Objective To investigate the ability of plateletrich plasma(PRP) combined with cells and artificial bone in accelerating the repair of bone defect. Methods The marrow stromal stem cells (MSCs) of rabbit were cultured and induced into the osteoblast-like cells in vitro. PRP was produced with low-density twice centrifugations. Forty-eight New Zealand rabbits were made 1.2 cm bilateral radius defect models and divided into 4 groups averagely at random: group A(left:PRP／MSCs／β-tricalcium phosphate(β-TCP), right: MSCs／β-TCP), group B (left:autoradius, right: PRP／MSCs／β-TCP)； group C (left:autoradius,right: MSCs／β-TCP), and group D(left:PRP／β-TCP; right:β-TCP). At 2, 6 and 12 weeks after operation， the repair of bone defect was evaluated by the generalobservation, histology, biomechanics and histomorphology. Results There was a stable platelet concentration in PRP and it was about 5.45±0.23 times of whole blood. In the aspect of bone bridge and conture of the defects, at 2 and 6 weeks, PRP／MSCs／β-TCP and MSCs／β-TCP displayed asimilar outcome and were less than auto in general sample and X-ray;at12 weeks，PRP／MSCs／β-TCP was similar to autoradius and better than MSCs／β-TCP.in the aspect of quantity and quality of bone formation，histology showed that PRP／MSCs／β-TCP and autoradius were better than MSCs／β-TCP（P＜0.05），and there was nosignificantdifference between PRP／MSCs／β-TCP and autoradius（P＞0.05）. At 2 and 6 weeks，there was no significant difference between PRP／β-TCP and β-TCP（P＞0.05）。At 12 weeks，PRP／β-TCP was better than β-TCP（P＜0.05）. In the aspect of intensity f bone formation，at 6 and 12 weeks，PRP／MSCs／β-TCP and autoradiuswere better than MSCs／β-TCP（P＜0.05）. At 6 weeks，autoradius was better than PRP／MSCs／β-TCP（P＜0.05）. At 12 weeks，there was no significant difference between PRP／MSCs／β-TCP and auto（P＞0.05）. PRP／TCP and β-TCP had no significant difference at 12 weeks（P＞0.05）. Conclusion PRP／MSCs／β-TCP demonstrated excellent ability of forming bone in experiment. PRP was most likely to accelerate the repair of bone defect through increasing the activity of proliferation and differentiationof MSCs and osteoblasts.

Citation： LI Sibo,ZHANG Changqing,YUAN Ting,et al.. OSTEOGENIC POTENTIAL OF PLATELETRICH PLASMA COMBINED WITH CELLS ANDARTIFICIAL BONE. Chinese Journal of Reparative and Reconstructive Surgery, 2007, 21(1): 58-64. doi: Copy

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