Effect of Mycophenolate Mofetil Versus Cyclosporine A on Cytoprotective Genes Expression in Rat Kidney Allografts Undergoing Chronic Allograft Nephropathy_West China Medical Journal

Authors：

1.Department of Urology，West China Hospital，Sichuan University，Chengdu 610041;2.Department of Urology，People’s Hospital of Leshan，Leshan Sichuan 614000，China;

Corresponding author：

Keywords：

慢性移植肾病; 细胞保护基因; 吗替麦考酚酯; A20

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目的：研究吗替麦考酚酯（MMF）和环孢素A（CsA）对大鼠慢性移植肾病中细胞保护基因A20、HO-1、Bcl-2和Bcl-XL表达的影响。方法：分别采用雄性SD大鼠和Wistar大鼠作为供受体建立强化缺血/再灌注损伤慢性移植肾病模型。对受者依据所采用的免疫抑制剂方案分组：对照组、CsA组、MMF组。在移植术后的相同时间点处死大鼠，切取移植肾脏。采用荧光定量RTPCR和免疫组织化学方法检测A20、HO-1、Bcl2和BclXL的表达及其在移植肾中的定位情况。结果：在大鼠慢性移植肾病模型中，能够检测到所有上述四种细胞保护基因的表达。A20主要表达于血管内皮细胞和浸润之淋巴细胞。HO-1主要表达于浸润之淋巴细胞，但在肾小管上皮细胞也有表达。Bcl2和BclXL则主要表达于肾小管上皮细胞。MMF组A20的表达显著高于CsA组和对照组（P lt;0.01）。HO1，Bcl2和BclXL的表达在MMF组和CsA组间无显著性差异（P gt;0.05）。结论：MMF对A20表达的增强可能与MMF减轻大鼠慢性移植肾病病变的机制有关。

Citation： TENG Donghai,LU Yiping,ZHANG Yangang,et al.. Effect of Mycophenolate Mofetil Versus Cyclosporine A on Cytoprotective Genes Expression in Rat Kidney Allografts Undergoing Chronic Allograft Nephropathy. West China Medical Journal, 2009, 24(1): 135-137. doi: Copy

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1. Liu YH.Epithelial to mesenchymal transition in renal fibrogeneis：pathologic significance，molecular mechanism，and therapeutic intervention[J].J Am Soc Nephrol，2004，15（1）：1-12..
2. Kunter U，Floege J，Jurgensonn SA，et al.Expression of A20 in the vessel wall of ratkidney allografts correlates with protection from transplant arteriosclerosis[J].Transplantation，2003，75（1）：3-9..
3. Cooper JT，Stroka DM，Brostjan C，et al.A20 blocks endothelial cell activation through a NFkappaBdependent mechanism[J].J Biol Chem，1996，271（30）：18068-18073..
4. Soares MP，Lin Y，Anrather J，et al.Expression of heme oxygenase1 can determine cardiac xenograft survival[J].Nat Med，1998，4（9）：1073-1077..
5. Badrichani AZ，Stroka DM，Bilbao G，et al.Bcl2 and BclXL serve an antiinflammatory function in endothelial cells through inhibition of NFκB[J].J Clin Invest，1999，103（4）：543-553..
6. Dixit VM，Green S，Sarma V，et al.Tumor necrosis factorα induction of novel gene products in human endothelial cells including a macrophagespecific chemotaxin[J].J Biol Chem，1990，265（5）：2973-2978..
7. Lee EG，Boone DL，Chai S，et al.Failure to regulate TNFinduced NFκ B and cell death responses in A20deficient mice[J].Science，2000，289（5488）：2350-2354..
8. He KL，Ting AT.A20 inhibits tumor necrosis factor（TNF）αinduced apoptosis by disrupting recruitment of TRADD and RIP to the TNF receptor 1 complex in Jurkat T cells[J].Mol Cell Biol，2002，22（17）：6034-6045..
9. Gao R，Lu YP，Xin YP，et al.The effects of different immunosuppresants on CAN by affecting the TGFβ and SMADs signal pathway[J].Transplantation，2006，78（2）Suppl 1：609.

West China Medical Journal

Effect of Mycophenolate Mofetil Versus Cyclosporine A on Cytoprotective Genes Expression in Rat Kidney Allografts Undergoing Chronic Allograft Nephropathy

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