Objective To study the relationship between smoking as well as smoke cessation and lung inflammation of chronic obstructive pulmonary disease( COPD) , and its possible mechanism. Methods Thirty-two Wistar rats were randomly divided into a control group, a low-dose smoking group, a high-dose smoking group, and a smoke cessation group. The expressions of NF-κB and E-selectin in rats lung were detected by immunohistochemistry and hybridization in situ. Results The mRNA and protein expressions of NF-κB in bronchial endothelial cells of the high-dose smoking group( 0. 43 ±0. 01, 0. 41 ±0. 04) , the lowdose smoking group( 0. 41 ±0. 01, 0. 40 ±0. 01) , and the smoke cessation group( 0. 39 ±0. 01, 0. 37 ±0. 03) , were significantly higher than the control group( 0. 29 ±0. 06, 0. 28 ±0. 06) ( all P lt; 0. 05) , with the high-dose smoking group and the smoke cessation group significantly higher than the low-dose smoking group ( both P lt;0. 05) . The mRNA and protein expressions of E-selectin had the similar pattern with NF-κB. The expressions of E-selectin mRNA and protein were positively correlated with the expression of NF-κB mRNA and protein respectively ( r = 0. 80, r = 0. 89, P lt; 0. 01) . Conclusions Smoking can result in high expressions of NF-κB and E-selectin in a dose dependent manner. Smoke cessation can relieve airway inflammation and is an effective measure for preventing COPD.
Citation：
CHEN Xiaowei,XU Jianying.. Effects of Smoke Cessation on Experssions of Nuclear Factor-κB and E-selectin in Rat Lung Tissues. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(2): 145-149. doi:
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- 1. Li Q, Verma IM. NF-kappaB regulation in the immune system. Nat Rev Immunol, 2002, 2: 725-734.
- 2. 付丽梅, 孙青. E-选择素的研究进展. 医学综述, 2008 , 14: 185-187.
- 3. 曾春芳, 吴亚梅. E-选择素在慢性阻塞性肺疾病大鼠支气管肺组织中的表达. 重庆医学, 2005, 34, 1677-1680.
- 4. 许三林, 吴人亮, 陈春莲, 等. 上皮钙粘附素在吸烟小鼠呼吸道上皮损伤修复中表达的研究. 中华结核和呼吸杂志, 1999, 22 , 417 -419.
- 5. 梁毅, 钟小宁. 戒烟对慢性阻塞性肺疾病肺组织结构和炎症反应的影响. 国际呼吸杂志, 2006, 26, 196 -198.
- 6. Blackwell TS, Christman JW. The role of nuclear factor-kappa B in cytokine gene regulation. Am J Respir Cell Mol Biol, 1997, 17 : 3-9.
- 7. Riise GC, Larsson S, L fdahl CG, et al. Circulating cell adhesion molecules in bronchial lavage and serum in COPD patients with chronic bronchitis. Eur Respir J, 1994, 7: 1673-1677.
- 8. 李庆云, 黄绍光, 万欢英, 等. 不同阶段戒除烟雾刺激对大鼠慢性支气管炎发展过程的影响. 上海医学, 2008 , 31: 208 -210.
- 9. Rutgers SR, Postma DS, ten Hacken NH, et al. Ongoing airway inflammation in patients with COPD who do not currently smoke.Thorax, 2000, 55: 12-18 .
