Immunoliposomes Containing Vasohibin Inhibit Pulmonary Fibrosis in Mice_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Xingsheng ¹ , WU Xueling ² , YANG Xuemei ¹ , ZHAO Yunfeng ³ , XUZhi ² , CUI Shehuai ¹

Department of Respiratory Medicine,Daping Hospital, Third Military Medical University. Chongqing,400042, ChinaCorresponding Author: CUI She-huai, E-mail: cuishehuai@ hotmail. com;

Corresponding author：

Keywords：

Vasohibin; Immunoliposome; Pulmonary fibrosis

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Objective To investigate the effects of immunoliposomes containing vasohibin on pulmonary fibrosis in mice. Methods Liposomes containing vasohibin plasmids were prepared by reverse phase evaporation technique, then combined with VWF antibody to form the immunoliposomes. 30 mice were randomly divided into a control group, a model group, and a vasohibin group. The mice in the model group and the vasohibin group were nasally instilled with bleomysin to induce pulmonary fibrosis. After 3 days, the mice in the vasohibin group were nasally instilled with the immunoliposomes containing vasohibin. The mice were all sacrificed after 14 days. Lung tissue sections were stained by HE and CD31 immunohistochemistry staining. The level of hydroxyproline in lung was measured by colorimetry. Results The results
demonstrated that the vasohibin immunoliposome could markedly decrease angiogenesis with the number of ( 46 ±16) compared to ( 78 ±12 ) , and decrease fibroblast proliferation with ( 0. 84 ±0. 12) μg/mg compared to ( 1. 39 ±0. 23) μg/mg. Conclusion The vasohibin immunoliposome can attenuate pulmonary
fibrosis and provide a novel strategy for clinical application in the future.

Citation： WANG Xingsheng,WU Xueling,YANG Xuemei,ZHAO Yunfeng,XUZhi,CUI Shehuai. Immunoliposomes Containing Vasohibin Inhibit Pulmonary Fibrosis in Mice. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(5): 529-531. doi: Copy

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1. 陈恒屹, 崔社怀. 特发性肺间质纤维化发病机制及治疗进展. 中华肺部疾病杂志( 电子版) , 2009 , 2: 58 -62.
2. 徐佳波, 李燕芹. 肺纤维化和新生血管. 国外医学( 内科学分册) ,2006, 33 : 102-104 .
3. Watanabe K, Hasegawa Y, Yamashita H, et al. Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis. J Clin Invest, 2004, 114: 898 -907.
4. Wakusawa R, Abe T, Sato H, et al. Expression of vasohibin, an antiangiogenic factor, in human choroidal neovascular membranes. Am J Ophthalmol, 2008, 146: 235-243.
5. 赵浩, 王任直, 王菲, 等. 脑靶向基因转运免疫脂质体的制备. 药学学报, 2009, 44: 1285 -1290 .
6. 胡雪, 曾照芳, 陈里里. DEC-205 单抗耦联长循环免疫脂质体的制备及其体外靶向. 生物技术通报, 2008, 5: 154-157 .
7. Bauer SM, Bauer RJ, Liu ZJ, et al. Vascular endothelial growth factor-C promotes vasculogenesis, angiogenesis, and collagen constriction inthree-dimensional collagen gels. J Vasc Surg, 2005, 41 : 699-707.

Chinese Journal of Respiratory and Critical Care Medicine

Immunoliposomes Containing Vasohibin Inhibit Pulmonary Fibrosis in Mice

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