The Role of Apoptosis of Acinar Cells in Acute Rejection after Pancreas Transplantation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of Surgery, Cancer Hospital/Institute Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021;

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Keywords：

Pancreas transplantation Graft rejection Cell apoptosis Histopathology

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ObjectiveTo explore apoptosis of acinar cells during pancreatic allograft rejection in rats.MethodsGroups of Wistar rats underwent heterotopic pancreaticoduodenal transplantation from syngenic Wistar of allogenic SD rats. The grafts were harvested on postoperative day 3, 5 and 7. All graft samples were subjected to histological examination and apoptotic cells of graft acinar cells using in situ terminal deoxynucleotidy1 transferasemediated dUTP nickend labeling (TUNEL). Histopathological rejection score and apoptotic index (AI) were analyzed. ResultsThe incidence of apoptotic cells was increased steadily over time in allografts, in contrast with syngenic grafts. The apoptotic cells in allografts were mainly acinar cells and few infiltrating lymphocytes. A significant correlation between apoptotic index and histopathological rejection score was noted.ConclusionTUNEL can display apoptosis of single cell in situ. Apoptosis is an important mechanism of tissue injury in acute pancreatic allograft rejection in rats. Acinar cell apoptosis can be used as a valuble index to estimate the injury of grafts and to monitor the acute rejection.

Citation： NI Xiaoguang,GUAN Fenglin,LIU Zhong,et al.. The Role of Apoptosis of Acinar Cells in Acute Rejection after Pancreas Transplantation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(6): 392-394. doi: Copy

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1. Kabelitz D. Apoptosis, graft rejection, and transplantation tolerance [Ｊ]. Transplantation, 1998; 65(7)∶869.
2. Gavrieli Y, Sherman Y, BenSassion SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [Ｊ]. J Cell Biol, 1992; 119(3)∶493.
3. Lee S, Tung KS, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat [Ｊ]. Transplantation, 1972; 13(4)∶421.
4. 孙治君，姚榛祥. 输入供体血对延长大鼠移植胰腺功能存活的初步研究 [Ｊ]. 中国普外基础与临床杂志，2001； 8（6）∶364.
5. Nakhleh RE, Sutherland DE, Tzardis P, et al. Correlation of rejection of the duodenum with rejection of the pancreas in a pig model of pancreaticoduodenal transplantation [Ｊ]. Transplantation, 1993; 56(6)∶1353.
6. Shresta S, Pham CT, Thomas DA, et al. How do cytotoxic lymphocytes kill their targets? [Ｊ]. Curr Opin Immunol, 1998; 10(5)∶581.
7. Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as the major mechanisms of T cellmediated cytotoxicity [Ｊ]. Science, 1994; 265(5171)∶528.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Role of Apoptosis of Acinar Cells in Acute Rejection after Pancreas Transplantation in Rats

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