• Key Laboratory of Transplant Engineering and Immunology of Ministry of Public Health: Institute of Reparative and Reconstructive Surgery·Laboratory of Tissue Engineering;;
  • West China Hospital;;
  • Sichuan University. Chengdu Sichuan;;
  • P. R. China 610041;;
Tissue engineering; Osteoblast Bio derived material; Pluronic F-127
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OBJECTIVE: To investigate the effects of three-dimentional culture in bioderived material modified by Pluronic F-127 on the growth and function of rabbit periosteal osteoblast in vitro. METHODS: Bio-derived materials were from fresh pig ribs and were modified by Pluronic F-127. Then rabbit periosteal osteoblasts were cultured in bio-derived materials(group A), in the modified bio-derived materials(group B) and on the plastic surfaces as a control (group C), respectively. During a 7-day period, the status of growth, cell viability and alkaline phosphatase(ALP) activity were measured. RESULTS: Osteoblasts attached, elongated and grew well on the modified bio-derived materials. There were no significant difference in osteogenesis and ALP activity between group A and group B(P  gt; 0.05). The osteogenesis and ALP activity in groups A, B were less than those in group C (P  lt; 0.01). CONCLUSION: Pluronic F-127 can be used for a carrier for bioactive factors to modify bio-derived material.

Citation： REN Yong xin,YANG Zhi ming,LI Xiu qun,et al.. EXPERIMENTAL STUDY OF MODIFIED BIO-DERIVED MATERIAL BY PLURONIC F-127 IN VITRO. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(1): 52-55. doi: Copy

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