Study on p27KIP1 Expression in Hepatocellular Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHENG Jianyong ¹ , LI Kaizong ² , WANG Weizhong ¹ , LI Jiang ³

Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China;

Corresponding author：

Keywords：

Hepatocellular carcinoma; Gene; Gene expression

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ObjectiveTo study the relationship between expression of p27KIP1 and progression of hepatocellular carcinoma(HCC).MethodsThe expression of p27KIP1 in 52 cases of HCC was detected by immunohistochemistry of strept avidinbiotin complex and mRNA in situ hybridization.ResultsThe positive cells of p27KIP1 protein were diffused in HCC.The positive signal was localized in nuclei.The labeling index (LI) of p27KIP1 protein was significantly higher in tumorsurrounding tissues than that in tumor tissues. p27KIP1 protein LI showed a positive correlation with the differentiation grade of HCC.The better differentiation of cancer cells, the higher LI of p27KIP1 protein (P＜0.01).The positive cells of p27KIP1 mRNA were also diffused in HCC.The positive signal was localized in nuclei and cytoplasm. As to the expression of p27KIP1 at the mRNA level,there was no significant correlation with tumorsurrounding tissues and stages of HCC.Conclusionp27KIP1 protein is associated with progression and differentiation grade of hepatocellular carcinoma.

Citation： ZHENG Jianyong,LI Kaizong,WANG Weizhong,LI Jiang. Study on p27KIP1 Expression in Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 240-242. doi: Copy

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1. Lloyd RV, Erickson LA, Jin L, et al. p27KIP1, a multifunctional cyclindependent kinase inhibitor with prognostic significance in human cancers [J]. Am J Pathol, 1999; 154(2)∶ 313.
2. Peng D, Fan Z, Lu Y, et al. Antiepidexmal growth factor receptor monoclonal antibody 225 upregulates p27KIP1and induces G1 arrest in prostatic cancer cell line DU1451 [J]. Cancer Res, 1996; 56（16）∶ 3666.
3. Polyak K, Kato JY, Solomon MJ, et al. p27KIP1, a cyclinCDK inhibitor; links transforming growth factorbeta and contact inhibition to cell cycle arrest [J]. Genes Dey, 1994; 8（1）∶ 9.
4. 郭贵龙，姚榛祥. p27在甲状腺滤泡状肿瘤中的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（5）∶ 324.
5. 夏加增，杨继昌，张熔熔,等. 胃癌中cyclin D1、cyclin E和p27的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（3）∶ 148.
6. Tsihlias J, Kapusta LR, DeBoer G, et al. Loss of cyclindependent kinase inhibitor p27KIP1 is a novel prognostic factor in localized human prostate adenocarcinoma [J]. Cancer Res, 1998; 58（3）∶ 542.
7. Kawamata N, Morosetti R, Miller CW, et al. Molecular analysis of the cyclindependent kinase inhibitor gene p27/Kip1 in human malignancies [J]. Cancer Res, 1995; 55（11）∶ 2266.
8. Zhang Y, Lin SC. Molecular characterization of the cyclindependent kinase inhibitor p27 promoter [J]. Biochim Biophys Acta, 1997; 1353(3)∶ 307.
9. Loda M, Cukor B, Tam SW, et al. Increased proteasomedependent degradation of the cyclindependent kinase inhibitor p27 in aggressive colorectal carcinomas [J]. Nat Med, 1997; 3（2）∶ 231.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on p27KIP1 Expression in Hepatocellular Carcinoma

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