Effect of Recombinant Human Growth Hormone on Growth of Human Colonic Cancer Line COLO-320 Cells in Vitro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WEI Shoujiang ¹ , WANG Chongshu ¹ , WANG Yanqing ² , ZHAO Guogang ¹

Department of General Surgery, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China;

Corresponding author：

Keywords：

Recombinant human growth hormone; Colonic cancer; Inhibitory rate

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Objective To evaluate the effect of recombinant human growth hormone(rhGH) on growth of human colonic cancer cells (COLO-320) in vitro. Methods Human COLO-320 cells in logarithm growing period were cultured for 24 h,48 h or 72 h with variant concentrations of rhGH,camptothecine (CPT) or rhGH combined CPT in calf serum(serum group) or calf serum-free (serum-free group). Light density of cells were determined by MTT method, so that cellular inhibition rate were calculated.Results No influence on cell growth or inhibition rate was observed from cultures with variant concentrations and different acting times of rhGH (P＞0.05). Inhibition rate of single CPT or CPT combined rhGH were much more increased than single rhGH used (P＜0.01) with no statistical significance (P＞0.05).Conclusion The results show that rhGH has neither direct COLO-320 cells stimulation nor any evidence of COLO-320 cells inhibition, and has no influence of CPT on COLO-320 cells inhibition in vitro.

Citation： WEI Shoujiang,WANG Chongshu,WANG Yanqing,ZHAO Guogang. Effect of Recombinant Human Growth Hormone on Growth of Human Colonic Cancer Line COLO-320 Cells in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(6): 572-575. doi: Copy

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9.	OgilvyStuart AL, Shalet SM. Tumor occurrence and recurrence [Ｊ]. Horm Res, 1992； 38(Supple 1)∶50.
10.	许勤，马利民，桑剑峰，等. 生长激素对小鼠结肠腺癌肝转移瘤的影响 [Ｊ]. 肠外与肠内营养， 2002； 7(6)∶246.
11.	祁晓平，黎介寿，陈彻. 人生长激素对结肠癌细胞株细胞周期动力学的影响 [Ｊ]. 肠外与肠内营养， 2000； 7(2)∶83.
12.	Fiebig HH, Dengler W, Hendriks HR. No evidence of tumor growth stimulation in human tumors in vitro following treatment with recombinant human growth hormone [Ｊ]. Anti Cancer Drugs, 2000； 11(8)∶659.

1. Byrne TA, Morrissey TB, Gatzen C, et al. Anabolic therapy with growth hormone accelerates protein gain in surgical patients requiring nutritional rehabilitation [Ｊ]. Ann Surg, 1993； 218(4)∶400.
2. Balbis A, Bartke A, Turyn D. Overexpression of boving growth hormone in transgenic mice is associated with changes in hepatic insulin receptors and in their kinase activity [Ｊ]. Life Sci, 1996； 59(16)∶1363.
3. Carli F, Webster JD, Halliday D. A nitrogenfree hypocaloric diet and recombinant human growth hormone stimulate postoperative protein synthesis: fasted and fed leucine kinetics in the surgical patient [Ｊ]. Metabolism, 1997； 46(7)∶796.
4. Berman RS, Harrison LE, Pearlstone DB, et al. Growth hormone alone and in combination with insulin, increases whole body and skeletal muscle protein kinetics in cancer patients after surgery [Ｊ]. Ann Surg, 1999； 229(1)∶1.
5. 李立，冉江华，张炳彦，等. 应用重组人生长激素对肝胆胰外科患者术后氮平衡及营养状况的影响 [Ｊ]. 中国普外基础与临床杂志， 2000； 7(6)∶383.
6. 冉江华，郭群，张家骅，等. 胃肠道恶性肿瘤患者应用重组人生长激素后免疫功能的变化 [Ｊ]. 中国普外基础与临床杂志， 1999； 6(5)∶298.
7. 陈嘉勇，张捷，谭晶，等. 胃、结肠癌患者术后生长激素应用的价值评估 [Ｊ]. 中国普外基础与临床杂志， 1999； 6(6)∶365.
8. Harrison LE, Blumberg D, Berman R, et al. Effect of human growth hormone on human pancreatic carcinoma growth, protein, and cell cycle kinetics [Ｊ]. J Surg Res, 1996； 61(2)∶317.
9. OgilvyStuart AL, Shalet SM. Tumor occurrence and recurrence [Ｊ]. Horm Res, 1992； 38(Supple 1)∶50.
10. 许勤，马利民，桑剑峰，等. 生长激素对小鼠结肠腺癌肝转移瘤的影响 [Ｊ]. 肠外与肠内营养， 2002； 7(6)∶246.
11. 祁晓平，黎介寿，陈彻. 人生长激素对结肠癌细胞株细胞周期动力学的影响 [Ｊ]. 肠外与肠内营养， 2000； 7(2)∶83.
12. Fiebig HH, Dengler W, Hendriks HR. No evidence of tumor growth stimulation in human tumors in vitro following treatment with recombinant human growth hormone [Ｊ]. Anti Cancer Drugs, 2000； 11(8)∶659.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Recombinant Human Growth Hormone on Growth of Human Colonic Cancer Line COLO-320 Cells in Vitro

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