Expression of Integrin-αVβ3 in Vascular Endothelium Cultured in Vitro Under Different Level of Shear Stress_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

MENG Wei , XIAO Zhenghua , ZHANG Eryong .

Department of Cardiovascular Surgery, West China Hospital of Sichuan University, Chengdu 610041, P. R. China;

Corresponding author：

Keywords：

Shear stress; Vascular culture; αVβ3-Integrin; Mean optical density

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Objective To observe the expression of integrin αVβ3 in vascular endothelium cultured in vitro at different time points under different level of shear stress. Methods（1）We established a vascular culture system in vitro which could provide steady flow with different level of shear stress, and tested the flow stability when loading different level of shear stress. （2） A total of 50 rabbits were randomly divided into low shear stress group （5 dyn/cm2, n=25）and normal shear stress group（20 dyn/cm2, n=25）. Rabbits in each group were further randomly divided into five different time points as 2 h, 4 h, 8 h, 16 h and 24 h（n=5 at each time point）. The descending aorta of rabbits were harvested and cultured in the vascular culture system in vitro under different level of shear stress. The expression sites and intensity of αVβ3-Integrin in vascular endothelium were examined at 5 different time points in both groups by immunohistochemical staining. Results The vascular culture system in vitro was stable in providing laminar flow with different level of shear stress required for the experiment. Vascular endothelium expressions of αVβ3-Integrin in the low shear stress group were in high level at all the 5 time points and reached its summit at 16 h, when the mean optical density（MOD）value was （1.995±0.194）×10-2. In the normal shear stress group, the MOD value decreased time-dependently at the 5 time points. The MOD values at 2 h （0.059±0.005）×10-2 and 4 h（0. 049±0.002）×10-2 were significantly higher than those at other time points （P＜ 0.05）. The αVβ3-Integrin MOD values of the low shear stress group were significantly higher than those of the normal shear stress group at all the 5 respective time points （P=0.000）. Conclusion Low shear stress can significantly promote the expression of αVβ3-Integrin while normal shear stress decreases the expression of αVβ3-Integrin in vascular endothelium cultured in vitro.

Citation： MENG Wei,XIAO Zhenghua,ZHANG Eryong .. Expression of Integrin-αVβ3 in Vascular Endothelium Cultured in Vitro Under Different Level of Shear Stress. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2012, 19(2): 172-176. doi: Copy

1.	?2 Shyy JY, Chien S. Role of integrins in endothelial mechanosensing of shear stress. Circ Res, 2002, 91 （9）： 769-775.
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6.	?7 Urbich C, Walter DH, Zeiher AM, et a1. Laminar shear stress upregulates integrin expression： role in endothelial cell adhesion and apoptosis. Circ Res, 2000, 87 （8）： 683-689.
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14.	刘波, 姜宗来, 张炎, 等. 血管体外应力培养系统：一种新的血管生物力学实验模型. 医用生物力学, 2001, 16 （4）：225-230．.
15.	Bleiziffer S, Eichinger WB, Lange R. et a1. regarding article, “long-term outcomes after valve replacement for low-gradient aortic stenosis：impact of prosthesis-patient mismatch”. Circulation, 2006，114 （23）： e627-e628.
16.	胡佳, 刘宸铖, 吴江, 等. 流场压力信号变化对内皮细胞形态和黏附功能的影响. 生物医学工程学杂志, 2008, 25 （6）：1372-1376.
17.	, 116.

1. ?2 Shyy JY, Chien S. Role of integrins in endothelial mechanosensing of shear stress. Circ Res, 2002, 91 （9）： 769-775.
2. ?3 Osborn EA, Rabodzey A, Dewey CF Jr, et a1. Endothelial actin cytoskeleton remodeling during mechanostimulation with fluid shear stress. Am J Physiol Cell Physiol, 2006, 290 （2）： C444-C452.
3. ?4 Witmer AN, van Blijswijk BC, van Noorden CJ, et a1. In vivo angiogenic phenotype of endothelial cells and pericytes induced by vascular endothelial growth factor-A. J Histochem Cytochem, 2004, 52 （1）： 39-52.
4. ?5 Orr AW, Ginsberg MH, Shattil SJ, et a1. Matrix-specific suppression of integrin activation in shear stress signaling. Mol Biol Cell, 2006, 17 （11）： 4686-4697.
5. ?6 Sho E, Komatsu M, Sho M, et a1. High flow drives vascular endothelial cell proliferation during flow-induced arterial remodeling associated with the expression of vascular endothelial growth factor. Exp Mol Pathol, 2003, 75 （1）： 1-11.
6. ?7 Urbich C, Walter DH, Zeiher AM, et a1. Laminar shear stress upregulates integrin expression： role in endothelial cell adhesion and apoptosis. Circ Res, 2000, 87 （8）： 683-689.
7. ?8 张步春, 王安才, 李利芳, 等. 流体切应力对人内皮细胞迁移和整合素基因表达的影响. 中国微循环, 2008, 12 （2）：101-.
8. ?9 Ando J, Kamiya A. Blood flow and vascular endothelial cell function. Front Med Biol Eng, 1993, 5 （4）： 245-264.
9. 姜宗来. 心血管生物力学研究的新进展. 医用生物力学, 2010, 25 （5）：313-315, 351．.
10. Nerem RM, Alexander RW, Chappell DC, et a1. The study of the influence of flow on vascular endothelial biology. Am J Med Sci, 1998, 316 （3）： 169-175.
11. Fung YC. Biodynamics： Circulation. New York：Springer-Verlag Inc, 1984. 136-142.
12. Moser M, Legate KR, Zent R, et a1.The tail of integrins,talin and kindlins. Science, 2009, 324 （5929）： 895-899.
13. Kim M, Carman CV, Springer TA. Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Science, 2003, 301 （5640）： 1720-1725.
14. 刘波, 姜宗来, 张炎, 等. 血管体外应力培养系统：一种新的血管生物力学实验模型. 医用生物力学, 2001, 16 （4）：225-230．.
15. Bleiziffer S, Eichinger WB, Lange R. et a1. regarding article, “long-term outcomes after valve replacement for low-gradient aortic stenosis：impact of prosthesis-patient mismatch”. Circulation, 2006，114 （23）： e627-e628.
16. 胡佳, 刘宸铖, 吴江, 等. 流场压力信号变化对内皮细胞形态和黏附功能的影响. 生物医学工程学杂志, 2008, 25 （6）：1372-1376.
17. , 116.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Expression of Integrin-αVβ3 in Vascular Endothelium Cultured in Vitro Under Different Level of Shear Stress

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