The Effects XGD1 on the Proliferation of Human Peripheral Blood T Lymphocyte and the Expression of IL2R_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Li , ZHUO Shourong

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical College, Kunming 650101， China;

Corresponding author：

Keywords：

XGD1; Lymphocytic proliferation; IL2 receptor

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo explore the immunosuppressive effect of XGD1 and its mechanism. MethodsDifferent concentrations of XGD1 were added to PHA or ConA induced human peripheral blood T lymphocyte.Seventytwo hours later modified MTT assay was employed to test the effect of XGD1 on T cell proliferation. Flowcytometry was used to examine the effect of XGD1 on the expression of IL2 receptor(IL2R) on the surface of T cells individually at 48 h and 72 h.And the effects of XGD1 combined with cyclosporine A(CsA) on the proliferation of Tlymphocytes and the expression of IL2R were also investigated. ResultsIn the concentration range of 0.2～25 μg/ml,XGD1 exerted marked inhibitory effect on PHA or ConA induced T cell proliferation,which was proportional to dose. Flowcytometry showed that XGD1 inhibited the expression of IL2R,and the percentage of IL2Rα positive cells after stimulation of PHA decreased from 47.67% to 25.03% in the presence of XGD1 (1 μg/ml).XGD1 and CsA had synergism in inhibition of T cell proliferation and IL2R expression. ConclusionThe study suggests that XGD1 has immunosuppressive effect. The suppressive effect of XGD1 on T cell proliferation is most probably mediated by decreasing IL2R expression.

Citation： LI Li,ZHUO Shourong. The Effects XGD1 on the Proliferation of Human Peripheral Blood T Lymphocyte and the Expression of IL2R. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 230-233. doi: Copy

1.	余传霖,叶天星,陆德源,等主编.现代医学免疫学 [M]. 上海: 上海医科大学出版社,1998∶1257～1267.
2.	孙秀珍，谢蜀生，龙振洲，等. 青嵩素及其衍生物免疫抑制作用的实验研究 [J]. 中西医结合杂志，1991； 11（1）∶37.
3.	张雷,朱彤,闵志廉，等.FTY 720抑制ConA 诱导小鼠T 细胞CD25 的表达 [J].上海免疫学杂志, 2002; 22(5)∶344.
4.	GonzalezGarcia AC, Merida I, Martinez A, et al.Intermediate affinity interleukin 2 receptor mediates survival via a phosphatidylinositol 3kinasedependent pathway [J]. J Biol Chem, 1997; 272(15)∶10220.
5.	朱迅,杨贵贞.人外周血淋巴细胞IL2Rα 表达特性及动力学 [J].免疫学杂志,1990; 6(1)∶22.
6.	苏泽轩, 于立新, 黄洁夫主编. 现代移植学 [M]. 北京: 人民卫生出版社, 1998∶48～50.
7.	Calne R. Immunosuppression for organ grafting [J]. Transplant Proc, 1992; 24(4)∶1260.
8.	Hess AD,Petcher TJ,Ruegger A.Effect of interleukin 2 on the immunosuppressive action of cyclosporine [J].Transplantation,1995; 39(1)∶62.
9.	姜汉英，卢晓辉，廖永芳.国产免疫抑制剂F904 对T 细胞激活的抑制及IL2的调节作用 [J].中华器官移植杂志,1997; 18(2)∶77.

1. 余传霖,叶天星,陆德源,等主编.现代医学免疫学 [M]. 上海: 上海医科大学出版社,1998∶1257～1267.
2. 孙秀珍，谢蜀生，龙振洲，等. 青嵩素及其衍生物免疫抑制作用的实验研究 [J]. 中西医结合杂志，1991； 11（1）∶37.
3. 张雷,朱彤,闵志廉，等.FTY 720抑制ConA 诱导小鼠T 细胞CD25 的表达 [J].上海免疫学杂志, 2002; 22(5)∶344.
4. GonzalezGarcia AC, Merida I, Martinez A, et al.Intermediate affinity interleukin 2 receptor mediates survival via a phosphatidylinositol 3kinasedependent pathway [J]. J Biol Chem, 1997; 272(15)∶10220.
5. 朱迅,杨贵贞.人外周血淋巴细胞IL2Rα 表达特性及动力学 [J].免疫学杂志,1990; 6(1)∶22.
6. 苏泽轩, 于立新, 黄洁夫主编. 现代移植学 [M]. 北京: 人民卫生出版社, 1998∶48～50.
7. Calne R. Immunosuppression for organ grafting [J]. Transplant Proc, 1992; 24(4)∶1260.
8. Hess AD,Petcher TJ,Ruegger A.Effect of interleukin 2 on the immunosuppressive action of cyclosporine [J].Transplantation,1995; 39(1)∶62.
9. 姜汉英，卢晓辉，廖永芳.国产免疫抑制剂F904 对T 细胞激活的抑制及IL2的调节作用 [J].中华器官移植杂志,1997; 18(2)∶77.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Effects XGD1 on the Proliferation of Human Peripheral Blood T Lymphocyte and the Expression of IL2R

Abstract Full text Figures/Tables Video References Cited by

Format

Content