• .Institute of Respiratory Diseases of PLA,Xinqiao Hospital,The Third Military Medical University.Chongqing,400037,ChinaCorresponding author:LI Qi,E-mail:liqioliver@sina.com;
LI Qi, Email: liqioliver@sina.com
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Objective To investigate the effect of aerosolized perfluorocarbon (PFC) (FC77) on gas exchange,histopathological changes of lung in acute lung injury and pulmonary expression of tumor necrosis factor-α (TNF-α) mRNA.Methods After acute lung injury (ALI) was induced by oleic acid (OA),16 rabbits were assigned randomly into 2 groups,ie.aerosolized perfluorocarbon group (PFC group) and conventional mechanical ventilation group (CMV group).Gas exchange parameters were measured before and after ALI,at 1,2,3,4 h after treatment.Histological sections taken from 6 different parts of lung were stained by hematoxylin and eosion.The express of TNF-α mRNA in the 2 different parts of lung were detected by in situ hybridization (ISH).Results Compared with CMV group,the PaO2 and static lung compliance (CLst) were significantly increased (P lt;0.05),the histopathological lesions of lung were attenuated,and the TNF-α mRNA expression was decreased significantly in PFC group (all P lt;0.05).There was more expression of TNF-α mRNA in backside than that in foreside of lung in two groups (P lt;0.05).Conclusion Aerosolized perfluorocarbon (PFC) can decrease expression of tumor necrosis factor-α mRNA in the lung,and improve the CLst and oxygenation during acute lung injury.

Citation: HUANG Zhijian,LI Qi,WANG Changzheng,QIAN Guisheng,SONG Peng. Effect of aerosolized perfluorocarbon on the tumor necrosis factor-α mRNA expression and lung histopathology in rabbits with acute lung injury. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(1): 45-48. doi: Copy

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