The Role of CIB1 in OX-LDL Inhibiting Migration of Mouse Macrophages_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHENG Jianghua , CHEN Kai

Department of Vascular Surgery, the affiliated hospital of Sichuan North Medical College, Nanchong 637000, Sichuan Province, China;

Corresponding author：

Keywords：

Calcium- and integrin-binding protein-1; RNA interference; Mouse macrophages; Oxidized low-density lipoprotein; Cell migration

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Objective 　To investigate the role of calcium- and integrin-binding protein-1（CIB1） in oxidized lowdensity lipoprotein（OX-LDL） inhibiting migration of mouse macrophages.
Methods 　To silence CIB1 express of mouse macrophages by RNA interference, then incubating mouse macrophages with OX-LDL, cell migration and cell spreading of mouse macrophages were analyzed.
Results 　At 24-72h after macrophages transfected CIB1 siRNA, the express of CIB1 protein was restrained obviously. To silence CIB1 express could increase migration and spreading of mouse macrophages significantly.
Conclusions 　CIB1 plays the important role in intracellular modulating mechanism of OX-LDL inhibiting mouse macrophages migration.

Citation： ZHENG Jianghua,CHEN Kai,.. The Role of CIB1 in OX-LDL Inhibiting Migration of Mouse Macrophages. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(6): 622-626. doi: Copy

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17.	Barlic J， Zhang Y， Murphy PM. Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNF-NFB-dependent manner [J]. J Biol Chem， 2007，282(26)：19167-19176.
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21.	Yamniuk AP， Nguyen LT， Hoang TT， et al. Metal ion binding properties and conformational states of calcium- and integrinbinding protein [J]. Biochemistry， 2004， 43(9)：2558-2568.
22.	石洁，惠玲，张煦，等．钙整合素结合蛋白CIB 的原核表达及多克隆抗体的制备与亚细胞定位 [J]．细胞与分子免疫学杂志， 2009， 25(1)：58-61．.
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1. 唐朝枢．心脑血管疾病发病和防治的基础研究 [J]. 生命科学， 2006， 18(3)：199-208．.
2. Peter L. Inflammation in atherosclerosis [J]. Nature， 2002，420(6917)：868-874.
3. Seimon T， Tabas I. Mechanisms and consequences of macrophage apoptosis in atherosclerosis [J]. J Lipid Res， 2009， 50 (Suppl)：S382-S387.
4. Tabas I. Macrophage death and defective inflammation resolution in atherosclerosis [J]. Nat Rev Immunol， 2010， 10(1)：36-46.
5. Thorp E， Tabas I. Mechanisms and consequences of efferocytosis in advanced atherosclerosis [J]. J Leukoc Biol， 2009， 86(5)：1089-1095.
6. Platt N， Gordon S. Is the class A macrophage scavenger receptor (SR-A) multifunctional?—— The mouse’s tale [J]. J Clin Invest，2001， 108(5)：649-654.
7. Silverstein RL， Febbraio M. CD36 and atherosclerosis [J].Curr Opin Lipidol， 2000， 11(5)：483-491.
8. Angeli V， Llodrá J， Rong JX， et al. Dyslipidemia associated with atherosclerotic disease systemically alters dendritic cell mobilization [J]. Immunity， 2004， 21(4)：561-574.
9. Randolph GJ. Emigration of monocyte-derived cells to lymph nodes during resolution of inflammation and its failure in atherosclerosis [J]. Curr Opin Lipidol， 2008， 19(5)：462-468.
10. Pentikäinen MO， Oksjoki R， Oörni K， et al. Lipoprotein lipase in the arterial wall：linking LDL to the arterial extracellular matrix and much more [J]. Arterioscler Thromb Vasc Biol， 2002，22(2)：211-217.
11. Watanabe N， Ikeda U. Matrix metalloproteinases and atherosclerosis [J]. Curr Atheroscler Rep， 2004， 6(2)：112-120.
12. Curtiss LK. Reversing atherosclerosis? [J]. N Engl J Med，2009， 360(11)：1144-1146.
13. Leisner TM， Liu M， Jaffer ZM， et al. Essential role of CIB1 in regulating PAK1 activation and cell migration [J]. J Cell Biol，2005， 170(3)：465-476.
14. 郭志刚，吴平生，李建华，等．巨噬细胞ABCA1 对Ox-LDL 诱导的炎症因子的调节及其意义 [J]．中山大学学报( 医学科学版)， 2007， 28(1)：6-10．.
15. Williams KJ， Feig JE， Fisher EA. Rapid regression of atherosclerosis：insights from the clinical and experimental literature[J]. Nat Clin Pract Cardiovasc Med， 2008， 5(2)：91-102.
16. Barlic J， Zhang Y， Foley JF， et al. Oxidized lipid-driven chemokine receptor Switch， CCR2 to CX3CR1， mediates adhesion of human macrophages to coronary artery smooth muscle cells through a peroxisome proliferator-activated receptor gammadependent pathway [J]. Circulation， 2006， 114(8)：807-819.
17. Barlic J， Zhang Y， Murphy PM. Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNF-NFB-dependent manner [J]. J Biol Chem， 2007，282(26)：19167-19176.
18. Park YM， Febbraio M， Silverstein RL. CD36 modulates migration of mouse and human macrophages in response to oxidized LDL and may contribute to macrophage trapping in the arterial intima [J]. J Clin Invest， 2009， 119(1)：136-145.
19. Wei C， Wang X， Chen M， et al. Calcium flickers steer cell migration [J]. Nature， 2009， 457(7231)：901-905.
20. Tong XP， Li XY， Zhou B， et al. Ca2＋ signaling evoked by activation of Na＋ channels and Na＋/Ca2＋ exchangers is required for GABA-induced NG2 cell migration [J]. J Cell Biol， 2009，186(1)：113-128.
21. Yamniuk AP， Nguyen LT， Hoang TT， et al. Metal ion binding properties and conformational states of calcium- and integrinbinding protein [J]. Biochemistry， 2004， 43(9)：2558-2568.
22. 石洁，惠玲，张煦，等．钙整合素结合蛋白CIB 的原核表达及多克隆抗体的制备与亚细胞定位 [J]．细胞与分子免疫学杂志， 2009， 25(1)：58-61．.
23. Zayed MA， Yuan W， Chalothorn D， et al. Tumor growth and angiogenesis is impaired in CIB1 knockout mice [J]. J Angiogenes Res， 2010， 2：17.
24. Zayed MA， Yuan W， Leisner TM， et al. CIB1 regulates endothelial cells and ischemia-induced pathological and adaptive angiogenesis [J]. Circ Res， 2007， 101(11)：1185-1193.
25. Kostyak JC， Naik MU， Naik UP. Calcium- and integrin-binding protein 1 regulates megakaryocyte ploidy， adhesion， and migration [J]. Blood， 2012， 119(3)：838-846.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Role of CIB1 in OX-LDL Inhibiting Migration of Mouse Macrophages

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