Objective To investigate the inhibitory effect of proliferation cell nuclear antigen (PCNA) antisense oligonucleotides mediated by liposome transfection on hepatocellular carcinoma cell proliferation. MethodsThe antisense oligonucleotides were complementary to 18mer sequences next to the start codon of PCNA mRNA sequences. The human hepatocellular carcinoma cell line Bel7404 was treated with antisense oligonucleotides. The inhibition of proliferation was estimated by MTT method. We compared the deference between the liposome mediated transfection technique and direct transfection technique. ResultsThe cell proliferation was inhibited effectively by antisense oligonucleotides. A sense sequence oligomer showed no effect.Liposome mediated transfection could enhance the inhibitory effect. Conclusion Liposome mediated transfection could enhance the inhibitory effect of PCNA antisense oligonucleotides on hepatocellular carcinoma cell proliferation.
Citation：
ZHU Jinhai,CHEN Yanling,HUANG Jianfu,et al. Inhibition of Hepatocellular Carcinoma Cell Proliferation by Antisense Oligonucleotides Targeting the Messenger RNA Encoding Proliferation Cell Nuclear Antigen Mediated by Liposome Transfection. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(2): 93-95. doi:
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- 1. Jaskulski D, Deriel JK, Mercer WE, et al. Inhibition of cellular proliferation by antisense oligonucleotides to pcna cyclin \[J\]. Science， 1988； 240（4858）∶1544.
- 2. Travali S, Ku DH, Rizzo MG, et al. Structure of human gene for the proliferating cell nuclear antigen \[J\]. J Biol Chem， 1989； 264（13）∶7466.
- 3. 鄂征.组织培养和分子细胞学技术 \[M\].第2版.北京： 北京出版社，1997∶155~156.
- 4. Sakakura C, Hagiwara A, Tsujimoto H, et al. Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen \[J\].Br J Cancer，1994； 70(6)∶1060.
- 5. Taniai M,Toimatsu M,Okuda H.Immunohistochemical detection of proliferating nuclear antigen in hepatocellular carcinoma relationship to histological grade \[J\]. J Gastroenterol Hepatol,1993； 8（5）∶420.
- 6. 史宪杰, 李开宗, 窦科峰，等.增殖细胞核抗原在肝硬化、肝细胞不典型增生和肝细胞癌中的表达及意义 \[J\].中华实验外科杂志,1998； 15(5)∶412.
