• Department of Opthalmo lo gy, Affiliated First Guangzhou Municipal People′;
  • s Hospital, Guangzhou Medical C ollege, Guangdong 510180, China;
Estrogens/physiology; Reperfusion injury; Glu tamic acid; Electroretinography; Animal experimentation
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Objective:To observe the effect of  beta;estradiol on gluta mate concentration in rabbits prime; retinae injured by ischemic reperfusion. Methods:Twenty r abbits ware randomly divided into two groups, the control group and the treatmen t group, with 10 rabbits in each group. Before examined by binocular flash elect roretinography (FERG), retinal ischemic reperfusion (RIR) model was induced in t h e right eyes of all the rabbits by increasing intraocular pressure to 120 mm Hg for 60 minutes; the left eyes were as the control eyes. The rabbits were hypoder mically injected with  beta;estradiol (0.1 mg/kg) in treatment group and with phys i ological saline in the control group 2 hours before ischemia. The results of FER G of the right eyes in both of the 2 groups 0, 4, 8, and 24 hours after reperfus ion were record respectively and were compared with the results of FERG before r eperfusion. The retina tissue was collected after the last time of FERG. The con c entration of glutamate was detected by Hitachi L8800 amino acid analyzer. Results:In the right eyes in both of the 2 groups, the result of F ERG showed a beeli ne just after reperfusion. There was no significant difference of awave amplit u de between the 2 groups (t=1.357, 0.798, 0.835; P gt;0.05); the b wave amplitudes i n experimental group were much higher than those in the control group (t=4.447, 2.188, 3.106; P lt;0.01). The concentration of glutamate in retina was (0.265 plusmn;0.014) g/L in the right eyes and (0.207 plusmn;0.013) g/L in the left eyes in the control group, and (0.231 plusmn;0.007) g/L in the right eyes and (0.203 plusmn;0 .014) g/L in the le ft eyes in the treatment group; the difference between the 2 groups was signific ant (F=50.807, P=0.000). There was statistical difference between righ t and left eyes both in the 2 groups and the significant difference of the right eyes betw een the two groups was also found (P=0.000); there was no statistical diffe rence of the left eyes between the 2 groups (P＝0.505). Conclusion: beta;-estradiol may prevent the increase of the concentration of glutamate in retina induced by RIR to protect retinal tissue.

Citation： ZHU Yuanjun,JIN Min,GAO Zongyin,e t al.. Effect of β-estradiol on glutamate concentration in retina injured by ischemic reperfusion. Chinese Journal of Ocular Fundus Diseases, 2007, 23(5): 321-323. doi: Copy

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