Objective To investigate the role of transforming growth factorβ3 (TGF-β3) on the amylase secretion of rat submandibular gland cells(RSGCs).Methods The RSGCs were cultured and identified. The expressions of CK 8.13, S100 and Vimentin in the RSGCs were examined by immunohistochemical staining. The experimental group was divided into 5 groups according to differentconcentrations of TGF-β3 (0.5, 1.0, 5.0, 10.0 and 25.0 ng/ml) and no TGF-β3 culture was used as control group. The effects ofTGF-β3 on the cell proliferation and amylase secretion were examined at the24th, the 48th, the 72nd and the 96th hour. MTT colorimetric method was used to estimate vital force of culture cells. Amylase protein was assayed by autobiochemistry equipment and Western blotting.Results The RSGCs were stained positively for CK 8.13 and S-100, but negatively for Vimentin. There were no significant differences in absorbency between the experimental groups and the control group(P gt;0.05). Compared with the control group,TGF-β3 at concentrations of 0.5-10.0 ng/ml significantly stimulated the amylase secretion of RSGCs after 72 and 96 hours(P lt;0.01). But high concentration of TGF-β3 (25.0ng/ml) showed no stimulation. Western blotting demonstrated that the cultured RSGCs and submandibular gland had the same band of amylase electrophoresis.Conclusion TGF-β3 can stimulate RSGCs to differentiate and to secrete amylase, but TGF-β3 has no effect on proliferation ofRSGCs.

Citation： ZHU Yaomin,ZHOU Qing,WANG Yuxin.. ROLE OF TRANSFORMING GROWTH FACTOR β3 ON AMYLASE SECRETION OF SUBMANDIBULAR GLAND CELLS IN RAT. Chinese Journal of Reparative and Reconstructive Surgery, 2004, 18(6): 490-493. doi: Copy

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