Effects of Inhibit Extracellular-Signal Regulated Kinase 1/2 Pathway on Apoptosis of Hepatoma Carcinoma Cell SMMC-7721_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SHENG Wei ¹ , WANG Kang ¹ , PING Gaofeng ¹ , LI Xuqi ¹ , WEI Guangbing ¹ , HUANG Chen ²

Department of General Surgery, The First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061, China;

Corresponding author：

Keywords：

Extracellular-signal regulated kinase; Hepatoma carcinoma cell line; Proliferation; Apoptosis

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Objective 　 To observe the effects of extracellular-signal regulated kinase (ERK) 1/2 inhibitor U0126 on hepatoma carcinoma cell proliferation and apoptosis.
Methods 　Hepatoma SMMC-7721 cell strain was divided into blank control group and different concentrations of U0126 groups. The proliferation inhibition was measured by MTT assay. FCM was used to analyze the cell cycle distribution and apoptosis.
Results 　U0126 obviously inhibited cell proliferation, induced cell apoptosis and G0/G1 phase cell cycle arrest. There were significant differences between control group and different concentrations of U0126 groups on cell proliferation and apoptosis （P＜0.05， P＜0.01）.
Conclusion 　Blocking ERK1/2 pathway may be an important treatment strategy for liver cancer.

Citation： SHENG Wei,WANG Kang,PING Gaofeng,LI Xuqi,WEI Guangbing,HUANG Chen. Effects of Inhibit Extracellular-Signal Regulated Kinase 1/2 Pathway on Apoptosis of Hepatoma Carcinoma Cell SMMC-7721. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 675-678. doi: Copy

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1. 　Bos JL. All in the family? New insights and questions regarding interconnectivity of Ras, Rap1 and Ral [J]. EMBO J， 1998; 17(23)∶6776.
2. 　Steinmetz R, Wagoner HA, Zeng P, et al. Mechanisms regulating the constitutive activation of the ERK signaling pathway in ovarian cancer and the effect of RNAi for ERK1/2 on cancer cell proliferation [J]. Mol Endocrino, 2004; 15(3)∶1004.
3. 　Wu TT, Wang JS, Jiaan BP, et al. Role of p21(WAF1) and p27(KIP1) in predicting biochemical recurrence for organ-confined prostate adenocarcinoma [J]. J Chin Med Assoc， 2007; 70(1)∶11.
4. 　Handra-Luca A, Bilal H, Bertrand JC, et al. Extra-cellular signal-regulated ERK-1/ERK-2 pathway activation in human salivary gland mucoepidermoid carcinoma: association to aggressive tumor behavior and tumor cell proliferation [J]. Am J Pathol， 2003; 163(3)∶957.
5. 　Hoshino R, Chatani Y, Yamori T, et al. Constitutive activation of the 41-/43-kDa mitogen-activated protein kinase signaling pathway in human tumors [J]. Oncogene， 1999; 18(3)∶813.
6. 　Mishima K, Inoue K, Hayashi Y. Overexpression of extracellular-signal regulated kinases on oral squamous cell carcinoma [J]. Oral Oncol， 2002; 38(5)∶468.
7. 　郑铭，张幼怡，韩启德. β肾上腺素受体的丝裂原活化蛋白激酶信号途径 [J]. 生理科学进展, 2003; 33(2)∶111.
8. 　Kolch W. Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions [J]. Biochem J， 2000; 351（Pt 2）∶289.
9. 　Gysin S, Lee SH, Dean NM, et al. Pharmacologic inhibition of RAF→MEK→ERK signaling elicits pancreatic cancer cell cycle arrest through induced expression of p27Kip1 [J]. Cancer Res， 2005; 65(11)∶4870.
10. 　Ito Y, Sasaki Y, Horimoto M, et al. Activation of mitogen-activated protein kinases/extracellular signal-regulated kinases in human hepatocellular carcinoma [J]. Hepatology， 1998; 27(4)∶951.
11. 　Adeyinka A, Nui Y, Cherlet T, et al. Activated mitogen-activated protein kinase expression during human breast tumorigenesis and breast cancer progression [J]. Clin Cancer Res， 2002; 8(6)∶1747.
12. 　Albanell J, Codony-Servat J, Rojo F, et al. Activated extracellular signal-regulated kinases: association with epidermal growth factor receptor/transforming growth factor alpha expression in head and neck squamous carcinoma and inhibition by anti-epidermal growth factor receptor treatments [J]. Cancer Res， 2001; 61(17)∶6500.
13. 　Uzgare AR, Kaplan PJ, Greenberg NM. Differential expression and/or activation of P38MAPK, erk1/2, and jnk during the initiation and progression of prostate cancer [J]. Prostate， 2003; 55(2)∶128.
14. 　Rincon-Arano H, Rosales R, Mora N, et al. R-Ras promotes tumor growth of cervical epithelial cells [J]. Cancer， 2003; 97(3)∶575.
15. 　Gioeli D, Mandell JW, Petroni GR, et al. Activation of mitogen-activated protein kinase associated with prostate cancer progression [J]. Cancer Res， 1999; 59(2)∶279.
16. 　Anders M, Christian C, McMahon M, et al. Inhibition of the Raf/MEK/ERK pathway up-regulates expression of the coxsackievirus and adenovirus receptor in cancer cells [J]. Cancer Res， 2003; 63(9)∶2088.
17. 　Wen J, Zhang Y, Chen X, et al. Enhancement of diallyl disulfide-induced apoptosis by inhibitors of MAPKs in human HepG2 hepatoma cells [J]. Biochem Pharmacol， 2004; 68(2)∶323.
18. 　黄辰，刘利英，宋土生，等. siRNA介导的MAPK p42沉默诱导HeLa细胞凋亡 [J]. 南方医科大学学报， 2006; 26(1)∶11.
19. 　Tamemoto H, Kadowaki T, Tobe K, et al. Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells [J]. J Biol Chem， 1992; 267(28)∶20293.
20. 　黄辰，刘利英，宋土生，等. 使用技术抑制细胞P42MAPK表达 [J]. 中华病理学杂志, 2006； 35(5)∶292.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Inhibit Extracellular-Signal Regulated Kinase 1/2 Pathway on Apoptosis of Hepatoma Carcinoma Cell SMMC-7721

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