Effect of Recombinant Tissue Factor Pathway Inhibitor 2 Gene on The Invasion of Human Pancreatic Cancer in Vitro and in Vivo_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TANG Zhigang , SUN Zhenyang , HU Hejie , XU Geliang , CHEN Jiong , LI Jiansheng

Department of General Surgery , Anhui Province Hospital , Hefei 230001, China;

Corresponding author：

Keywords：

Pancreatic cancer, 　　Neoplasm metastasis, 　　Neoplasm invasiveness, 　　Tissue factor pathway inhibitor 2

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Objective 　To investigate the invasion ability of Panc-1 cells in vivo and in vitro af ter being t ransfected with tissue factor pathway inhibitor 2 gene ( TFPI-2) . Methods 　The expression vector pEGFP-C1-TFPI-2 was transfected into human pancreatic cancer line Panc-1 cells by using liposome. TFPI-2 mRNA and protein of transfected and nontransfected cells were detected by reverse t ranscription-polymerase chain reaction (RT-PCR) and Western blot respectively. The tumor cells invasive behavior of t ransfected ( Panc-1-TFPI-2) and nontransfected ( Panc-1-V and Panc-1-P) cells were assessed in vitro through Boyden Chamber method. The transfected and nontransfected cells were implanted into nude mice to observe it s growth and metastasis in vivo. Results 　Expressions of mRNA and protein of TFPI-2 were confirmed in transfected cells. Af ter TFPI-2 t ransfection , the number of Panc-1-TFPI-2 , Panc-1-V and Panc-1-P cells passing through membrane of Boyden Chamber were 24. 4 ±3. 5 ,61. 3 ±4. 1 and 60. 2 ±3. 9 , respectively. The number of TFPI-2-expressing cells to t raverse a Matrigel-coated membrane was obviously decreased compared with that of non-expressing cells , the invasion ability was lower than that before transfection in vitro. The subcutaneous tumor volume of the Panc-1-TFPI-2 group was (438. 0 ±69. 8) mm3 , the Panc-1-V group was (852. 0 ±102. 9) mm3 and the Panc-1-P group was (831. 0 ±78. 1) mm3 , P lt; 0. 05. The metastasis to liver and lung and muscular invasion occurred in the Panc-1-V group and the Panc-1-P group. There were no muscular invasion and metastatic lesions in the Panc-1-
TFPI-2 group. Conclusion 　TFPI-2 gene expression may obviously inhibit the invasion ability of pancreatic cancer cells in vitro and in vivo , which provides an experimental basis for the treatment of human pancreatic cancer by gene therapy.

Citation： TANG Zhigang,SUN Zhenyang,HU Hejie,XU Geliang,CHEN Jiong,LI Jiansheng. Effect of Recombinant Tissue Factor Pathway Inhibitor 2 Gene on The Invasion of Human Pancreatic Cancer in Vitro and in Vivo. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(1): 46-50. doi: Copy

1.	Wong CM,Ng YL,Lee JM,et al.Tissue factor pathway inhibitor-2 as a frequently silenced tumor suppressor gene in hepatocellular carcinoma[J].Hepatology,2007; 45(5)∶1129.
2.	Nobeyama Y,Okochi-Takada E,Furuta J,et al.Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas[J].Int J Cancer,2007; 121(2)∶301.
3.	Torres-Collado AX,Kisiel W,Iruela-Arispe ML,et al.ADAMTS1 interacts with,cleaves,and modifies the extracellular location of the matrix inhibitor tissue factor pathway inhibitor-2[J].J Biol Chem,2006; 281(26)∶17827.
4.	Hirata M,Sato T,Tsumagari M,et al.Discoordinate regulation of expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases-3 in bovine endometrial stromal cells on type-Ⅰ collagen gel[J].Biol Pharm Bull,2003; 26(7)∶1013.
5.	Siegling S,Thyzel E,Brinkmann T,et al.Genetic variations in the human TFPI-2 gene and its promoter region by denaturing high performance liquid chromatography[J].Thromb Res,2004; 113(5)∶341.
6.	Chen XY,Li JS,Ma J,et al.Potential role of novel hepatocellular carcinoma-associated gene IDD 01 in promoting tumorigenesis of HepG2 cell line[J].Chin Med J (Engl),2006; 119(20)∶1709.
7.	Tanaka Y,Utsumi J,Matsui M,et al.Purification,molecular cloning,and expression of a novel growth-promoting factor for retinal pigment epithelial cells,REF-1/TFPI-2[J].Invest Ophthalmol Vis Sci,2004; 45(1)∶245.
8.	Jiang P,Watanabe H,Okada G,et al.Diagnostic utility of aberrant methylation of tissue factor pathway inhibitor 2 in pure pancreatic juice for pancreatic carcinoma[J].Cancer Sci,2006; 97(11)∶1267.
9.	Neaud V,Hisaka T,Monvoisin A,et al.Paradoxical pro-invasive effect of the serine proteinase inhibitor tissue factor pathway inhibitor-2 on human hepatocellular carcinoma cells[J].J Biol Chem,2000; 275(45)∶35565.
10.	Blindt R,Vogt F,Lamby D,et al.Characterization of differential gene expression in quiescent and invasive human arterial smooth muscle cells[J].J Vasc Res,2002; 39(4)∶340.
11.	Jin M,Udagawa K,Miyagi E,et al.Expression of serine proteinase inhibitor PP5/TFPI-2/MSPI decreases the invasive potential of human choriocarcinoma cells in vitro and in vivo[J].Gynecol Oncol,2001; 83(2)∶325.
12.	Kong D,Ma D,Bai H,et al.Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2[J].Biochem Biophys Res Commun,2004; 324(4)∶1179.
13.	Konduri SD,Tasiou A,Chandrasekar N,et al.Role of tissue factor pathway inhibitor-2 (TFPI-2) in amelanotic melanoma (C-32) invasion[J].Clin Exp Metastasis,2000; 18(4)∶303.
14.	Ivanciu L,Gerard RD,Tang H,et al.Adenovirus-mediated expression of tissue factor pathway inhibitor-2 inhibits endothelial cell migration and angiogenesis[J].Arterioscler Thromb Vasc Biol,2007; 27(2)∶310.
15.	Iino M,Foster DC,Kisiel W.Quantification and characterization of human endothelial cell-derived tissue factor pathway inhibitor-2[J].Arterioscler Thromb Vasc Biol,1998; 18(1)∶40.
16.	Rao CN,Reddy P,Reeder DJ,et al.Prokaryotic expression,purification,and reconstitution of biological activities (Antiprotease,antitumor,and heparin-binding) for tissue factor pathway inhibitor-2[J].Biochem Biophys Res Commun,2000; 276(3)∶1286.
17.	Yanamandra N,Kondraganti S,Gondi CS,et al.Recombinant adeno-associated virus (rAAV) expressing TFPI-2 inhibits invasion,angiogenesis and tumor growth in a human glioblastoma cell line[J].Int J Cancer,2005; 115(6)∶998.
18.	Chand HS,Du X,Ma D,et al.The effect of human tissue factor pathway inhibitor-2 on the growth and metastasis of fibrosarcoma tumors in athymic mice[J].Blood,2004; 103(3)∶1069.
19.	Kondraganti S,Gondi CS,Gujrati M,et al.Restoration of tissue factor pathway inhibitor inhibits invasion and tumor growth in vitro and in vivo in a malignant meningioma cell line[J].Int J Oncol,2006; 29(1)∶25.
20.	Sun Y,Xie M,Liu M,et al.Growth suppression of human laryngeal squamous cell carcinoma by adenovirus-mediated tissue factor pathway inhibitor gene 2[J].Laryngoscope,2006; 116(4)∶596.

1. Wong CM,Ng YL,Lee JM,et al.Tissue factor pathway inhibitor-2 as a frequently silenced tumor suppressor gene in hepatocellular carcinoma[J].Hepatology,2007; 45(5)∶1129.
2. Nobeyama Y,Okochi-Takada E,Furuta J,et al.Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas[J].Int J Cancer,2007; 121(2)∶301.
3. Torres-Collado AX,Kisiel W,Iruela-Arispe ML,et al.ADAMTS1 interacts with,cleaves,and modifies the extracellular location of the matrix inhibitor tissue factor pathway inhibitor-2[J].J Biol Chem,2006; 281(26)∶17827.
4. Hirata M,Sato T,Tsumagari M,et al.Discoordinate regulation of expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases-3 in bovine endometrial stromal cells on type-Ⅰ collagen gel[J].Biol Pharm Bull,2003; 26(7)∶1013.
5. Siegling S,Thyzel E,Brinkmann T,et al.Genetic variations in the human TFPI-2 gene and its promoter region by denaturing high performance liquid chromatography[J].Thromb Res,2004; 113(5)∶341.
6. Chen XY,Li JS,Ma J,et al.Potential role of novel hepatocellular carcinoma-associated gene IDD 01 in promoting tumorigenesis of HepG2 cell line[J].Chin Med J (Engl),2006; 119(20)∶1709.
7. Tanaka Y,Utsumi J,Matsui M,et al.Purification,molecular cloning,and expression of a novel growth-promoting factor for retinal pigment epithelial cells,REF-1/TFPI-2[J].Invest Ophthalmol Vis Sci,2004; 45(1)∶245.
8. Jiang P,Watanabe H,Okada G,et al.Diagnostic utility of aberrant methylation of tissue factor pathway inhibitor 2 in pure pancreatic juice for pancreatic carcinoma[J].Cancer Sci,2006; 97(11)∶1267.
9. Neaud V,Hisaka T,Monvoisin A,et al.Paradoxical pro-invasive effect of the serine proteinase inhibitor tissue factor pathway inhibitor-2 on human hepatocellular carcinoma cells[J].J Biol Chem,2000; 275(45)∶35565.
10. Blindt R,Vogt F,Lamby D,et al.Characterization of differential gene expression in quiescent and invasive human arterial smooth muscle cells[J].J Vasc Res,2002; 39(4)∶340.
11. Jin M,Udagawa K,Miyagi E,et al.Expression of serine proteinase inhibitor PP5/TFPI-2/MSPI decreases the invasive potential of human choriocarcinoma cells in vitro and in vivo[J].Gynecol Oncol,2001; 83(2)∶325.
12. Kong D,Ma D,Bai H,et al.Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2[J].Biochem Biophys Res Commun,2004; 324(4)∶1179.
13. Konduri SD,Tasiou A,Chandrasekar N,et al.Role of tissue factor pathway inhibitor-2 (TFPI-2) in amelanotic melanoma (C-32) invasion[J].Clin Exp Metastasis,2000; 18(4)∶303.
14. Ivanciu L,Gerard RD,Tang H,et al.Adenovirus-mediated expression of tissue factor pathway inhibitor-2 inhibits endothelial cell migration and angiogenesis[J].Arterioscler Thromb Vasc Biol,2007; 27(2)∶310.
15. Iino M,Foster DC,Kisiel W.Quantification and characterization of human endothelial cell-derived tissue factor pathway inhibitor-2[J].Arterioscler Thromb Vasc Biol,1998; 18(1)∶40.
16. Rao CN,Reddy P,Reeder DJ,et al.Prokaryotic expression,purification,and reconstitution of biological activities (Antiprotease,antitumor,and heparin-binding) for tissue factor pathway inhibitor-2[J].Biochem Biophys Res Commun,2000; 276(3)∶1286.
17. Yanamandra N,Kondraganti S,Gondi CS,et al.Recombinant adeno-associated virus (rAAV) expressing TFPI-2 inhibits invasion,angiogenesis and tumor growth in a human glioblastoma cell line[J].Int J Cancer,2005; 115(6)∶998.
18. Chand HS,Du X,Ma D,et al.The effect of human tissue factor pathway inhibitor-2 on the growth and metastasis of fibrosarcoma tumors in athymic mice[J].Blood,2004; 103(3)∶1069.
19. Kondraganti S,Gondi CS,Gujrati M,et al.Restoration of tissue factor pathway inhibitor inhibits invasion and tumor growth in vitro and in vivo in a malignant meningioma cell line[J].Int J Oncol,2006; 29(1)∶25.
20. Sun Y,Xie M,Liu M,et al.Growth suppression of human laryngeal squamous cell carcinoma by adenovirus-mediated tissue factor pathway inhibitor gene 2[J].Laryngoscope,2006; 116(4)∶596.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Recombinant Tissue Factor Pathway Inhibitor 2 Gene on The Invasion of Human Pancreatic Cancer in Vitro and in Vivo

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