Matrine Inhibits Cell Proliferation and Expression of CTGF and HIF-1αof Human Lung Fibroblast in Normoxia and Hypoxia_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

JIANG Yunyan ,  XIE Min

Department of Respiratory Medicine, West China Hospital, Sichuan University. Chengdu, Sichuan, 610041, ChinaCorresponding Author: XIE Min, E-mail: xiemin@ sciscs. com;

Corresponding author：

XIE Min, Email: xiemin@sciscs.com

Keywords：

Matrine; Human lung fibroblast; Connective tissue growth factor; Hypoxia inducible factor-1α

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effects of matrine on cell proliferation and expression of connective tissue growth factor( CTGF) and hypoxia inducible factor-1α( HIF-1α) of human lung fibroblast ( WRC-5) in normoxia ( 21% O2, 74% N2 , 5% CO2 ) and hypoxia ( 1% O2, 94% N2 , 5% CO2 )conditions. Methods MRC-5 cells were cultured and divided into differrent groups interfered with different dose of Matrine ( final concentration of 0 ～3. 2 mmol / L) in normoxia or hypoxia for 24 h. Cells were divided
into 8 groups according to culture conditions, ie. normoxiagroup( N0 group) , normoxia + matrine 0. 2 mmol / L group( N0. 2 group) , normoxia + matrine 0. 4 mmol / L group( N0. 4 group) , normoxia + matrine 0. 8 mmol / L group( N0. 8 group) , hypoxia group( H0 group) , hypoxia + matrine 0. 2 mmol /L group( H0. 2 group) , hypoxia +
matrine 0. 4 mmol /L group( H0. 4 group) , and hypoxia + matrine 0. 8 mmol / L group( H0. 8 group) . The MTT assay was used to measure the cell proliferation activity. Western-blot assay was used to examine the expression of CTGF and HIF-1α. Results Hypoxia promoted the cell proliferation in all groups( P lt;0. 05) .
Matrine inhibited the proliferation of WRC-5 cells in a concentration-dependent manner in hypoxia or normoxia conditions( P lt;0. 05) . The expression of CTGF andHIF-1αwas lower in normoxia and higher in hypoxia( P lt;0. 01) . Matrine inhibited the expression of CTGF and HIF-1αin a concentration-dependent manner in hypoxia
and normoxia( P lt;0. 05) . Conclusion Matrine can inhibit the cell proliferation and the expression of CTGF and HIF-1αof WRC-5 cells in normoxia and hypoxia in a concentration-dependent manner.

Citation： JIANG Yunyan,XIE Min. Matrine Inhibits Cell Proliferation and Expression of CTGF and HIF-1αof Human Lung Fibroblast in Normoxia and Hypoxia. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(1): 44-47. doi: Copy

1.	Thannickal VJ,Toews GB,White ES,et al . Mechanisms of pulmonary fibrosis. Annu Rev Med,2004,55:395-417.
2.	全国肺心病学组.662例慢性肺源性心脏病尸检病理分析[J].中华病理学杂志,1990,19:22.
3.	洪阁,刘培勋.槐属植物生物碱化学成分及药理作用研究进展.中草药,2005,36:783-788.
4.	黄彤,杨玲,赵龙凤.苦参碱治疗慢性乙型肝炎102例疗效观察.中国药物与临床,2008,8:490-491.
5.	韩海啸,李军祥,江义墩.氧化苦参碱对肝星状细胞增殖抑制和促凋亡作用的影响.中国中西医结合消化杂志,2009,17:41-43.
6.	祝尔健,朴云峰,丁百静,等.苦参碱治疗乙型肝炎后肝硬化近期疗效观察.中国老年学杂志,2008,28:611-612.
7.	Grotendorst GR,Duncan MR. Individual domains of connective tissue growth factor regulate fibroblast proliferation and myofibroblast differentiation. FASEB J,2005,19:729-738.
8.	周艳芳,黄培春,欧阳静萍,等.苦参碱抑制大鼠肺成纤维细胞增殖及其机制.中国现代医学杂志,2006,16:1181-1183.
9.	付松泉,傅松波,杨欣燐,等.苦参碱对博来霉素所致肺间质纤维化大鼠的保护作用.中国临床药理学与治疗学,2007,12:1376-1380.
10.	刘涛,谢敏,王浩凌,苦参碱干预对肺纤维化大鼠转化生长因子 β1和结缔组织生长因子表达的影响中华内科杂志,2007,46:771-773.
11.	Kothapalli D, Grotendorst GR. CTGF modulates cell progressiong in cAMP-arrested NRK fibroblasts.J Cell Physiol,2000,182:119-126.
12.	Karakiulakis G, Papakonstantinou E,Aletras AJ,et al.Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling.J Biol Chem,2007,282:908-915.
13.	Martínez-Sánchez G, Giuliani A.Cellular redox status regulates hypoxia inducible factor-1 activity. Role in tumour development.J Exp Clin Cancer Res,2007,26:39-50.

1. Thannickal VJ,Toews GB,White ES,et al . Mechanisms of pulmonary fibrosis. Annu Rev Med,2004,55:395-417.
2. 全国肺心病学组.662例慢性肺源性心脏病尸检病理分析[J].中华病理学杂志,1990,19:22.
3. 洪阁,刘培勋.槐属植物生物碱化学成分及药理作用研究进展.中草药,2005,36:783-788.
4. 黄彤,杨玲,赵龙凤.苦参碱治疗慢性乙型肝炎102例疗效观察.中国药物与临床,2008,8:490-491.
5. 韩海啸,李军祥,江义墩.氧化苦参碱对肝星状细胞增殖抑制和促凋亡作用的影响.中国中西医结合消化杂志,2009,17:41-43.
6. 祝尔健,朴云峰,丁百静,等.苦参碱治疗乙型肝炎后肝硬化近期疗效观察.中国老年学杂志,2008,28:611-612.
7. Grotendorst GR,Duncan MR. Individual domains of connective tissue growth factor regulate fibroblast proliferation and myofibroblast differentiation. FASEB J,2005,19:729-738.
8. 周艳芳,黄培春,欧阳静萍,等.苦参碱抑制大鼠肺成纤维细胞增殖及其机制.中国现代医学杂志,2006,16:1181-1183.
9. 付松泉,傅松波,杨欣燐,等.苦参碱对博来霉素所致肺间质纤维化大鼠的保护作用.中国临床药理学与治疗学,2007,12:1376-1380.
10. 刘涛,谢敏,王浩凌,苦参碱干预对肺纤维化大鼠转化生长因子 β1和结缔组织生长因子表达的影响中华内科杂志,2007,46:771-773.
11. Kothapalli D, Grotendorst GR. CTGF modulates cell progressiong in cAMP-arrested NRK fibroblasts.J Cell Physiol,2000,182:119-126.
12. Karakiulakis G, Papakonstantinou E,Aletras AJ,et al.Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling.J Biol Chem,2007,282:908-915.
13. Martínez-Sánchez G, Giuliani A.Cellular redox status regulates hypoxia inducible factor-1 activity. Role in tumour development.J Exp Clin Cancer Res,2007,26:39-50.

Chinese Journal of Respiratory and Critical Care Medicine

Matrine Inhibits Cell Proliferation and Expression of CTGF and HIF-1αof Human Lung Fibroblast in Normoxia and Hypoxia

Abstract Full text Figures/Tables Video References Cited by

Format

Content