOU Zheng, Email: lg65036@126.com

Keywords：

神经干细胞; 室管膜前下区; DLX5; 骨形成蛋白-2; Noggin

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【摘要】　目的　探讨骨形成蛋白-2（bone morphogenetic protein-2，BMP-2）对室管膜前下区（anterior subventricular zone，SVZa）神经干细胞DLX5表达的影响。　方法　体外培养SVZa神经干细胞，用BMP-2及其拮抗剂Noggin诱导SVZa神经干细胞，分别用免疫荧光染色和逆转录-聚合酶链反应（RT-PCR）检测DLX5表达变化。　结果　 BMP-2组SVZa神经干细胞DLX5蛋白表达和DLX5mRNA表达水平明显高于对照组（P lt;0.05），且该效应能被其拮抗剂Noggin特异性地抑制。　结论　 BMP-2是DLX5上游调节基因，可促进SVZa神经干细胞DLX5的表达。
【Abstract】　Objective　 To investigate the effect of bone morphogenetic protein-2 (BMP-2)on expression of DLX5 of neural stem cells in anterior subventricular zone (SVZa).　Methods　 The neural stem cells of SVZa were separated and cultured in vitro, which were induced by BMP-2 and Noggin.Immunofluorescence staining and RT-PCR were employed to assay the expression of DLX5.　Results　 The percentages of expression of DLX5 protein and DLX5 mRNA in BMP-2 group were much higher than those in the control group (P lt;0.05). And this induction could be specifically blocked by Noggin.　Conclusion　 BMP-2 is an upstream gene of DLX5; BMP-2 can promote the expression of DLX5 of the neural stem cells of SVZa.

Citation： LIU Shenghua,ZHOU Zheng,LIU Jun,YIN Jinbo. Effects of Bone Morphogenetic Protein-2 on Expression of DLX5 of the Neural Stem Cells of Anterior Subventricular Zone. West China Medical Journal, 2010, 25(12): 2177-2180. doi: Copy

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9.	张治元, 杨辉, 刘仕勇, 等. BMP-2在SVZa神经干细胞向神经元分化的作用[J]. 第三军医大学学报, 2003, 25(20): 1788-1791.
10.	陈锦华, 杨辉, 尹昌林, 等. BMP2在SVZa神经干细胞向GABA能神经元分化中的调控作用[J]. 第三军医大学学报, 2007, 29(8): 699-701.
11.	辛志成, 周政, 杨辉, 等. SVZa 神经干细胞诱导分化为多巴胺能神经元的实验研究[J]. 重庆医学, 2008, 37(5): 481-483.
12.	Panganiban G, Rubenstein JL. Developmental functions of the distal-less/DLX homeobox genes[J]. Development, 2002, 129(19): 4371-4386.
13.	Mabie PC, Mehler MF, Kessler JA, et al. Multiple roles of bone morphogenetic protein signaling in the regulation of cortical cell number and phenotype[J]. J Neurosci, 1999, 19 (16): 7077-7088.

1. 　Perera M, Merlo GR, Verardo S, et al. Defective neuronogenesis in the absence of DLX5[J]. Mol Cell Neruosci, 2004, 25(1): 153-161.
2. 　Levi G, Puche AC, Mantero S, et al. The DLX5 homeodomain gene is essential for olfactory development and connectivity in the mouse[J]. Mol Cell Neruosci, 2003, 22(4): 530-543.
3. 　Long JE, Garel S, Depew MJ, et al. DLX5 regulates development of peripheral and central components of the olfactory system[J]. J Neurosci, 2003, 23(2): 568-578.
4. 　Mehler MF, Mabie PC, Zhang D, et al. Bone morphogenetic proteins in the nervous system[J]. Trends Neurosci, 1997, 20(7): 309-317.
5. 　Stull ND, Jung JW, Iacovitti L. Induction of a dopaminergic phenotype in cultured striatal neurons by bone morphogenetic proteins[J]. Brain Res Dev Brain Res, 2001, 130(1): 91-98.
6. 　Bronner-Fraser M. Molecular analysis of neural crest formation[J]. J Physiol Paris, 2002, 96(1-2): 3-8.
7. 　Gomes WA, Mehler MF, Kessler JA. Transgenic overexpression of BMP4 increases astroglial and decreases oligodendroglial lineage commitment[J]. Dev Biol, 2003, 255(1): 164-177.
8. 　Harris SE, Guo DY, Harris MA, et al. Transcriptional regulation of BMP-2 activated genes in osteoblasts using gene expression microarray analysis: role of DLX2 and DLX5 transcription factors[J]. Front Biosci, 2003, 8(4): s1249-s1265.
9. 　张治元, 杨辉, 刘仕勇, 等. BMP-2在SVZa神经干细胞向神经元分化的作用[J]. 第三军医大学学报, 2003, 25(20): 1788-1791.
10. 　陈锦华, 杨辉, 尹昌林, 等. BMP2在SVZa神经干细胞向GABA能神经元分化中的调控作用[J]. 第三军医大学学报, 2007, 29(8): 699-701.
11. 　辛志成, 周政, 杨辉, 等. SVZa 神经干细胞诱导分化为多巴胺能神经元的实验研究[J]. 重庆医学, 2008, 37(5): 481-483.
12. 　Panganiban G, Rubenstein JL. Developmental functions of the distal-less/DLX homeobox genes[J]. Development, 2002, 129(19): 4371-4386.
13. 　Mabie PC, Mehler MF, Kessler JA, et al. Multiple roles of bone morphogenetic protein signaling in the regulation of cortical cell number and phenotype[J]. J Neurosci, 1999, 19 (16): 7077-7088.

West China Medical Journal

Effects of Bone Morphogenetic Protein-2 on Expression of DLX5 of the Neural Stem Cells of Anterior Subventricular Zone

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