Establishment of Adriamycin-Resistant Human Pancreatic Cancer SW1990/ADM Cell Line and to Investigate Its Resistance Mechanism_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Liyang , ZHAO Yupei , WU Yuande , LIAO Quan , GUO Junchao , LIU Ziwen , ZHANG Taiping.

Department of General Surgery, PUMC Hospital, Peking Union Medical College (PUMC), Chinese Academy of Medical Sciences， Beijing 100730, China;

Corresponding author：

Keywords：

Pancreatic cancer Adriamycin Drug resistance

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【Abstract】ObjectiveTo establish adriamycin (ADM) resistant pancreatic cancer cell line SW1990/ADM and to investigate its drug resistance mechanism.
MethodsADM-resistant pancreatic cancer cell line SW1990/ADM was obtained by culture of pancreatic cancer cell line SW1990 in vitro with intermittently increasing the concentration of ADM in the culture medium for ten months. After two months of drug free culture, its biological characteristics, drug sensitivity as well as the expression and function of multidrug resistant gene 1 (mdr1) were detected, respectively.
ResultsCompared with the parental cell line, SW1990/ADM showed great changes in biological characteristics and developed a cross resistance to various chemotherapy drugs. The drug resistance indexes of cell line SW1990/ADM to ADM, mitomycin, fluorouracil and gemcitabine were 49.60, 7.25, 3.80 and 1.25, respectively. The level of mdr1 mRNA expression in cell line SW1990/ADM was much higher than that of the parental cell line（P＜0.01).
ConclusionWe have established adriamycin resistant pancreatic cancer cell line SW1990/ADM with multidrug resistance phenotype, its multidrug resistance is positively relevant to the expression of mdr1.

Citation： ZHANG Liyang,ZHAO Yupei,WU Yuande,LIAO Quan,GUO Junchao,LIU Ziwen,ZHANG Taiping.. Establishment of Adriamycin-Resistant Human Pancreatic Cancer SW1990/ADM Cell Line and to Investigate Its Resistance Mechanism. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(1): 46-50. doi: Copy

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1. Fillpits M, Suchomel RW, Dekan G, et al. Expression of the multidrug resistanceassociated protein (MRP) gene in colorectal carcinomas [Ｊ]. Br J Cancer, 1997; 75(2)∶208.
2. 张立阳,赵玉沛,廖泉,等. MDR1在胰腺癌细胞株中的表达及其意义 [Ｊ]. 中华实验外科杂志， 2003； 20（5）∶475.
3. Jokoby WB. Methods in enzymology [M]. New York: Acad Press,1979∶150.
4. Hansen MB, Nielsen SE, Berg K. Reexamination and further development of a precise and rapid dye method for measuring cell growth/cell kill [Ｊ]. J Immunol Methods, 1989; 119(2)∶203.
5. Biedler JL, Riehm H. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: crossresistance, radioautographic, and cytogenetic studies [Ｊ]. Cancer Res, 1970; 30(4)∶1174.
6. Suwa H, Ohshio G, Arao S, et al. Immunohistochemical localization of Pglycoprotein and expression of the multidrug resistance1 gene in human pancreatic cancer: relevance to indicator of better prognosis [Ｊ]. Jpn J Cancer Res, 1996; 87(6)∶641.
7. 易善红,杨唐俊, 何斌,等. 体内基因转移建立多药耐药性人膀胱癌裸鼠荷瘤模型 [Ｊ]. 中华泌尿外科杂志, 2000; 21(9)∶547.
8. Yu DS, Chang SY, Ma CP. Characterization and modulation of transitional cell carcinoma cell lines with acquired multidrug resistance [Ｊ]. Br J Urol, 1998; 81(2)∶234.
9. 梁正东，卞度宏. 卵巢癌细胞对顺铂耐药及其逆转的实验研究 [Ｊ]. 中华妇产科杂志，1996； 31(2)∶75.
10. Moran E, Cleary I, Larkin AM, et al. Coexpression of MDRassociated markers, including P170, MRP and LRP and cytoskeletal proteins, in three resistant variants of the human ovarian carcinoma cell line, OAW42 [Ｊ]. Eur J Cancer, 1997; 33(4)∶652.
11. UchiyamaKokubu N, Watanabe T. Establishment and characterization of adriamycinresistant human colorectal adenocarcinoma HCT15 cell lines with multidrug resistance [Ｊ]. Anticancer Drugs, 2001; 12(9)∶769.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Adriamycin-Resistant Human Pancreatic Cancer SW1990/ADM Cell Line and to Investigate Its Resistance Mechanism

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