Mechanism of Pulmonary Fibroblasts Apoptosis Induced by Curcumin_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

GAO Wei ,  ZHANG Deping , CHEN Bi.

Department of Respiratory Medcine, Affiliated Nanjing Drum Tower Hospital of Nanjing University.Nanjing, Jiangsu, 210008, China;

Corresponding author：

ZHANG Deping, Email: dpzhang207@yahoo.com

Keywords：

Curcumin; Pulmonary fibroblast; Pulmonary fibrosis; Apoptosis; Caspase-3

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the proliferation inhibitory effect and to explore the molecular mechanism of curcumin on pulmonary fibroblasts. Methods Fibroblasts derived from lung tissue of patients with idiopathic pulmonary fibrosis ( IPF) was cultured in vitro and incubated with curcumin at different concentrations for different time. Fibroblasts were randomized into 5 groups, ie. a control group and 4 curcumin groups ( intervened by 5, 10, 20, 40 μmol / L curcumin, respectively) . MTT assay was used to determine the inhibitory rate of curcumin on the proliferation of pulmonary fibroblasts. Apoptosis and the Caspase-3 expression of pulmonary fibroblasts were identified by flow cytometry ( FCM) . Variables were compared with One-Way ANOVA. The correlations between variables were analyzed using Pearson’scorrelation coefficient. Results Curcumin inhibited pulmonary fibroblasts proliferation in a dose-dependent and time-dependent manner( r =0. 886, r = 0. 832, respectively, all P lt; 0. 01) . Apoptosis rate of pulmonary fibroblasts in 4 curcumin groups was ( 29. 58 ±2. 13) % , ( 64. 36 ±3. 92) %, ( 72. 98 ±4. 42) % , ( 83. 14 ±2. 51) % , respectively, which was significantly higher than that in the control group[ ( 3. 84 ±1. 88) % , P lt;0. 01] . The positive expression rate of apoptosis-regulating protein caspase-3 was ( 26. 24 ±3. 64) % ,( 44. 87 ±5. 31) % , ( 57. 44 ±4. 23) % , ( 73. 65 ±5. 01) % , respectively, which was significantly higher than that of the control group[ ( 4. 02 ±0. 62) % , P lt; 0. 01] . Conclusions In vitro, curcumin can significantly inhibit proliferation and induce apoptosis of pulmonary fibroblasts of patients with IPF. The mechanism maybe associated with up-regulating expression of Caspase-3.

Citation： GAO Wei,ZHANG Deping,CHEN Bi.. Mechanism of Pulmonary Fibroblasts Apoptosis Induced by Curcumin. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(2): 186-189. doi: Copy

1.	Punithavathi D, Venkatesan N, Babu M. Protective effects of curcumin against amiodarone-induced pulmonary fibrosis in rats. Br J Pharmacol, 2003, 139: 1342-1350.
2.	中华医学会呼吸病学分会. 特发性肺间质纤维化诊断和治疗指南( 草案) . 现代实用医学杂志, 2003, 15: 129-131.
3.	Willis BC, Liebler JM, Luby-Phelps K, et al. Induction of epithelialmesenchymal transition in alveolar epithelia1 cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis.Am J Pathol, 2005, 166: 1321-1332.
4.	Wang R, Ramos C, Joshi I, et al. Human lung myofibroblast-derived inducers of alveolar epithelial apoptosis identified as angiotensin peptides. Am J Physiol, 1999, 277: L1158-L1164.
5.	Tourkina E, Gooz P, Oates JC, et al. Curcumin-induced apoptosis in scleroderma lung fibroblasts: role of protein kinase cepsilon. Am J Respir Cell Mol Biol, 2004, 31: 28-35.
6.	Phan SH. The myofibroblast in pulmonary fibrosis. Chest, 2002 , 122( Suppl 6) : S286-S289.
7.	Liu HL, Chen Y, Cui GH, et al. Curcumin, a potent anti-tumor reagent, is a novel histone deacetylase inhibitor regulating B-NHL cell line Raj i proliferation. Acta Pharmacol Sin, 2005, 26 : 603-609.
8.	张德平, 邱慧, 庄谊. 姜黄素干预对博莱霉素诱导的大鼠肺纤维化的影响. 中华结核和呼吸杂志, 2007, 10: 197-201.
9.	陈碧, 张德平. 姜黄素干预对实验性肺间质纤维化的影响. 中国呼吸与危重监护杂志, 2007, 6: 208-212.
10.	Fan G, Ma X, Wong PY, et al. p53 dephosphorylation and p21 ( Cip1 /Waf1 ) translocation correlate with caspase-3 activation in TGF-beta1-induced apoptosis of HuH-7 cells. Apoptosis, 2004, 9:211-221.

1. Punithavathi D, Venkatesan N, Babu M. Protective effects of curcumin against amiodarone-induced pulmonary fibrosis in rats. Br J Pharmacol, 2003, 139: 1342-1350.
2. 中华医学会呼吸病学分会. 特发性肺间质纤维化诊断和治疗指南( 草案) . 现代实用医学杂志, 2003, 15: 129-131.
3. Willis BC, Liebler JM, Luby-Phelps K, et al. Induction of epithelialmesenchymal transition in alveolar epithelia1 cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis.Am J Pathol, 2005, 166: 1321-1332.
4. Wang R, Ramos C, Joshi I, et al. Human lung myofibroblast-derived inducers of alveolar epithelial apoptosis identified as angiotensin peptides. Am J Physiol, 1999, 277: L1158-L1164.
5. Tourkina E, Gooz P, Oates JC, et al. Curcumin-induced apoptosis in scleroderma lung fibroblasts: role of protein kinase cepsilon. Am J Respir Cell Mol Biol, 2004, 31: 28-35.
6. Phan SH. The myofibroblast in pulmonary fibrosis. Chest, 2002 , 122( Suppl 6) : S286-S289.
7. Liu HL, Chen Y, Cui GH, et al. Curcumin, a potent anti-tumor reagent, is a novel histone deacetylase inhibitor regulating B-NHL cell line Raj i proliferation. Acta Pharmacol Sin, 2005, 26 : 603-609.
8. 张德平, 邱慧, 庄谊. 姜黄素干预对博莱霉素诱导的大鼠肺纤维化的影响. 中华结核和呼吸杂志, 2007, 10: 197-201.
9. 陈碧, 张德平. 姜黄素干预对实验性肺间质纤维化的影响. 中国呼吸与危重监护杂志, 2007, 6: 208-212.
10. Fan G, Ma X, Wong PY, et al. p53 dephosphorylation and p21 ( Cip1 /Waf1 ) translocation correlate with caspase-3 activation in TGF-beta1-induced apoptosis of HuH-7 cells. Apoptosis, 2004, 9:211-221.

Previous Article
Assessment of Registration Quality of Trials Sponsored by China
Next Article
Organ Transplantation and Brain Death Legislation in China: Current Status and Challenges

Chinese Journal of Respiratory and Critical Care Medicine

Mechanism of Pulmonary Fibroblasts Apoptosis Induced by Curcumin

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content