Effects of ambroxol hydrochloride on expressions of nuclear factor kappa-B and intercellular adhesion molecule-1 in airway epithelium of smoking rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LIU Xiaomin ,  XU Jianying.

Department of Respiratory Medicine.The First Hospital，Shanxi Medical University.Taiyuan，Shanxi，030001，ChinaCorresponding author：XU Jian-ying，E-mail：xujyty@tom.com;

Corresponding author：

XU Jianying., Email: xujyty@tom.com

Keywords：

Chronic obstructive pulmonary disease; Smoking; Ambroxol; Nuclear factor kappa B; Intercellular adhesion molecule-1

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Objective To explore the anti-inflammatory effects of ambroxol hydrochloride in chronic obstructive pulmonary disease（COPD）.Methods Thirty Wistar rats were randomly divided into three groups，ie.a control group，a smoking group and an ambroxol group.The rats in the smoking and ambroxol groups were exposed to cigarettes smoking for 12 weeks.Ambroxol hydrochloride was administered via intragastric gavage after 4 weeks smoking in the ambroxol group.After 12 weeks，the expiratory airway resistance(Re) and dynamic lung compliance(CLdyn) were measured.The expression levels of nuclear factor kappa B（NF-κB）and intercellular adhesion molecule-1(ICAM-1) in airway epithelium cell were observed by immunohistochemical method.Results Re was increased and CLdyn was decreased significantly in the smoking and ambroxol groups compared with the control group(all P lt;0.01).Re was lower (P lt;0.01) and CLdyn was higher(P lt;0.05) in the ambroxol group than those in the smoking group.B.The level of NF-κB and ICAM-1 in smoking and ambroxol groups were obviously increased compared with the control group (all P lt;0.05)，which was decreased in the ambroxol group compared with the smoking group(both P lt;0.05).C.The expression of NF-κB was positively correlated with ICAM-1 expression in airway epithelial cells（r=0.924，P lt;0.01）.Conclusions Smoking can increase the airway resistance，reduce the lung compliance and increase the expression of NF-κB and ICAM-1 in airway epithelium.Ambroxol hydrochloride can relieve those effects of smoking，which suggested an anti-inflammatory therapeutic role in COPD.

Citation： LIU Xiaomin,XU Jianying.. Effects of ambroxol hydrochloride on expressions of nuclear factor kappa-B and intercellular adhesion molecule-1 in airway epithelium of smoking rats. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(4): 271-273. doi: Copy

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1. Yamamoto Y，Gaynor RB.Therapeutic potential of inhabition of the NF-kappaB pathway in the treatment of inflammation and cancer.J Clin Invest，2001，107：135-142.
2. Noguera A，Sala E，Pons AR，et al.Expression of adhesion molecules during apoptosis of circulating neutrophils in COPD.Chest，2004，125：1837-1842.
3. Rogers DF.Mucus hyper secretion in chronic obstructive pulmonary disease.Novartis Found Symp，2001，234：65-77.
4. 许三林，吴人亮，陈春莲，等.上皮钙粘素在吸烟小鼠呼吸道上皮损伤修复表达的研究.中华结核和呼吸杂志，1999，22：417-419.
5. 关键，郑碧霞，程德云.核因子-κB在慢性阻塞性肺疾病大鼠肺内的表达及泼尼松对其的影响.中国呼吸与危重监护杂志，2008，7：52-56.
6. Di Stefano A，Caramori G，Oates T，et al.Increased expression of nuclear factor-kappaB in bronchial biopsies from smokers and patients with COPD.Eur Respir J，2002，20：556-563.
7. 许建英，杜永成，李冬艳，等.核因子κB、细胞间粘附分子1在吸烟大鼠气道上皮细胞中的表达.中华结核和呼吸杂志，2002，8：493-496.
8. Barnes PJ，Shapiro SD，Pauwels RA.Chronic obstructive pulmonary disease：molecular and cellular mechanisms.Eur Respir J，2003，22：672-688.
9. Malerba M，Ponticiello A，Radaeli A，et al.Effect of twelve-month therapy with oral ambroxol in prevention exacerbations in patients with COPD.Pulm Pharmacol Ther，2004，17：27-34.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of ambroxol hydrochloride on expressions of nuclear factor kappa-B and intercellular adhesion molecule-1 in airway epithelium of smoking rats

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