目的:探讨Livin基因在人胆管癌组织及胆管癌细胞系中的表达情况及其与胆管癌发
生发展之间的关系。方法:采用免疫组织化学技术（SP法）检测Livin基因蛋白在45例人胆管
癌标本及及40例癌旁胆管组织、20例正常胆管组织标本中的表达；同时采用RTPCR法及SP
法检测了Livin基因mRNA和蛋白在人胆管癌细胞系QBC939及非肿瘤细胞系HT1080中表达。
结果:Livin在胆管癌组织中表达阳性率为57.8%，而癌旁胆管组织、非癌胆管组织中未能检
测到Livin表达。Livin表达与性别、年龄、肿瘤大小及肿瘤分化程度无关。在有淋巴结转组
中，Livin阳性表达率（70.4%）明显高于无淋巴结转移组（38.9%）。在人胆管癌细胞QBC939
中，Livin mRNA及蛋白均特异性表达，而非肿瘤细胞系HT1080未见Livin表达。结论:Livin
基因在人类胆管癌组织和细胞系中选择性高表达，其可能与胆管癌发生、发展及预后密切相
关。
Citation：
TANG Zhihua,CHEN Jisheng,YE Lin,YU Jiexiong. Expression and Significance of Livin in Human Cholangiocarcinoma Tissues and Cell Line. West China Medical Journal, 2009, 24(1): 67-69. doi:
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Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
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Kasof GM，Gomes BC.Livin，a novel inhibitor of apoptosis protein family member[J].J Biol Chem，2001，276（5）:3238-3246..
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Isa T，Tomita S，Nakaehi A，et al.Analysis of microsatellite instability，Kras gene mutation and P53 protein over expression intrahepatic cholangiocarcinoma[J]. Hepatogastroenterology，2002，49:604-608..
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Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
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Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.
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- 1. Kasof GM，Gomes BC.Livin，a novel inhibitor of apoptosis protein family member[J].J Biol Chem，2001，276（5）:3238-3246..
- 2. Gazzaniga P，Gradilone A，Giuliani L，et al.Expression and prognostic significance of livin，survivin and other apoptosis related genes in the progression of superficial bladder cancer[J].Ann Oncol，2003，14（1）:85-90..
- 3. Yagihashi A，Ohmura T，Asanuma K，et al.Detection of autoantibodies to survivin and livin in sera frompatients with breast cancer[J].Clin Chim Acta，2005，362（12）:125-130..
- 4. Burke EC，Jamagin WR，Hoehwald SN，et al.Hilar cholangiocarcinoma:patterns of spread，the importance of hepatic resection for curative operation，and a presurgical clinical staging system[J].Ann Surg，1998，228:385一394..
- 5. Isa T，Tomita S，Nakaehi A，et al.Analysis of microsatellite instability，Kras gene mutation and P53 protein over expression intrahepatic cholangiocarcinoma[J]. Hepatogastroenterology，2002，49:604-608..
- 6. Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
- 7. Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.
