• 1.Department of General Surgery, The General Military Hospital of Shenyang Commanding Area, Shenyang 110016, Liaoning Province,China;;
  • 2.Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi’an 710032,Shaanxi Province, China;;
  • 3.Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University,Xi’an 710032, Shaanxi Province, China;
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Objective  To explore the expression and function of NDRG2 gene in human primary hepatocellular carcinoma and normal hepatic tissues.
Methods  The immunohistochemical ABC method, Western blot, and Real-time PCR were used to investigate the expression and content of NDRG2 in human hepatocellular carcinoma and hepatic normal biopsies.
Results  The NDRG2 protein located in cytoplasm. The positive rate was 16.67%(5/30) and 100%(30/30) in hepatocellular carcinoma and normal hepatic tissues, respectively. The relative content of NDRG2 protein in hepatocellular carcinoma and normal hepatic tissues were 0.029 0±0.005 9 and 0.109 2±0.002 8. There were significant differences between human hepatocellular carcinoma and hepatic normal biopsies both in staining positive rates and relative content(P<0.05). The Western blot also agreed with the result,the expression level of NDRG2 protein in hepatocellular carcinoma and normal hepatic tissues was 1.13±0.15 and 1.57±0.18, respectively, there was significant difference(P<0.05). Also, compared with normal hepatic tissues, the expression level of NDRG2 mRNA in carcinoma tissues was reduced significantly (0.89±0.15 vs. 1.48±0.17, P<0.05). However, there were no significant differences in NDRG2mRNA expression between Edmondson-Steiner grades.
Conclusions  There possibly have difference in NDRG2 expression between human primary hepatocellular carcinoma and normal hepatic tissue. NDRG2 gene may take part in the pathogenesis of human primary hepatocellular carcinoma. Futher study will be needed to study its mechanism and function.

Citation: WU Guoqiang,ZHANG Jian,LI Kaizong,DOU Kefeng,LIU Xinping.. The Expression and Significance of NDRG2 Gene in Primary Hepatocellular Carcinoma and Normal Hepatic Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(3): 266-270. doi: Copy

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