Objective To establish a new method for detection of β-glucuronidase (β-G) mRNA in human liver and kidney tissues.
Methods β-G mRNA expression was detected by reverse transcription polymerase chain reaction(RT-PCR) in 10 cases of normal liver tissues, 10 cases of normal kidney tissues and 8 cases of hepatocellular carcinoma tissues.
Results The expand products of β-G mRNA were expressed in liver and kidney tissues with similar size of 422 bp. The expression contents of β-G mRNA in liver and kidney normal tissues were different (1.71±0.32 vs 1.83±0.22) but without statistical significance (P gt;0.05). However, the expression content of β-G mRNA in hepatocellular carcinoma tissues was 3.88±0.86, which was significantly higher (P<0.01) than that in normal liver tissues.
Conclusion β-G mRNA determination is feasible by checking β-G gene alignment in gene bank and designing draw matter in the tissue of liver and kidney. It may be very significant to explore the change of β-G mRNA in various tissues in studying of molecular mechanism.
Citation: YANG Bo,ZHANG Hong,LI Wei,ZHOU Wenping.. Detection and Significance of β-Glucuronidase mRNA in Human Liver and Kidney Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 652-655. doi: Copy
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