目的:探讨贯叶连翘提取物对百草枯诱导的大鼠肺纤维化模型的干预作用及机制。方法:20只Sprague-dawly大鼠随机等分为4组:百草枯组,贯叶连翘提取物组,百草枯+贯叶连翘提取物组和对照组。百草枯液按80mg/kg一次性灌胃，贯叶连翘提取物按400mg/kg灌胃，连用3d,对照组仅用生理盐水。于21d处死后取肺脏行HE染色鉴定,并用碱水解法测羟脯氨酸含量,同时以八木国夫荧光法测定肺组织脂质过氧化产物,盐酸羟胺法测定组织总超氧化物歧化酶的酶活力。结果:百草枯染毒动物可致肺纤维化,羟脯氨酸和丙二醛含量明显增高。百草枯+贯叶连翘提取物组与百草枯组比较,第21天时肺纤维化减轻,羟脯氨酸和丙二醛含量减少(P lt;0.05),与对照组相比总超氧化物歧化酶无明显变化(P gt;0.05)。结论:贯叶连翘提取物对百草枯诱导的大鼠肺纤维化模型有抑制作用,其机制可能与抑制脂质过氧化有关。
Citation：
HE BIN,DU XIAOdong,CAO Yu.. Effects of Hypericum Perforatum L on Paraquatinduced Pulmonary Fibrosis in Rats. West China Medical Journal, 2009, 24(6): 1473-1475. doi:
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魏薇,何庆.贯叶连翘提取物对百草枯所致的急性肺微血管内皮细胞损伤的保护作用[J].四川大学学报(医学版),2006,37(3):434-437.
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CZINNER E, HAGYMA′SI K, BLA′ZOVICS A, et al. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation[J]. J Ethnopharmacol,2001,77:31-35.
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CAKIR A, MAVI A, YILDIRIM A, et al. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L by activityguided fractionation[J]. J Ethnopharmacol,2003,87(1):73-83.
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RUSSEL P B, JAMES D C． Oxidative stress in airways: Is there a role for extracelular superoxide dismutase?[J]Am J Respir Crit Care Med,2002,166(12):38-43.
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- 1. 魏薇,何庆.贯叶连翘提取物对百草枯所致的急性肺微血管内皮细胞损伤的保护作用[J].四川大学学报(医学版),2006,37(3):434-437.
- 2. 高燕,雍政,黄英,等.百草枯建立肺纤维化动物模型的实验研究[J].空军总医院学报,2003,19(4):201-202.
- 3. ORITO K, SUZUKI Y, MATSUDA H, et al. Chymase is activated in the pulmonary inflammation and fibrosis induced by paraquat in hamsters[J]. Tohoku J Exp Med,2004,203(4):287-294.
- 4. ADACHI J, ISHII K, TOMITA M, et al. Consecutive administration of paraquat to rats induces enhanced cholesterol peroxidation and lung injury[J]. Arch Toxicol,2003,77(6):353-357.
- 5. CZINNER E, HAGYMA′SI K, BLA′ZOVICS A, et al. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation[J]. J Ethnopharmacol,2001,77:31-35.
- 6. CAKIR A, MAVI A, YILDIRIM A, et al. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L by activityguided fractionation[J]. J Ethnopharmacol,2003,87(1):73-83.
- 7. RUSSEL P B, JAMES D C． Oxidative stress in airways: Is there a role for extracelular superoxide dismutase?[J]Am J Respir Crit Care Med,2002,166(12):38-43.
