Expression of Extracellular SignalRegulated Kinase and p38 MitogenActivated Protein Kinase in Autogenous Vein Grafts_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Jun , HU Xinhua , ZHANG Qiang , DUAN Zhiquan.

Department of Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, China;

Corresponding author：

Keywords：

Vein graftMitogenactivated protein kinaseVascular remodeling

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ObjectiveTo investigate the expression of extracellular signalregulated kinase (ERK) and p38 mitogenactivated protein kinase （p38 MAPK） in autogenous vein grafts during vascular remodeling.MethodsAn autogenous vein graft model was established by transplanting the right jugular vein to infrarenal abdominal aorta in 80 Wistar rats. Vein graft samples were harvested 6 hours, 24 hours, 3 days, 7 days, 2 weeks, 4 weeks, 6 weeks and 8 weeks after surgery. Gene expression of ERK and p38 MAPK was measured by reverse transcriptionPCR. Western blot was used to detect the expression of protein products and phosphorylation protein products of ERK and p38 MAPK. Apoptosis of vascular smooth muscle cells (VSMCs) was determined by TUNEL. Proliferating cell nuclear antigen(PCNA) of VSMCs also was studied.ResultsThe expression of ERK1 mRNA and p38 MAPK mRNA increased considerably after surgery. ERK1 mRNA reached the peak on the 7th day 〔（33.2±14.2）%, P＜0.01〕, but p38 MAPK mRNA reached the peak on the second week after surgery 〔(58.8±26.2）%, P＜0.01〕. The expression of ERK1/2 detected by western blot reached the peak during 1 to 2 weeks and decreased gradually to normal level 6 weeks after surgery. The expression of p38 MAPK reached the peak during 2 to 4 weeks and decreased to 1/4 to 1/2fold 8 weeks after surgery. There was a positive relationship between ERK1 and PCNA(r=0.759 6，P＜0.01) and a positive relationship between p38 MAPK and apoptosis(r=0.892 2，P＜0.01). ConclusionActivation of MAPK system exists in autogenous vein grafts and it may become a new target for the therapy of stenosis after vein grafts.

Citation： YANG Jun,HU Xinhua,ZHANG Qiang,DUAN Zhiquan.. Expression of Extracellular SignalRegulated Kinase and p38 MitogenActivated Protein Kinase in Autogenous Vein Grafts. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 291-294. doi: Copy

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1. 冯勇，段志泉，胡海地，等. 移植静脉平滑肌细胞凋亡及相关基因表达与增殖关系的动态研究 [Ｊ]. 中华医学杂志， 1999; 79(1)∶ 15.
2. 胡新华，张强，孙达欣，等. 核转录因子κB及其抑制基因在自体移植静脉中的表达 [Ｊ]. 中华医学杂志， 2002; 82(22)∶1546.
3. Sambrook J, Fritsch EF, Maniatis T. 克隆化基因所表达蛋白质的检测与分析 [A]. 金冬雁，黎孟枫，译. 分子克隆实验指南 [M]. 第2版. 北京：科学出版社， 1992∶852～898.
4. Talmor D, Applebaum A, Rudich A, et al. Activation of mitogenactivated protein kinases in human heart during cardiopulmonary bypass [Ｊ]. Circ Res, 2000; 86(9)∶1004.
5. Thury A, van Langenhove G, Carlier SG, et al. High shear stress after successful balloon angioplasty is associated with restenosis and target lesion revascularization [Ｊ]. Am Heart J, 2002; 144(1)∶136.
6. Xi XP, Graf K, Goetze S,et al. Central role of the MAPK pathway in ang IImediated DNA synthesis and migration in rat vascular smooth muscle cells [Ｊ]. Arterioscler Thromb Vasc Biol, 1999; 19(1)∶73.
7. Xu Q, Liu Y, Gorospe M, et al. Acute hypertension activates mitogenactivated protein kinases in arterial wall [Ｊ]. J Clin Invest, 1996; 97(2)∶508.
8. Ohashi N, Matsumori A, Furukawa Y, et al. Role of p38 mitogenactivated protein kinase in neointimal hyperplasia after vascular injury [Ｊ]. Arterioscler Thromb Vasc Biol, 2000; 20(12)∶2521.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Extracellular SignalRegulated Kinase and p38 MitogenActivated Protein Kinase in Autogenous Vein Grafts

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