ObjectiveTo investigate the methodology of a newly developed highpressure liquid chromatography electrochemical system in urinary 8hydroxydeoxyguanosine (8OHdG) quantification. MethodsQuantification of urinary 8OHdG with ESA 5600 Coularray system among 21 children from liver cancer highrisk areas, Fusui country, in contrast with 63 controls from Nanning city, Guangxi province.ResultsThe resolution, sensitivity, linearity, precision and recovery of this approach all fully meet the requirement of routine urinary 8OHdG quantification. The mean 8OHdG level of this population was (5.26±0.41) ng/mg creatinine, higher than previous report 〔(4.62±0.091) ng/mg creatinine〕 by similar method.ConclusionWith cautious design and modification, this method is reliable and accurate in high throughput quantification of urinary 8OHdG.
Citation: PENG Tao,YAN Lnan,LIU Zhiming,Hiroshi Kasai,PENG Minhao,LI Lequn,Kazuyoshi Tamae,QIN Xue,WEI Zhongliang,LIANG Renxiang,LI Jiaquan. Measurement of Urinary 8Hydroxydeoxyguanosine Level with a HighPressure Liquid Chromatography Eectrochemical Approach. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 281-283. doi: Copy
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