IMPROVEMENT OF SPINAL CORD SEMI-TRANSECTED AND LUMP DEFECT MODEL BY QUANTITATE EMITRANSECTEDBLADE IN RATS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

HU Wei ^1,2 , GUAN Fangxia ^3,4 , SUN Jianrui ² , TANG Youjia ¹ , YANG Feng ¹ , LI Yuan ² , YANG Bo ²

1Department of Neurosurgery, the First People’s Hospital of Jiujiang, Jiujiang Jiangxi, 332000, P.R.China;;
2Department ofNeurosurgery, the First Affiliated Hospital, Zhengzhou University;;
3Department of Bioengineering, Zhengzhou University;;
4Henan Academy of Medical Sciences.;

Corresponding author：

Keywords：

Semi-transected spinal cord injury; Quantitative semi-transected blade; Rat

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Objective To investigate the effect of quantitative semi-transected blade on the improvement of spinal cord semi-transected and lump defect model. Methods Forty-eight male Sprague Dawley rats (weighing 220-250 g) were divided into the experimental group (n=24) and control group (n=24). The spinal cord semi-transected and lump defect model was made by self-made quantitative semi-transected blade in the experimental group, and by ophthalmic scalpel in the control
group. Then, the complications were observed; the electrophysiological results were detected before modeling and at 21 days after modeling; the histological changes at margin of lump defect were observed at 6 hours, 5 days, and 28 days; Basso, Beattie, and Bresnahan (BBB) scores were detected at 1, 3, 5, 7, 14, 21, 28, 35, 42, 56, and 84 days after modeling. Results There was significant difference in the mortality between the experimental group (0) and the control group (26.67%) (P=0.028). Electrophysiological examination: there was no significant difference in latency and ampl itude of motor evoked potentials (MEP) and sensory evoked potentials (SEP) between 2 groups at preoperation (P gt; 0.05); at 21 days after operation, latencies of MEP and SEP increased and the amplitude decreased in the control group, showing significant differences when compared with those in the experimental group and the preoperative values (P lt; 0.05), but no significant difference was seen between preoperation and postoperation in the experimental group (P gt; 0.05). Histological examination: in the control group, small hematoma could be observed at normal side at 6 hours after modeling, increased spaces of spinal tissue and perineural invasion were observed at 5 days, and small cavity formed without normal motoneurons at 28 days in the margin of lump defect. In the experimental group, no small hematoma could be observed at 6 hours after modeling, no inreversible injury of neuron and small cavity were observed at 5 days, and normal motoneurons were observed without small cavity at 28 days in the margin of lump defect.BBB scores: except the scores between experimental group and control group at affected side (P gt; 0.05), there were significant differences between groups, and between normal side and affected side for intragroup (P lt; 0.05). Conclusion Semi-transected and lump defect model could be set up successfully by self-made quantitate semi-transected blade, procedure is repetitive and the model is stable. This model is an ideal model for semi-transected spinal cord injury.

Citation： HU Wei,GUAN Fangxia,SUN Jianrui,TANG Youjia,YANG Feng,LI Yuan,YANG Bo. IMPROVEMENT OF SPINAL CORD SEMI-TRANSECTED AND LUMP DEFECT MODEL BY QUANTITATE EMITRANSECTEDBLADE IN RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(4): 416-420. doi: Copy

1.	张绍东, 郝淑煜, 历俊华, 等. 聚乳酸-乙醇酸支架移植对脊髓半横断损伤大鼠后肢运动能力和运动诱发电位的影响. 中国卒中杂志, 2010, 5(4): 286-290.
2.	边涛, 朱涛, 刘通, 等. 大鼠脊髓半切损伤后外源性NEP1-40对神经中丝NF200表达的影响. 山东医药, 2010, 50(31): 29-31.
3.	薛辉, 陈东, 张秀英, 等. 化学去细胞肌肉促进大鼠脊髓半横断后的轴突再生. 西安交通大学学报: 医学版, 2009, 30(2): 154-158.
4.	赵伟, 章为, 周雪, 等. 大鼠脊髓半横断损伤对脊髓versican、CD44表达的影响. 解剖学杂志, 2009, 32(5): 654-656.
5.	倪炜, 李云枚, 关宇光, 等. 猴脊髓半横断模型的制作及后肢功能评价. 四川大学学报: 医学版, 2005, 36(3): 328-330.
6.	曾琳, 李民, 刘媛, 等. 神经干细胞与原浆型星形胶质细胞联合移植对大鼠损伤脊髓轴浆运输功能的修复作用. 中国脊柱脊髓杂志, 2008, 18(4): 298-301.
7.	Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma, 1995, 12(4): 1-21.
8.	Lim JH, Jung CS, Byeon YE, et al. Establishment of a canine spinal cord injury model induced by epidural balloon compression. J Vet Sci, 2007, 8(1): 89-94.
9.	杨建华, 陈杰, 李长德. 大鼠脊髓全横断模型的研究及其评价. 黑龙江医药科学, 2006, 29(1): 1-3.
10.	Gaviria M, Haton H, Sandillon F, et al. A mouse model of acute ischemic spinal cord injury. J Neurotrauma, 2002, 19(2): 205-221.
11.	郭涛, 何明生, 王廷华. 半横断模型与脊髓损伤修复. 临床医学工程, 2008, 15(12): 72-74.
12.	Kobayashi NR, Fan DP, Giehl KM, et al. BDNF and NT-4/5 prevent atrophy of rat rubrospinal neurons after cervical axotomy, stimulate GAP-43 and Talpha1-tubulin mRNA expression, and promote axonal regeneration. J Neurosci, 1997, 17(24): 9583-9595.
13.	Cai J, Ziemba KS, Smith GM, et al. Evaluation of cellular organization and axonal regeneration through linear PLA form implants in acute and chronic spinal cord injury. J Biomed Mater Res A, 2007, 83(2): 512-520.
14.	Moore MJ, Friedman JA. Multiple-channel scaffolds to promote spinal cord axon regeneration. Biomaterials, 2006, 27(3): 419-429.
15.	卢曼鹏, 权正学, 刘渤, 等. 小鼠脊髓损伤标准化重物打击模型的制备及评价. 中国修复重建外科杂志, 2008, 22(8): 933-938.
16.	王熠钊, 徐江, 许涛, 等. 光化学法诱导猫不完全脊髓损伤动物模型的建立. 中华物理医学与康复杂志, 2010, 32(3): 161-165.
17.	茅磊, 王汉东. 脊髓损伤后神经炎症反应的研究现状. 中华神经医学杂志, 2009, 8(12): 1291-1293.
18.	Yu P, Huang L, Zou J, et al. Immunization with recombinant Nogo-66 receptor (NgR) promotes axonal regeneration and recovery of function after spinal cord injury in rats. Neurobiol Dis, 2008, 32(3): 535-542.
19.	Ramon-Cueto A, Cordero MI, Santos-Benito FF, et al. Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia. Neuron, 2000, 25(2): 425-435.
20.	乔晓俊, 杨波, 关方霞, 等. 人脂肪间充质干细胞静脉移植修复脊髓损伤: 相关因子及行为学评价. 中国组织工程研究与临床康复, 2010, 14(40): 7458-7461.

1. 张绍东, 郝淑煜, 历俊华, 等. 聚乳酸-乙醇酸支架移植对脊髓半横断损伤大鼠后肢运动能力和运动诱发电位的影响. 中国卒中杂志, 2010, 5(4): 286-290.
2. 边涛, 朱涛, 刘通, 等. 大鼠脊髓半切损伤后外源性NEP1-40对神经中丝NF200表达的影响. 山东医药, 2010, 50(31): 29-31.
3. 薛辉, 陈东, 张秀英, 等. 化学去细胞肌肉促进大鼠脊髓半横断后的轴突再生. 西安交通大学学报: 医学版, 2009, 30(2): 154-158.
4. 赵伟, 章为, 周雪, 等. 大鼠脊髓半横断损伤对脊髓versican、CD44表达的影响. 解剖学杂志, 2009, 32(5): 654-656.
5. 倪炜, 李云枚, 关宇光, 等. 猴脊髓半横断模型的制作及后肢功能评价. 四川大学学报: 医学版, 2005, 36(3): 328-330.
6. 曾琳, 李民, 刘媛, 等. 神经干细胞与原浆型星形胶质细胞联合移植对大鼠损伤脊髓轴浆运输功能的修复作用. 中国脊柱脊髓杂志, 2008, 18(4): 298-301.
7. Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma, 1995, 12(4): 1-21.
8. Lim JH, Jung CS, Byeon YE, et al. Establishment of a canine spinal cord injury model induced by epidural balloon compression. J Vet Sci, 2007, 8(1): 89-94.
9. 杨建华, 陈杰, 李长德. 大鼠脊髓全横断模型的研究及其评价. 黑龙江医药科学, 2006, 29(1): 1-3.
10. Gaviria M, Haton H, Sandillon F, et al. A mouse model of acute ischemic spinal cord injury. J Neurotrauma, 2002, 19(2): 205-221.
11. 郭涛, 何明生, 王廷华. 半横断模型与脊髓损伤修复. 临床医学工程, 2008, 15(12): 72-74.
12. Kobayashi NR, Fan DP, Giehl KM, et al. BDNF and NT-4/5 prevent atrophy of rat rubrospinal neurons after cervical axotomy, stimulate GAP-43 and Talpha1-tubulin mRNA expression, and promote axonal regeneration. J Neurosci, 1997, 17(24): 9583-9595.
13. Cai J, Ziemba KS, Smith GM, et al. Evaluation of cellular organization and axonal regeneration through linear PLA form implants in acute and chronic spinal cord injury. J Biomed Mater Res A, 2007, 83(2): 512-520.
14. Moore MJ, Friedman JA. Multiple-channel scaffolds to promote spinal cord axon regeneration. Biomaterials, 2006, 27(3): 419-429.
15. 卢曼鹏, 权正学, 刘渤, 等. 小鼠脊髓损伤标准化重物打击模型的制备及评价. 中国修复重建外科杂志, 2008, 22(8): 933-938.
16. 王熠钊, 徐江, 许涛, 等. 光化学法诱导猫不完全脊髓损伤动物模型的建立. 中华物理医学与康复杂志, 2010, 32(3): 161-165.
17. 茅磊, 王汉东. 脊髓损伤后神经炎症反应的研究现状. 中华神经医学杂志, 2009, 8(12): 1291-1293.
18. Yu P, Huang L, Zou J, et al. Immunization with recombinant Nogo-66 receptor (NgR) promotes axonal regeneration and recovery of function after spinal cord injury in rats. Neurobiol Dis, 2008, 32(3): 535-542.
19. Ramon-Cueto A, Cordero MI, Santos-Benito FF, et al. Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia. Neuron, 2000, 25(2): 425-435.
20. 乔晓俊, 杨波, 关方霞, 等. 人脂肪间充质干细胞静脉移植修复脊髓损伤: 相关因子及行为学评价. 中国组织工程研究与临床康复, 2010, 14(40): 7458-7461.

Chinese Journal of Reparative and Reconstructive Surgery

IMPROVEMENT OF SPINAL CORD SEMI-TRANSECTED AND LUMP DEFECT MODEL BY QUANTITATE EMITRANSECTEDBLADE IN RATS

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