Change of Immunologic Gene Expression in Cases of Colorectal Cancer with Liver Metastasis by DNA Microarray_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAN Dengguo , WANG Guodong , CHENG Haiyu

Department of Colorectal Anus Surgery， Affiliated Hospital of Guiyang Medical College， Guiyang 550004， Guizhou Province， China;

Corresponding author：

Keywords：

cDNA microarray; Liver metastasis of colorectal cancer; Immunologic gene

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Objective 　To investigate the change of immunologic gene expression in cases of colorectal cancer with liver metastasis.
Methods 　The total RNAs were extracted from tumor tissues of original lesions in 16 patients with colorectal cancer， DNA microarray was used to examine the change of immunologic gene expression in colorectal cancer patients with or without liver metastasis.
Results 　Compared with samples without liver metastases， the expressions of 11 immunologic genes obviously down-regulated in the tumor tissues of colorectal cancer patients with liver metastasis， including：carboxypeptidase D；Fc fragment of IgE， high affinityⅠreceptor for gamma polypeptide；Fc fragment of IgG， low affinityⅢa receptor （CD16a）；free fatty acid receptor 2；interleukin 2 receptor gamma；protein tyrosine phosphatase receptor type C；complement factor B；major histocompatibility complex， classⅡ， DM alpha；major histocompatibility complex， classⅡ， DM beta；major histocompatibility complex， classⅡ， DQ alpha 1；granzyme B. The functions involved the growth and activation of immunologic cell， signal transduction， cell apoptotic， cell factors， receptors， complement， apoptotic， and immunogenicity of tumor cell.
Conclusions 　Down-regulation of a various of immunologic gene expression in colorectal cancer patients with liver metastasis inhibits the function of immunology， and tumor cells escaped the destruction of immunology system results in metastasis.

Citation： YAN Dengguo,WANG Guodong,CHENG Haiyu. Change of Immunologic Gene Expression in Cases of Colorectal Cancer with Liver Metastasis by DNA Microarray. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(11): 1182-1186. doi: Copy

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1. 孙立峰，丁凌，范钰，等. 结直肠肿瘤肝转移基础研究概况[J]. 实用肿瘤杂志， 2007， 22(3)：198-200.
2. 屈伸，刘志国. 分子生物学实验技术[M]. 北京：化学工业出版社， 2007：364-398.
3. 邢戌健，贾户亮，张磊，等. 基因芯片技术在肝细胞癌候选诊断指标筛选中的应用[J]. 中国普外基础与临床杂志， 2007， 14(1)：23-27.
4. 张红，楚胜华，冯东福，等. 应用基因芯片筛选不同胶质瘤细胞相关基因[J]. 中华实验外科杂志， 2012， 29(2)：253-255.
5. Boltze C. Loss of maspin is a helpful prognosticator in colorectal cancer：a tissue microarray analysis[J]. Pathol Res Pract， 2005， 200(11-12)：783-790.
6. 文坤明，曾庆良，冯国丽，等. 结直肠癌患者淋巴结微转移与其免疫功能的关系[J]. 中国普外基础与临床杂志， 2009， 16(11)：890-894.
7. Ge W， Hu H， Zheng S. High osteopontin and low SPARC-like 1 co-expression in colorectal liver metastasis were identified by integrated analysis of cytoband and microarray[C]. American Association for Cancer Research Annual Meeting：Proceedings. 2007 Apr 14-18；Los Angeles， CA. Philadelphia (PA)：AACR；2007. Abstract nr 4701.
8. 丁友成，张辉，钟明安，等. 基因芯片技术筛选大肠癌肝转移差异表达基因[J]. 南京医科大学学报(自然科学版)， 2008， 28(4)：476-479.
9. Le GT， Abbenante G， Fairlie DP. Profiling the enzymatic properties and inhibition of human complement factor B[J]. J Biol Chem， 2007， 282(48)：34809-34816.
10. Yio XY， Mayer L. Characterization of a 180-kDa intestinalepithelial cell membrane glycoprotein， gp180. A candidate molecule mediating t cell-epithelial cell interactions[J]. J Biol Chem， 1997， 272(19)：12786-12792.
11. 孙仁山，陈晓红，冉新泽，等. 高亲和力IgE受体介导的信号传导及其相关的抑制性受体[J]. 中国药理学通报， 2005， 21(4)：385-389.
12. Wu J， Edberg JC， Redecha PB， et al. A novel polymorphism of Fc gamma RⅢa (CD16) alters receptor function and predisposes to autoimmune disease[J]. J Clin Invest， 1997， 100(5)：1059-1070.
13. Zhang W， Gordon M， Schultheis AM， et al. FCGR2A and FCGR3A polymorphisms associated with clinical outcome of epidermal growth factor receptor expressing metastatic colorectal cancer patients treated with single-agent cetuximab[J]. J Clin Oncol， 2007， 25(24)：3712-3718.
14. Senga T， Iwamoto S， Yoshida T， et al. LSSIG is a novel murineleukocyte-specific GPCR that is induced by the activation of STAT3[J]. Blood， 2003， 101(3)：1185-1187.
15. Nilsson NE， Kotarsky K， Owman C， et al. Identification of a free fatty acid receptor， FFA2R， expressed on leukocytes and activated by short-chain fatty acids[J]. Biochem Biophys Res Commun， 2003， 303(4)：1047-1052.
16. Karaki S， Tazoe H， Hayashi H， et al. Expression of the short-chain fatty acid receptor， GPR43， in the human colon[J]. J Mol Histol， 2008， 39(2)：135-142.
17. Tang Y， Chen Y， Jiang H， et al. G-protein-coupled receptor for short-chain fatty acids suppresses colon Cancer[J]. Int J Cancer，.
18. Pashine A， Busch R， Belmares MP， et al. Interaction of HLA-DRwith an acidic face of HLA-DM disrupts sequence-dependentinteractions with peptides[J]. Immunity， 2003， 19(2)：183-192.
19. Brocke P， Garbi N， Momburg F， et al. HLA-DM， HLA-DO and tapasin：functional similarities and differences[J]. Curr Opin Immunol， 2002， 14(1)：22-29.
20. Callahan MJ， Nagymanyoki Z， Bonome T， et al. Increased HLA-DMB expression in the tumor epithelium is associated with increased CTL infiltration and improved prognosis in advanced-stage serous ovarian cancer[J]. Clin Cancer Res， 2008， 14(23)：7667-7673.
21. Seung S， Urban JL， Schreiber H. A tumor escape variant that has lost one major histocompatibility complex class I restriction element induces specific CD8+T cells to an antigen that no longer serves as a target[J]. J Exp Med， 1993， 178(3)：933-940.
22. Arboleda MJ， Lyons JF， Kabbinavar FF， et al. Overexpression of AKT2/protein kinase Bbeta leads to up-regulation of beta1integrins， increased invasion， and metastasis of human breast and ovarian cancer cells[J]. Cancer Res， 2003， 63(1)：196-206.
23. Zhu JW， Brdicka T， Katsumoto TR， et al. Structurally distinct phosphatases CD45 and CD148 both regulate B cell and macrophage immunoreceptor signaling[J]. Immunity， 2008， 28(2)：183-196.
24. Penninger JM， Irie-Sasaki J， Sasaki T， et al. CD45：new jobs for an old acquaintance[J]. Nat Immunol， 2001， 2(5)：389-396.
25. ， 128(4)：847-856.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Change of Immunologic Gene Expression in Cases of Colorectal Cancer with Liver Metastasis by DNA Microarray

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