Objective To study the significance of the levels of plasma inflammatory cytokines (IL-6，IL-8，IL-10 and TNF-α) in patients with acute deep venous thrombosis (DVT) of lower extremity. Methods Forty untreated DVT cases were selected as the subjects in the DVT group, while thirty healthy subjects, whose ages and genders showed no significant difference with the DVT patients, were collected as the control group. The plasma levels of IL-6, IL-8 and TNF-α were detected by radioimmunoassay (RIA), and the plasma level of IL-10 was measured by enzyme-linked immunosorbent assay (ELISA). Correlation analysis was used to investigate the relationships between the levels of different inflammatory cytokines within DVT group. Results The levels of plasma cytokines in the DVT group were all significantly higher than those in control group (P＜0.001). The results of the correlation analysis showed that there were positive correlations between IL-6 and TNF-α (r=0.383, P＜0.05), IL-10 and TNF-α (r=0.390, P＜0.05), respectively, within the DVT group; whereas there were no correlations between IL-6 and IL-8, IL-6 and IL-10, IL-8 and IL-10, and IL-8 and TNF-α. Conclusion The levels of plasma cytokines increased significantly in patients of DVT. Inflammatory cytokines may play an important role in acute DVT by accelerating the pace of thrombosis, intensifying the inflammatory reaction around thrombus and aggravating the injured blood vessel.
Citation：
ZHOU Xiangyu,HE Yanzheng,HE Chunshui,LIU Yong.. Level and Significance of Inflammatory Cytokines in Patients with Acute Deep Venous Thrombosis of Lower Extremity. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(6): 653-655. doi:
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- 1. Reitsma PH, Rosendaal FR. Activation of innate immunity in patients with venous thrombosis: the Leiden Thrombophilia Study [Ｊ]. J Thromb Haemost， 2004; 2(4)∶619.
- 2. Fichtlscherer S, Breuer S, Heeschen C, et al. Interleukin-10 serum levels and systemic endothelial vasoreactivity in patients with coronary artery disease [Ｊ]. J Am Coll Cardiol， 2004; 44(1)∶44.
- 3. Roumen-Klappe EM, den Heijer M, van Uum SH, et al. Inflammatory response in the acute phase of deep vein thrombosis [Ｊ]. J Vasc Surg， 2002; 35(4)∶701.
- 4. Suwa T, Hogg JC, Quinlan KB, et al. The effect of interleukin-6 on L-selectin levels on polymorphonuclear leukolytes [Ｊ]. Am J Physiol Heart Circ Physiol, 2002; 283(3)∶H879.
- 5. Min W, Bradley JR, Galbraith JJ, et al. The N-terminal domains target TNF receptor-associated factor-2 to the nucleus and display transcriptional regulatory activity [Ｊ]. J Immunol， 1998; 161(1)∶319.
- 6. Eppihimer MJ, Schaub RG. P-Selectin-dependent inhibition of thrombosis during venous stasis [Ｊ].Arterioscler Thromb Vasc Biol， 2000; 20(11)∶2483.
- 7. Henke PK, Wakefield TW, Kadell AM, et al. Interleukin-8 administration enhances venous thrombosis resolution in a rat model [Ｊ]. J Surg Res， 2001; 99(1)∶84.
- 8. Myers DD Jr, Hawley AE, Farris DM, et al. Cellular IL-10 is more effective than viral IL-10 in decreasing venous thrombosis [Ｊ]. J Surg Res， 2003; 112(2)∶168.
- 9. Cassatella MA, Gasperini S, Bovolenta C, et al. Interleukin-10 (IL-10) selectively enhances CIS3/SOCS3 mRNA expression in human neutrophils: evidence for an IL-10-induced pathway that is independent of STAT protein activation [Ｊ]. Blood， 1999; 94(8)∶2880.
- 10. van Haelst PL, Tervaert JW, Bijzet J, et al. Circulating monocytes in patients with acute coronary syndromes lack sufficient interleukin-10 production after lipopolysaccharide stimulation [Ｊ]. Clin Exp Immunol， 2004; 138(2)∶364.
