Objective  To observe the levels of malonaldehyde (MDA) , interleukin-8 (IL-8) and tumor necrosis factor-α(TNF-α) in lung tissues of subjects with or without chronic obstructive pulmonary disease (COPD) , and investigate their roles in the the pathogenesis of COPD.
Methods  The content of MDA, IL-8 and TNF-αin lung tissues of smokers with COPD (n=9) , ex-smokers with COPD (n=8) , non-smokers with COPD (n=7) , healthy smokers (n=9) , healthy ex-smokers (n=8) and healthy nonsmokers (n=6) was measured with enzyme-linked immunosorbent assay ( ELISA) and corrected by creatinine.
Results  The levels of MDA, IL-8 and TNF-α in lung tissues of the COPD patients were significantly higher than those in the healthy subjects (P lt;0.05) , which were also significantly higher in the smokers when compared with the non-smokers (P lt;0.05) , whether suffering from COPD or not. In the COPD patients, not the levels of IL-8 but MDA and TNF-αin lung tissues of the smokers were significantly higher than those in the ex-smokers (P lt;0.05) ; whereas in the healthy cases, no statistical significance was revealed between the smokers and the ex-smokers on the levels of MDA and IL-8 in lung tissues except TNF-α( P gt;0.05) .
Conclusion  The abnormal increase of MDA, IL-8 and TNF-αin lung tissues caused by chronic smoking may play an important role in the the pathogenesis of COPD.

Citation： FAN Tao,ZHANG Yu,JIANG Hongli,MIN Jie,WANG Ke,ZHOU Wei,MAO Bing. Levels of Malonaldehyde, Interleukin-8 and Tumor Necrosis Factor-αin Lung Tissues from Patients with and without Chronic Obstructive Pulmonary Disease and Effects of Cigarette Smoking. Chinese Journal of Respiratory and Critical Care Medicine, 2012, 11(3): 218-222. doi: Copy

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