TANG Hua , XU Zhifei , LIU Changsheng , et al .
  • 1.Department of Thoracic and Cardiovascular Surgery, Shanghai Changzheng Hospital,Shanghai 200003，P.R.China ;;
  • 2.East China University of Science and Technology, Shanghai 200011, P.R.China;
Porous phosphate calcium; Polylactide-co-glycolide/hydroxyapatite; Tissue engineering; Degradabality experiment in vitro; Degradabality experiment in vivo
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Objective Choose polylactide-co-glycolide/hydroxyapatite (PLGA/HA) and porous phosphate calcium (PPC) as the object that we will study, compare their degradabality and choose one as a suitable scaffold for rib reconstruction. Methods All the experiments were divided into PLGA/HA group and CPC group. Degradabality experiment in exvivo: put the two scaffold which have the same size into 0.9% NaCl, keep sterile, then put the container into warm cage，get out and weigh them in 2, 4, 8, 12 and 24 weeks, compare the different speed of the two scaffold. Degradability experiment in vivo: put the two scaffold which have the same size under the skin of the rabbit, and weigh them in 2, 4, 8, 12 and 24 weeks, the tissue around the scaffold was examinzed by HE and the scaffold was examined by electron scanning microscope. Results Micro-CT and Scanning electron microscopy shows that CPC group had better structure （1101.2228±0.6184 mg/ccm vs. 1072.5523±0.7442 mg/ccm）and porosity（70.26％±0.45％ vs.72.82％±0.51％）than PLGA/HA group; The result of degradabality experiment in vitro shows that the speed of the two scaffolds was slow. It is at 24 weeks that the degradability is obvious,and the PLGA/HA group degraded a lot which was 60%. The result of degradabality experiment in vivo shows that the speed of degradabality of PLGA/HA group was faster than that is in the 0.9% Nacl, also was faster than that of CPC group which was 96%.The reponse of tissue around the PLGA/HA was more sever than that of CPC group which is in favour of the growth of cells. Conclusion As for the reconstruction of large defect of rib, CPC is more suitable than PLGA/HA.

Citation： TANG Hua,XU Zhifei,LIU Changsheng,et al .. The Preparation of Two Scaffolds for Tissue Engineering Rib and Comparison of Their Degradability. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2009, 16(4): 287-291. doi: Copy

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