Effects of Vaporized Perfluorocarbon Inhalation on Histopathology of Lung and Extra-Pulmonary Organs in Rabbits with Acute Lung Injury_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HAN Bingchao ¹ ,  XIE Lixin ¹ , ZHAO Xiaowei ² , HUANG Xian. ²

Department of Respiratory Medicine, Chinese PLA General Hospital. Beijing, 100853, China;

Corresponding author：

XIE Lixin, Email: xielx@301hospital.com.cn

Keywords：

Perfluorocarbon; Acute lung injury; Mechanical ventilation; Histopathology; Multiple organ damage

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effects of vaporized perfluorocarbon( PFC) inhalation on histopathology of lung, small intestine, liver and kidney of acute lung-injured rabbits. Methods Eighteen New Zealand rabbits were randomly divided into 3 groups, ie. a conventional mechanical ventilation( CMV)group, a PFC group, and a control group. The rabbits were mechanical ventilated and intratracheally infused artificial seawater to induce acute lung injury. After ALI was established( PaO2 /FiO2 lt; 200 mm Hg) , the CMV group received CMV for 6 hours. The PFC group received PFC inhalation for 2 hours, and followed by CMV for 4 hours. And the control group was weaned from ventilation. Then they were sacrificed for histopathological measurement of lung, small intestine, liver and kidney. Results The rabbits in the control group died in 15 minutes after discontinuation of ventilation. Vaporized PFC inhalation can obviously improve oxygenation and attenuate the damage of the lung in contrast to CMV. Mild improvement was observed in small intestine, liver and kidney after vaporized PFC inhalation, but without statistical significance. Conclusion Vaporized PFC inhalation can improve oxygenation and attenuate lung injury in histopathology,but have no apparent protective effects on extra-pulmonary organs.

Citation： HAN Bingchao,XIE Lixin,ZHAO Xiaowei,HUANG Xian.. Effects of Vaporized Perfluorocarbon Inhalation on Histopathology of Lung and Extra-Pulmonary Organs in Rabbits with Acute Lung Injury. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(2): 171-175. doi: Copy

1.	钱桂生. 在实践中提高对急性肺损伤和急性呼吸窘迫综合征新概念的认识. 中国呼吸与危重监护杂志, 2002, 1: 199-201.
2.	Gregor T, Schmalisch G, Burkhardt W, et a1 . Aerosolization of perfluorocarbons during mechanical ventilation: an in vitro study.Intensive Care Med, 2003, 29: l354-1360.
3.	黄志坚, 李琦, 王长征, 等. 全氟化碳雾化吸入对兔急性肺损伤肺组织结构及抗炎作用的影响. 中国呼吸与危重监护杂志,2007, 6: 45-48.
4.	樊毫军, 张健鹏, 刘又宁. 吸入全氟化碳治疗急性肺损伤研究进展. 中国呼吸与危重监护杂志, 2006, 5: 63-65.
5.	Koch T, Ragaller M, Haufe D, et al. Perfluorohexane attenuates proinflammatory and procoagulatory response of activated monocytes and alveolar macrophages. Anesthesiology, 2001, 94: 101-109.
6.	Derks CM, Jacoboritz-Derks D. Embolic pneumopathy induced by oleic acid. Am J Pathol, 1977, 87: 143.
7.	Schlag G, Redl H, van Vuuren CJ, et al. Hyperdynamic sepsis in baboonsⅡ : relation of organ damage to severity of sepsis evaluated by a newly developed morphological scoring system. Circ Shock,1992, 38: 253-263.
8.	Zhang XP, Zhang L, Wang Y, et al. Study of the protective effects of dexamethasone on multiple organ injury in rats with severe acute pancreatitis. JOP, 2007, 8: 400-412.
9.	Haeberle HA, Nesti F, Dieteilch HJ, et al. Perllubron reduces lung inflammation in respimtory syncytial virus infection by inhibiting chemokine expression and nuclear factor-kappa B activation. Am J Respir Crit Care Med, 2002, 165: 1433-1438.
10.	刘书盈, 樊毫军, 钱桂生, 等. 全氟化碳腹腔注射对内毒素肺损伤大鼠中性粒细胞肺浸润的抑制作用. 中华结核和呼吸杂志,2006, 29: 674-678.

1. 钱桂生. 在实践中提高对急性肺损伤和急性呼吸窘迫综合征新概念的认识. 中国呼吸与危重监护杂志, 2002, 1: 199-201.
2. Gregor T, Schmalisch G, Burkhardt W, et a1 . Aerosolization of perfluorocarbons during mechanical ventilation: an in vitro study.Intensive Care Med, 2003, 29: l354-1360.
3. 黄志坚, 李琦, 王长征, 等. 全氟化碳雾化吸入对兔急性肺损伤肺组织结构及抗炎作用的影响. 中国呼吸与危重监护杂志,2007, 6: 45-48.
4. 樊毫军, 张健鹏, 刘又宁. 吸入全氟化碳治疗急性肺损伤研究进展. 中国呼吸与危重监护杂志, 2006, 5: 63-65.
5. Koch T, Ragaller M, Haufe D, et al. Perfluorohexane attenuates proinflammatory and procoagulatory response of activated monocytes and alveolar macrophages. Anesthesiology, 2001, 94: 101-109.
6. Derks CM, Jacoboritz-Derks D. Embolic pneumopathy induced by oleic acid. Am J Pathol, 1977, 87: 143.
7. Schlag G, Redl H, van Vuuren CJ, et al. Hyperdynamic sepsis in baboonsⅡ : relation of organ damage to severity of sepsis evaluated by a newly developed morphological scoring system. Circ Shock,1992, 38: 253-263.
8. Zhang XP, Zhang L, Wang Y, et al. Study of the protective effects of dexamethasone on multiple organ injury in rats with severe acute pancreatitis. JOP, 2007, 8: 400-412.
9. Haeberle HA, Nesti F, Dieteilch HJ, et al. Perllubron reduces lung inflammation in respimtory syncytial virus infection by inhibiting chemokine expression and nuclear factor-kappa B activation. Am J Respir Crit Care Med, 2002, 165: 1433-1438.
10. 刘书盈, 樊毫军, 钱桂生, 等. 全氟化碳腹腔注射对内毒素肺损伤大鼠中性粒细胞肺浸润的抑制作用. 中华结核和呼吸杂志,2006, 29: 674-678.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of Vaporized Perfluorocarbon Inhalation on Histopathology of Lung and Extra-Pulmonary Organs in Rabbits with Acute Lung Injury

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content