ObjectiveTo investigate the expression of heat shock proteins 90α(HSP90α) in human hepatocellular carcinoma and the relationship between its expression and biologic behavior of tumor and prognosis. MethodsUsing the immunohistochemical SP method, HSP90α expression was detected in liver tissue from 10 normal individuals, 40 patients with hepatocellular carcinoma(HCC) and adjacent noncancerous liver tissues. ResultsThe positive expression rate of HSP90α was 10.0%,52.5%,72.5% in normal liver tissues,adjacent noncancerous liver tissues,hepatocellular carcinous tissues respectively. A significantly higher distribution of HSP90α positive expression in HCC tissues compared with adjacent noncancerous liver tissues and normal liver tissues was obtained （P＜0.05）. The positive expression of HSP90α in HCC was correlated with clinical stage, tumor differentiation, serosal condition and lymph node metastasis （P＜0.05）, but not to tumor number （P＞0.05）. It was also correlated with prognosis of HCC. The mean tumorfree survival of patients with HSP90α negative expression was 38.6 months while that of HSP90α positive expression was 25.5 months （P＜0.05）. ConclusionHSP90α is overexpressed in human hepatocellular carcinoma. HSP90α could be used as an indicator to judge the clinical stage, tumor differentiation, serosal condition, lymph node metastasis and prognosis of HCC.
Citation：
SUN Shuiping,WU Shengli,GENG Zhimin,NIU Xinjie,YANG Wei. Expression of Heat Shock Protein 90α in Hepatocellular Carcinoma and Its Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 243-245. doi:
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- 1. Mizushima Y, Wang P, Jarrar D, et al. Preinduction of heat shock proteins protects cardiac and hepatic functions following trauma and hemorrhage [J]. Am J Physiol Regul Integr Comp Physiol，2000; 278(2)∶R352.
- 2. 华赟鹏，梁力建，黄洁夫. 热休克蛋白与肝缺血再灌注损伤 [J]. 中国普外基础与临床杂志， 2000; 7(4)∶269.
- 3. Hartl FU. Molecular chaperones in cellular protein folding [J]. Nature, 1996; 381(6583)∶571.
- 4. Kapitanovic M. Mechanical ileus caused by strangulation of the small intestine in a left paraduodenal hernia and in a defect in the root of the mesentery [J]. Lijec Vjesn, 1989； 111(12)∶454.
- 5. Yano M, Naito Z, Tanaka S, et al. Expression and roles of heat shock proteins in human breast cancer [J]. Jpn J Cancer Res, 1996; 87(9)∶ 908.
- 6. Blagosklonny MV, Toretsky J, Bohen S, et al. Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90 [J]. Proc Natl Acad Sci USA, 1996; 93(16)∶8379.
- 7. 黄常志，周纯，赵玫，等. 反义热休克蛋白90 β对HeLa细胞恶性表型和药敏感性的影响 [J]. 中华肿瘤杂志， 2000; 22(1)∶14.
- 8. Jameel A, Skilton RA, Campbell TA, et al. Clinical and biological significance of HSP90α in human breast cancer [J]. Int J Cancer, 1992; 50(3)∶409.
- 9. Mileo AM, Fanuele M, Battaglia F, et al. Selective overexpression of mRNA coding for 90 kDa stress protein in human ovarian cancer [J]. Anticancer Res, 1990; 10(4)∶903.
- 10. Nanbu K, Konishi I, Komatsu T, et al. Expression of heat shock proteins HSP70 and HSP90 in endometrial carcinomas. Correlation with clinicopathology, sex steroid receptor status, and p53 protein expression [J]. Cancer, 1996; 77(2)∶330.
