Export PDF Favorites Scan Get Citation

Objective To investigate the experimental condition and mechanism of differentiation of human umbilical cord blood derived mesenchymal stem cells(hUCB-MSC)into neuron-like cells induced by recombined human epidermal growth factor (rhEGF) and taurine in vitro.Methods hUCB-MSC were primary cultured in Dulbecco prime;s modified Eagle's medium/F12 (DMEM/F-12)which supplemented with 105U/L penicillin G, 100 mg/L streptomycin sulfate, 10% fetal bovine serum (FBS),5% autologous plasma,4 mmol Lglutamine, 30 ng/ml rhEGF.The DMEM/F-12 medium was replaced by taurine medium after 3 passages.The expression of surface antigen CD90,CD29,CD34,CD44 and CD45 were detected by flow cytometry;the expression of neuron specific enolase,rhodopsin and nestin were investigated by immunocytochemistry. The statistical method was chi square test.Results Morphologically similar to bonemarrow MSC,hUCB-MSC became attached cells after the first 5 to 7 days in culture,and reached 80% to 90% confluent after 3 to 4 weeks. Growth accelerated after passage. hUCB-MSC were positive for CD29,CD44 and CD90 but negative for CD34 and CD45. After taurine induction, 2515/3120 cells expressed NSE, 1168/3175 cells expressed rhodopsin and 903/3050 cells expressed nestin while only 234/2965 cells expressed NSE in the control group(P<0.01).Conclusion rhEGF and taurine can induce hUCB-MSC differentiating into neuronlike or rhodopsin positive cells.

Citation: 金玮,邢怡桥,杨安怀,杨燕宁,艾明. Recombined human epidermal growth factor and taurine induce human umbilical cord blood derived mesenchymal stem cells differentiating into neuron-like cells. Chinese Journal of Ocular Fundus Diseases, 2009, 25(4): 261-264. doi: Copy

Copyright © the editorial department of Chinese Journal of Ocular Fundus Diseases of West China Medical Publisher. All rights reserved