Effects of Survivin Antisense RNA on Apoptosis and Reversing Drug Resistance of SGC7901 Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Xihai , ZHANG Qifan , MA Rong

The Fourth Affiliated Hospital， Harbin Medical University, Harbin 150001, China;

Corresponding author：

Keywords：

Survivin; Antisense; Apoptosis; Taxotere; Multi-drug resistance gene

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Objective 　To study the effects of survivin antisense RNA on SGC7901 cell’s apoptosis and chemosensitivity to taxotere, and to investigate its effect on the expression of multi-drug resistance gene-1 (MDR-1).
Methods 　Survivin antisense eukaryotic vector anti-pcDNA3-svv was transfected into SGC7901 cell lines by lipofectamine and positive clones were screened out then. Survivin protein and MDR-1 mRNA were measured by western blot and RT-PCR, respectively. Apoptosis that was induced by anti-pcDNA3-svv was observed by electronic microscope, and the sensitivity of SGC7901 cell to taxotere was examined by MTT.
Results 　The expressions of survivin protein and MDR-1 mRNA in transfected SGC7901 cells both decreased more significantly than that of non-transfected cells (P＜0.05， P＜0.01), and the indices of MDR of transfection group and non-transfection group were 0.196±0.013 and 3.126±0.019, respectively, at the late phase of apoptosis, which had a significant difference between each other (P＜0.01), IC50 of the transfected cells to taxotere was （16.7±1.98） ng/ml and that of the non-transfected cells was (55.7±1.89) ng/ml, which also had a significant difference (P＜0.01).
Conclusion 　Surivivin antisense RNA could induce the apoptosis of SGC7901 cancer cell line and could increase the cells’ sensitivity to taxotere, which may help to reverse drug resistance.

Citation： CHEN Xihai,ZHANG Qifan,MA Rong. Effects of Survivin Antisense RNA on Apoptosis and Reversing Drug Resistance of SGC7901 Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 63-67. doi: Copy

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11.	韩艳秋，袁家颖，石永进，等. 小檗胺逆转K562/A02细胞耐药性及其机制 [J]. 中国实验血液学杂志， 2003; 11(6)∶604.

1. 　姚学权，刘福坤，祁晓萍，等. 胃腺癌组织survivin基因的表达与细胞增殖及凋亡的相关性研究 [J]. 中华外科杂志， 2004； 42（3）∶145.
2. 　Trieb K, Lehner R, Stulnig T, et al. Survivin expression in human osteosarcoma is a marker for survival [J]. Eur J Surg Oncol， 2003; 29(4)∶379.
3. 　Chen LT, Liu TW, Wu CW, et al. A phase Ⅰ study of weekly docetaxel, 24-hour infusion of high-dose fluorouracil/leucovorin and cisplatin in patients with advanced gastric cancer [J]. Oncology， 2002; 63(3)∶239.
4. 　Suzuki T, Yoshida K, Tanabe K, et al. Three advanced gastric cancer patients successfully treated by combination therapy of docetaxel and TS-1 [J]. Gan To Kagaku Ryoho， 2005; 32(4)∶509.
5. 　Shin S, Sung BJ, Cho YS, et al. An anti-apoptotic protein human survivin is a direct inhibitor of caspase-3 and-7 [J]. Biochemistry， 2001; 40(4)∶1117.
6. 　Giodini A, Kallio MJ, Wall NR, et al. Regulation of microtubule stability and mitotic progression by survivin [J]. Cancer Res， 2002; 62(9)∶2462.
7. 　Lens SM, Wolthuis RM, Klompmaker R, et al. Survivin is required for a sustained spindle checkpoint arrest in response to lack of tension [J]. EMBO J， 2003; 22(12)∶2934.
8. 　Zaffaroni N, Daidone MG. Survivin expression and resistance to anticancer treatments: perspectives for new therapeutic interventions [J]. Drug Resist Updat， 2002; 5(2)∶65.
9. 　Bali P, Pranpat M, Swaby R, et al. Activity of suberoylanilide hydroxamic acid against human breast cancer cells with amplification of her-2 [J]. Clin Cancer Res， 2005; 11(17)∶6382.
10. 　Notarbartolo M, Cervello M, Dusonchet L, et al. Resistance to diverse apoptotic triggers in multidrug resistant HL60 cells and its possible relationship to the expression of P-glycoprotein, Fas and of the novel anti-apoptosis factors IAP (inhibitory of apoptosis proteins) [J]. Cancer Lett， 2002; 180(1)∶91.
11. 　韩艳秋，袁家颖，石永进，等. 小檗胺逆转K562/A02细胞耐药性及其机制 [J]. 中国实验血液学杂志， 2003; 11(6)∶604.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Survivin Antisense RNA on Apoptosis and Reversing Drug Resistance of SGC7901 Cells

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